柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2020.09.014

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (9): 2816-2823.doi: 10.16431/j.cnki.1671-7236.2020.09.014

柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响

李贞明^1,2, 余苗¹, 崔艺燕¹, 容庭¹, 刘志昌¹, 杜宗亮^1,2, 马现永¹

1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东省畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
2. 清远市龙发种猪有限公司, 英德 511500

收稿日期:2020-03-13 出版日期:2020-09-20 发布日期:2020-09-16
通讯作者: 马现永 E-mail:maxianyong@gdaas.cn
作者简介:李贞明(1990-),男,山东临沂人,硕士,研究方向:生态健康养殖,E-mail:lzm900120@163.com
基金资助:
"十三五"国家重点研发计划（2016YFD0501210）；广东省畜禽育种与营养研究重点实验室开放运行课题（2017B030314044）；广东省现代农业产业技术体系创新团队（2017LM1080）；清远市创新创业科研团队项目（2018002）

Effects of Citrus Extract on Plasma Biochemical Indexes,Hormone Levels and Antioxidant Function of Broilers after Transport Stress

LI Zhenming^1,2, YU Miao¹, CUI Yiyan¹, RONG Ting¹, LIU Zhichang¹, DU Zongliang^1,2, MA Xianyong¹

1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Public Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Qingyuan Longfa Pig Breeding Co., Ltd., Yingde 511500, China

Received:2020-03-13 Online:2020-09-20 Published:2020-09-16

摘要/Abstract

摘要： 试验旨在研究柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响。选取360只28日龄快大型黄羽肉鸡，随机分2组（每组6个重复，每个重复30只鸡），对照组饲喂不含抗生素的基础日粮，试验组饲喂添加10 mg/kg柑橘提取物的基础日粮。63日龄，从每个重复选6只鸡进行运输应激试验，分别运输0、2、4 h，运输结束后翅静脉采血取样。结果表明：①对葡萄糖（GLU）、胆固醇（CHOL）和尿素氮（BUN）而言，日粮处理和运输时间存在显著交互作用（P<0.05）。与对照组相比，试验组肉鸡血浆中BUN和CHOL含量显著低于对照组，运输2和4 h，试验组肉鸡血浆中GLU含量显著高于对照组（P<0.05）；运输应激显著影响对照组肉鸡血浆中BUN、CHOL和GLU含量（P<0.05），随着运输时间的延长，BUN和CHOL含量逐渐提高，而GLU含量逐渐降低。②对于皮质酮（CORT）、三碘甲状腺原氨酸（T₃））和甲状腺素（T₄）而言，日粮处理和运输时间存在显著交互作用（P<0.05）。与对照组相比，运输2和4 h，试验组肉鸡血浆中CORT含量显著降低，而T₃和T₄含量显著提高（P<0.05）；运输应激显著影响对照组肉鸡血浆中CORT、T₃和T₄含量，运输后，肉鸡血浆中CORT含量显著提高，而T₃和T₄含量显著降低（P<0.05）。③对于谷胱甘肽过氧化物酶（GSH-Px）而言，日粮处理和运输时间存在显著交互作用（P<0.05）。与对照组相比，试验组肉鸡血浆中GSH-Px活性显著高于对照组（P<0.05）；运输应激显著影响对照组肉鸡血浆中GSH-Px活性，随着运输时间的延长，其活性显著降低（P<0.05）。综上，运输应激影响黄羽肉鸡机体的代谢水平、激素分泌和抗氧化功能，日粮中添加柑橘提取物可改善肉鸡的代谢水平，调控激素分泌和提高抗氧化能力，缓解运输应激对黄羽肉鸡的影响。

关键词: 柑橘提取物; 肉鸡; 运输应激; 激素; 抗氧化

Abstract: In order to study the effects of citrus extract on the plasma biochemical indexes,hormone level and antioxidant function of broilers after transport stress,a total of 360 twenty-eight-day-old fast-growing Yellow feather broilers were randomly divided into two groups (6 replicates per group,30 per replicate).The control group was fed basic diet without antibiotics,the experimental group was fed basic diet supplemented with 10 mg/kg citrus extract.At 63 days,six chickens were selected from each replicate for transport stress test for 0,2,and 4 h,respectively,and plasma sampling were conducted after transportation.The results showed that:① For glucose,cholesterol and urea nitrogen,there was a significant interaction between food treatment and transportation time (P<0.05).Compared with the control group,the contents of urea nitrogen and cholesterol in the plasma of broilers in the experimental group were significantly lower than that in the control group,and the content of glucose in the plasma of broilers in the experimental group was significantly higher than that in the control group (P<0.05).Transport stress significantly affected the content of urea nitrogen,cholesterol and glucose in the plasma of broilers in the control group,after transportation,the content of urea nitrogen and cholesterol increased significantly,while the content of glucose decreased significantly (P<0.05).② For corticosterone,triiodothyronine and thyroxine,there was significant interaction between food treatment and transport time (P<0.05).Compared with the control group,the content of corticosterone in the plasma of broilers in the experimental group was significantly lower than that in the control group,while the content of triiodothyronine and thyroxine in the experimental group was significantly higher than that in the control group (P<0.05).Transport stress significantly affected the content of corticosterone,triiodothyronine and thyroxine in the plasma of the control group,after transportation,the content of corticosterone in the plasma of the broiler significantly increased,while the content of triiodothyronine and thyroxine significantly decreased (P<0.05).③ For glutathione peroxidase,there was a significant interaction between food treatment and transportation time (P<0.05).Compared with the control group,the glutathione peroxidase activity in the plasma of broilers in the experimental group was significantly higher than that in the control group (P<0.05);Transport stress significantly affected the activity of glutathione peroxidase in the plasma of broilers in the control group.With the prolongation of transport time,the activity of glutathione peroxidase in the plasma of broilers decreased significantly (P<0.05).Therefore,transportation stress affected the metabolism level,hormone secretion and antioxidant function of Yellow feather broiler.Adding citrus extract to the diet could improve the metabolism level,regulate hormone secretion and improve antioxidant capacity,and alleviate the impact of transportation stress on Yellow feather broiler.

Key words: citrus extract; broiler; transport stress; hormone; antioxidant

中图分类号:

S816.7

李贞明, 余苗, 崔艺燕, 容庭, 刘志昌, 杜宗亮, 马现永. 柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响[J]. 中国畜牧兽医, 2020, 47(9): 2816-2823.

LI Zhenming, YU Miao, CUI Yiyan, RONG Ting, LIU Zhichang, DU Zongliang, MA Xianyong. Effects of Citrus Extract on Plasma Biochemical Indexes,Hormone Levels and Antioxidant Function of Broilers after Transport Stress[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2816-2823.

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柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响

Effects of Citrus Extract on Plasma Biochemical Indexes,Hormone Levels and Antioxidant Function of Broilers after Transport Stress

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