植物乳杆菌发酵培养物对单增李斯特菌感染肉鸡的治疗效果及机制

doi:10.16431/j.cnki.1671-7236.2020.08.037

摘要/Abstract

摘要： 本试验研究了植物乳杆菌发酵培养物对单增李斯特菌感染肉仔鸡的生产性能、氧化反应及促分裂原活化蛋白激酶（MAPK）信号通路的影响，旨在探讨植物乳杆菌发酵培养物对单增李斯特菌感染肉鸡的治疗效果及机制。选择1日龄AA公雏480只，随机分为4组，每组6个重复，每个重复20只鸡。对照组和感染组饲喂基础日粮，抗生素组、乳杆菌培养物组在基础日粮中分别添加40 mg/kg盐酸恩诺沙星和1.6 g/kg灭活植物乳杆菌培养物。5日龄时，感染组、抗生素组和乳杆菌培养物组每只鸡灌服1 mL（10⁵ CFU）单增李斯特菌感染液，对照组灌服无菌株培养液，全程试验期28 d。结果显示，与感染组相比，植物乳杆菌培养物可以显著提高7和28 d肉仔鸡平均日增重、平均日采食量和始末体重（P<0.05），显著降低肉仔鸡料重比（P<0.05）；显著降低14和28 d肉仔鸡血清和十二指肠黏膜中的二胺氧化酶、丙二醛、蛋白羰基（除14 d十二指肠黏膜外）的含量（P<0.05）；显著降低7和28 d肉仔鸡MAPK3、MAPK10和DUSP6（7 d除外）基因mRNA表达水平（P<0.05）。综上，日粮中添加植物乳杆菌发酵培养物可以提高肉鸡抗氧化性，抑制单增李斯特菌的感染，提高抗菌能力，通过MAPK信号提高抗炎能力。植物乳杆菌发酵培养物对单增李斯特菌感染肉鸡有较好的治疗效果，可以作为替代抗生素的添加剂。

关键词: 植物乳杆菌发酵培养物; 单增李斯特菌; 生产性能; 氧化反应; MAPK信号通路

Abstract: The effects of Lactobacillus plantarum (L.plantarum) culture on the growth performance,oxidative reaction and MAPK signaling pathway of broilers infected with Listeria monocytogenes (L.monocytogenes) were investigated in this study for exploring the efficacy of L.plantarum culture against listeriosis and possible mechanism.A total of 480 one day old AA male broiler chicks were randomly allocated into 4 groups including 6 replicates per group of 20 chicks each replicate.Chicks in control and infection groups were offered a basal diet,whereas other two groups were supplied antibiotic enrofloxacin hydrochloride at 40 mg/kg or inactivated L.plantarum culture at 1.6 g/kg.Feeding trial lasted for 28 days.At 5 days old,chicks in infection,antibiotic and L.plantarum culture groups were orally administrated 1 mL L.monocytogenes (10⁵ CFU),whereas chicks in control group were offered the same liquid without the pathogen.The results showed that L.plantarum culure significantly improved ADWG,ADFI and final body weight of 7 and 28 days old broilers (P<0.05),significantly decreased feed/gain ratio (P<0.05),significantly decreased serum and duodenal mucosa concentrations of diamine oxidase,malondialdehyde and protein carbonyls (except for 14 d in duodenal mucosa) of 7 and 28 days old broilers (P<0.05),and significantly decreased mRNA levels of MAPK3,MAPK10 and DUSP6 (except for 7 d) genes of 7 and 28 days old broilers (P<0.05).The results suggested that dietary L.plantarum culture could inhibit L.monocytogenes infection,and improve antibacterial,antioxidative ability and anti-inflammatory ability through MAPK signaling.L.plantarum culture had a good therapeutic effect on L.monocytogenes infected broilers,and could be used as an alternative for antibiotics.

Key words: Lactobacillus plantarum culture; Listeria monocytogenes; growth performance; oxidative action; MAPK signaling

中图分类号:

S852.61

郑江平, 刘宁, 白雪瑞, 尚月丽. 植物乳杆菌发酵培养物对单增李斯特菌感染肉鸡的治疗效果及机制[J]. 中国畜牧兽医, 2020, 47(8): 2666-2676.

ZHENG Jiangping, LIU Ning, BAI Xuerui, SHANG Yueli. Effects and Mechanism of Lactobacillus plantarum Culture Against Listeria monocytogenes Infection in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2666-2676.

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