绿茶浸提液对青脚麻鸡消化酶活性、营养物质表观代谢率和血清生化指标的影响

doi:10.16431/j.cnki.1671-7236.2025.06.009

摘要/Abstract

摘要： 【目的】试验旨在探究饮用不同水平绿茶浸提液对青脚麻鸡消化酶活性、营养物质表观消化率和血清生化指标的影响，为废弃茶叶作为家禽生产中新型饲料添加剂的潜在应用开辟新的可行途径。【方法】试验随机选取1日龄青脚麻鸡400 只，分为4组，组内设置5个重复，每个重复20只鸡。各组鸡均饲喂基础饲粮，对照组鸡自由饮用清水，试验Ⅰ、Ⅱ和Ⅲ组鸡分别饮用0.3%、0.6%和1.2%的绿茶浸提液，试验期21 d。试验结束后，从每个重复中随机选取2只鸡采血、屠宰，收集胰腺和十二指肠食糜，检测血清生化指标和肠道消化酶活性；在试验的第19、20和21天，以重复为单位采集鸡的排泄物，测定养分表观代谢率。【结果】各试验组鸡胰腺脂肪酶活性均显著低于对照组(P＜0.05)，试验Ⅱ和Ⅲ组鸡十二指肠食糜胰蛋白酶活性显著高于对照组(P＜0.05)，试验Ⅲ组鸡十二指肠食糜脂肪酶活性显著低于对照组(P＜0.05)。试验Ⅱ和Ⅲ组鸡的粗蛋白质表观代谢率显著高于对照组(P＜0.05)。与对照组相比，各试验组鸡血清中甘油三酯(TG)含量显著降低(P＜0.05)；试验Ⅱ和Ⅲ组鸡血清尿素氮(BUN)、低密度脂蛋白(LDLC)含量和谷丙转氨酶(ALT)活性显著降低(P＜0.05),且BUN含量显著低于试验Ⅰ组(P＜0.05)，试验Ⅲ组鸡LDLC含量和ALT活性显著低于试验Ⅰ组(P＜0.05)。【结论】饮用绿茶浸提液可以提高青脚麻鸡粗蛋白质的表观代谢率和肠道蛋白酶的活性，并对血清蛋白质和脂类代谢指标产生有利影响，推荐饮用0.6%绿茶浸提液为宜。

关键词: 绿茶浸提液; 青脚麻鸡; 表观消化率; 消化酶活性; 血清生化指标

Abstract: 【Objective】 The aim of this experiment was to investigate the effects of different concentrations of tea extract in drinking water on digestive enzyme activities,nutrient apparent metabolism rate and serum biochemical indices of Cyan-shank Partridge chickens,so as to open up a new feasible way for the potential application of waste tea as a novel feed additive in poultry production.【Method】 A total of 400 one-day-old Cyan-shank Partridge chickens were randomly distributed into four groups,with 5 replicates in each group and 20 chickens in each replicate.Chickens in each group were fed the same basal diet.The chickens in control group drank water freely，and the chickens in experimental groups Ⅰ,Ⅱ and Ⅲ drank water with 0.3%,0.6% and 1.2% green tea aqueous extract,respectively.The experiment lasted for 21 days.At the end of the experiment,two chickens were randomly selected from each replicate for blood sampling,slaughtering,and collecting pancreas and duodenum to determine serum biochemical indices and intestinal digestive enzyme activities.At 19,20 and 21 days,the fresh feces of chickens were collected in the unit of repetition to determine the apparent metabolism rate of feed nutrients.【Result】 Compared with control group,the activities of pancreatic lipase of chickens in each experimental group was significantly lower (P＜0.05),the activity of duodenal trypsin in experimental groups Ⅱ and Ⅲ were significantly higher (P＜0.05),and the activity of duodenal lipase in experimental group Ⅲ was significantly lower (P＜0.05).The apparent metabolism rate of crude protein in experimental groups Ⅱ and Ⅲ were significantly higher (P＜0.05).Compared with control group,the serum triglyceride (TG) content of chickens was significantly lower in all experimental groups (P＜0.05),the serum urea nitrogen(BUN),low density lipoprotein cholesterol (LDLC) content and the activity of alanine aminotransferase (ALT) were significantly lower in experimental groups Ⅱ and Ⅲ(P＜0.05).The serum BUN content in experimental groups Ⅱ and Ⅲ was lower than that of the experimental group Ⅰ(P＜0.05),the serum LDLC content and the activity of ALT in experimental group Ⅲ were significantly lower than that of experimental groupⅠ(P＜0.05).【Conclusion】 The consumption of tea extract could increase the apparent metabolism rate of crude protein and the activity of intestinal trypsin of Cyan-shank Partridge chickens.Additionally,it had a favourable effect on serum protein and lipid metabolism indexes,and it was recommended to drink 0.6% green tea extract for Cyan-shank partridge chickens.

Key words: green tea aqueous extract; Cyan-shank Partridge chickens; apparent digestibility; digestive enzyme activity; serum biochemical indices

中图分类号:

S816.7

汤莉, 邓凯伟, 何书海, 李军, 秦清明, 梁成成, 吴海港, 韩旭, 鲁佳宁. 绿茶浸提液对青脚麻鸡消化酶活性、营养物质表观代谢率和血清生化指标的影响[J]. 中国畜牧兽医, 2025, 52(6): 2552-2560.

TANG Li, DENG Kaiwei, HE Shuhai, LI Jun, QIN Qingming, LIANG Chengcheng, WU Haigang, HAN Xu, LU Jianing. Effects of Green Tea Aqueous Extract on Nutrient Apparent Metabolism Rate,Digestive Enzyme Activities and Serum Biochemical Indices of Cyan-shank Partridge chickens[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2552-2560.

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