外源性RFRP-3和MLT对雄鼠初情期生长及繁殖性能的影响

doi:10.16431/j.cnki.1671-7236.2022.07.020

摘要/Abstract

摘要： 【目的】探究外源性RF-酰胺相关肽3（RF-amide related peptide 3，RFRP-3）和褪黑素（melatonin，MLT）对雄性昆明小鼠初情期生长、繁殖性能的影响及两者之间的关系。【方法】以初情期雄性昆明小鼠为研究对象，单独给予雄鼠生理盐水、外源性RFRP-3或MLT以及混合给予RFRP-3、MLT，检测其对雄鼠平均增重、平均采食量、料重比、睾丸发育情况，血清生长激素（growth hormone，GH）、黄体生成素（luteinizing hormone，LH）水平，以及生长、生殖相关基因表达的影响。【结果】外源性RFRP-3能抑制雄鼠GH基因表达，并抑制体重增长，提高料重比；而外源性MLT促进雄鼠GH基因表达，使体重增长较快，料重比降低；RFRP-3和MLT共同处理下，GH基因表达增加，体重增长速度与单独给予MLT组接近。RFRP-3可抑制褪黑素受体1A（melatonin receptor 1A，Mtnr1A）、LH基因表达及睾酮分泌，小鼠睾丸各级生精细胞和间质细胞数量减少；MLT可促进Mtnr1A、LH基因表达及睾酮分泌，睾丸间质细胞密度极显著增加（P＜0.01）；MLT和RFRP-3共同处理下，小鼠睾丸各级生精细胞和间质细胞数量减少，睾酮分泌显著减少（P＜0.05）。【结论】外源性RFRP-3能一定程度抑制雄性小鼠初情期的生长及繁殖性能，外源性MLT可促进初情期雄性小鼠的生长及繁殖性能。

关键词: RFRP-3基因; MLT基因; 雄鼠; 生长性能; 繁殖性能

Abstract: 【Objective】 This experiment was conducted to investigate the effects of exogenous RF-amides related peptide 3 (RFRP-3) and melatonin (MLT) on the growth and reproductive performance of male Kunming mice at the beginning of estrus,and the relationship between them.【Method】 Male Kunming mice at the beginning of estrus were given normal saline,exogenous RFRP-3,MLT,and a mixture of RFRP-3 and MLT,respectively.The effects on average weight gain,average feed intake,feed gain ratio (F/G),testicular development,the secretion levels of serum growth hormone (GH) and luteinizing hormone (LH),and the relative expression of growth and reproduction-related genes in mice were examined.【Result】 Exogenous RFRP-3 inhibited the expression of GH gene,suppressed body weight gain,and improved F/G;While exogenous MLT promoted the expression of GH gene,resulting in faster body weight growth and lower F/G.Under the combined treatment of RFRP-3 and MLT,the expression of GH gene increased,and the body weight gain was similar to that of MLT group.RFRP-3 inhibited the expression of melatonin receptor 1A (Mtnr1A) and LH genes and the secretion of testosterone,and the number of spermatogonia at all levels and leydig cells in the testes of mice decreased;MLT promoted the expression of Mtnr1A and LH genes and the secretion of testosterone,and the leydig cell density extremely significantly increased (P＜0.01).The MLT and RFRP-3 cotreatment decreased the number of spermatogonia and leydig cells,and significantly decreased the secretion of testosterone (P＜0.05).【Conclusion】 Exogenous RFRP-3 inhibited the growth and reproductive performance of male mice at the puberty,while exogenous MLT promoted the growth and reproductive performance of male mice.

Key words: RFRP-3 gene; MLT gene; male mice; growth performance; reproductive performance

中图分类号:

S814.1

潘建秋, 刘付穗, 吴曼莉, 江丹莉, 欧阳宏佳, 沈栩, 许丹宁, 贺建华, 黄运茂. 外源性RFRP-3和MLT对雄鼠初情期生长及繁殖性能的影响[J]. 中国畜牧兽医, 2022, 49(7): 2622-2630.

PAN Jianqiu, LIUFU Sui, WU Manli, JIANG Danli, OUYANG Hongjia, SHEN Xu, XU Danning, HE Jianhua, HUANG Yunmao. Effects of Exogenous MLT and RFRP-3 on Growth and Reproductive Performance of Male Mice at the Puberty[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2622-2630.

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