抗菌肽添加量对杂交鲟生化指标的影响

doi:10.16431/j.cnki.1671-7236.2020.05.019

摘要/Abstract

摘要： 为研究抗菌肽添加量对杂交鲟血清和组织免疫相关指标和抗损伤能力的影响。挑选体格健壮、大小一致的杂交鲟（俄罗斯鲟Acipenser gueldenstaedtii♀×史氏鲟Acipenser schrencki Brandt♂）200尾，随机分成4组。对照组投喂基础饲料，Ⅰ、Ⅱ、Ⅲ组分别在基础饲料中添加400、800和1 200 mg/kg的抗菌肽，在（16.0±1.0）℃水温条件下养殖30 d。结果表明，摄食添加抗菌肽的杂交鲟，其血清和组织中白蛋白（ALB）和总蛋白（TP）含量随抗菌肽质量浓度的增加先增后减，其中Ⅱ组杂交鲟血清和组织中ALB和TP含量显著高于对照组（P<0.05）。血清中谷草转氨酶（GOT）和乳酸脱氢酶（LDH）活性随抗菌肽质量浓度的增加先降低后增加；Ⅰ组杂交鲟血清和组织中GOT活性显著低于其他组（P<0.05）；而Ⅲ组GOT和LDH活性显著高于其他组（P<0.05）。杂交鲟组织中总胆固醇（TCHO）含量随抗菌肽质量浓度的增加逐渐减少，Ⅲ组TCHO含量在组织中显著低于其他组（P<0.05）。溶菌酶（LZM）活性随抗菌肽质量浓度的增加而逐渐增加，Ⅱ、Ⅲ组杂交鲟心脏、脾脏和小肠中LZM活性显著高于其他组（P<0.05）。因此，在基础饲料中添加抗菌肽可有效提高杂交鲟的部分非特异性免疫能力和抗损伤能力，抗菌肽添加剂量在400~800 mg/kg时效果最佳。

关键词: 抗菌肽; 生化指标; 杂交鲟; 免疫能力

Abstract: In order to study the effect of the amount of antimicrobial peptide on the immunity-related indicators and anti-injury ability of the serum and tissue of hybrid sturgeon.200 hybrid sturgeons(Russian sturgeon Acipenser gueldenstaedti♀×Amur sturgeon Acipenser schrencki Brandt♂) with robust physique and uniform size were selected and randomly divided into 4 groups.The fishes in control group were fed with basal diet,the fishes in experiment groups were fed with composite additive of antimicrobial peptides with level of 400 mg/kg (group Ⅰ)、800 mg/kg (group Ⅱ) and 1 200 mg/kg (group Ⅲ),respectively.The experiment lasted for 30 d at water temperature of (16.0±1.0) ℃.It was found that the contents of albumin (ALB) and total protein(TP) in serum and tissues were increased at first and then decreased with the increase of dietary antimicrobial peptides concentration,and the contents of ALB and TP in groupⅡ significantly higher than the control group (P<0.05).The activities of lactic dehydrogenase (LDH) and glutamic oxalacetic transaminase (GOT) were shown to be decreased at first and then increased with the increase of antimicrobial peptides concentration in serum.In serum and tissues the activity of GOT in groupⅠwas significantly lower than that in other groups (P<0.05),and the activity of GOT and LDH in group Ⅲ were significantly higher than those in other groups (P<0.05).The total cholesterol (TCHO) contents were gradually decreased with the increase of the concentration of antimicrobial peptides in tissues,and the TCHO contents in group Ⅲ were significantly lower in tissues than those in other groups (P<0.05).The activity of lysozyme (LZM) was gradually increased with the increase of the concentration of antimicrobial peptides,and the activity of LZM was significantly higher in groupⅡ and groupⅢ in heart,spleen and small intestine than that in other groups (P<0.05).The addition of antimicrobial peptides leaded to effectively improve the nonspecific immunity and the damage resistant ability of hybrid sturgeon,and antibacterial peptides had the best effect when the dosage was 400-800 mg/kg.

Key words: antimicrobial peptide; biochemical index; hybrid sturgeon; immunity ability

中图分类号:

S966.9

罗志成, 刘哲, 权金强, 康玉军, 秦勇. 抗菌肽添加量对杂交鲟生化指标的影响[J]. 中国畜牧兽医, 2020, 47(5): 1452-1458.

LUO Zhicheng, LIU Zhe, QUAN Jinqiang, KANG Yujun, QIN Yong. Effects of Additive Amount of Antimicrobial Peptides on Biochemical Indices of Hybrid Sturgeon[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1452-1458.

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