›› 2007, Vol. 34 ›› Issue (1): 119-123.

• 流行病防治 • 上一篇    下一篇

多重耐药鸡致病性沙门氏菌I类整合子的检测研究

李成忠1, 王红宁2, 黄勇3, 马孟根2, 吴琦4,羊云飞3，谢涛3，柳萍3
   

  1. 1.四川农业大学资环学院，雅安 625014；2. 四川大学“985工程”西南资源环境与灾害防治科技创新平台，成都 610061；3.四川农业大学动物科技学院，雅安 625014；4.四川农业大学生命理学院，雅安 625014
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-20 发布日期:2007-01-20

Study on Detection I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken

LI Chengzhong1, WANG Hongning2 ,HUANG Yong3, MA Menggen2, WU Qi4,YANG Yunfei3, XIE Tao3, LIU Ping3
   

  1. 1.College of Resources and Environmental Science ,Sichuan Agricultural University,Yaan 625014, China; 2.College of Life Science ,Sichuan University,Chengdu 610064, China； 3.College of Animal Science and Technology,Sichuan Agricultural University,Yaan 625014, China;4.College of Life Science and Science,Sichuan Agricultural University,Yaan 625014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-20 Published:2007-01-20

摘要： 采用药敏纸片法（NCCLS 2004），对19株鸡致病性沙门氏菌进行了药敏试验，结果19株菌对丁胺卡那霉素、卡那霉素、庆大霉素、新霉素、链霉素、氨苄青霉素、强力霉素、氟哌酸、头孢三嗪、氯霉素、磺胺异恶唑、四环素、先锋V 13种药物的耐药率分别是：5.26%、63.16%、47.37%、36.84%、84.21%、84.2%、100%、57.9%、5.26%、52.6%、89.47%、100%、26.3%。19株菌多为多重耐药：其中耐5种、6种、7种、8种、9种、10种药物的分别为10.53%、 5.26%、15.79%、21.05%、15.79%、15.79%，多重耐药率84.21%（16/19）；根据I类整合子的结构设计了３对引物，对I类整合子的intI1、可变区、3’保守端进行扩增，检测鸡致病性沙门氏菌I类整合子,分析I类整合子与多重耐药的关系，扩增出了750～2000 bp的基因盒片段，检出率为73.68%（14/19），多重耐药菌的I类整合子检出率为87.5%（14/16），I类整合子PCR阳性菌株多重耐药率为100%（14/14）。研究结果表明，I类整合子与多重耐药之间有明显的相关性。

关键词: 沙门氏菌; I类整合子; 多重耐药; 检测

Abstract: Antimicrobial resistance test of 19 samonella isolates was done by disc dilution method(NCCLS 2003).According to the construction of I integron , The drugresistance rate of amikacin,kanamycin, gentamicin,neomycin ,streptomycin, ampicillin, doxycycline，norfloxacin, ceftriaxone, chloramphenicol, sulfafurazole, tetracycline, cefazolin，was 5.26%,63.16%,47.37%,36.84%,84.21%,84.2%,100%,57.9%,5.26%,52.6%,89.47%,100%, 26.3% respectively. Most of the isolates were multidrug resistance and the rate was 84.21%(16/19).Among the total of the multidrugresistance isolates,the rate of resistance to five ,six, seven, eight ,nine and ten drugs was 10.53%，5.26%，15.79%，21.05%，15.79%， 15.79%. PCR was employed to amplify I integron and analysis the relationship between I integron and multidrug resistance. 3 pairs of primer was design to amplify I integron's intI1 gene, variable segment and 3'conserved segment. As a result, we amplified several fragments with different length from 750 to 2000 bp. Among these 19 isolates, the detection rate of I integron was 73.68%（14/19）; and among these 16 isolates which had been proven multidrugresistant,the detection rate of I integron was 87.5%.The I integron positive isolates' rate of multidrugresistance was 100%(14/14) . The results shows I integron obviously has the relationship with multidrug resistance . 

Key words: samonella; I integron; multidrug resistance; detection

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