盘羊及其杂交后代微卫星遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2020.04.016

摘要/Abstract

摘要： 为了更好地了解盘羊及其杂交后代（盘羊♂×欧拉羊♀）的遗传信息，为盘羊遗传资源评价及欧拉羊的复壮改良提供理论依据。本试验选取24对微卫星引物，应用PCR扩增和毛细管电泳的方法对24只盘羊和24只杂交羊个体进行遗传多样性分析。结果显示，在24只盘羊中，24个微卫星位点上共检测到167个等位基因，平均等位基因数目为6.9583，平均有效等位基因数为3.3665，平均观测杂合度为0.4497，平均期望杂合度为0.6779，平均多态信息含量为0.6265，其中，等位基因数目最多的是位点MAF70，有效等位基因数目、期望杂合度、多态信息含量最高的是位点OarFCB193，观测杂合度最高的是位点ILSTS11；在24只杂交羊中，24个微卫星位点上共检测到154个等位基因，平均等位基因数目为6.4166，平均有效等位基因数为3.4061，平均观测杂合度为0.4566，平均期望杂合度为0.6751，平均多态信息含量为0.6265，其中，等位基因数目、有效等位基因数目、期望杂合度、多态信息含量最高的是位点MAF70，观测杂合度最高的是位点OarJMP58。从遗传学角度得出，盘羊和杂交羊遗传指标结果相近，试验所得数据可用于盘羊遗传资源评价及欧拉羊的复壮改良。

关键词: 盘羊; 欧拉羊; 微卫星; 遗传标记

Abstract: In order to better understand the genetic information of Argali sheep and its hybrid offspring (Argali sheep ♂×Oula sheep ♀),and provide a theoretical basis for the evaluation genetic resources of Argali sheep and the rejuvenation and improvement of Oula sheep,24 microsatellite loci were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 24 Argali sheep and 24 hybrid sheep.The results showed that,167 alleles were detected at 24 microsatellite loci in 24 Argali sheep,the average number of alleles was 6.9583,the average effective allele was 3.3665,the average observed heterozygosity was 0.4497,the average expected heterozygosity was 0.6779,and the average polymorphic information content was 0.6265,MAF70 locus had the highest numbers of alleles,OarFCB193 locus had the highest numbers of effective alleles,expected heterozygosity and polymorphism information content,ILSTS11 locus had the highest observe heterozygosity.In 24 hybrid sheep,154 alleles were found in 24 microsatellite loci,the average number of alleles was 6.4166,the average effective allele was 3.4061,the average observed heterozygosity was 0.4566,the average expected heterozygosity was 0.6751,and the average polymorphic information content was 0.6265.MAF70 locus had the highest numbers of alleles,numbers of effective alleles,expected heterozygosity and polymorphism information content,OarJMP58 locus had the highest observe heterozygosity.It was found that the genetic indicators of Argali sheep and hybrid offspring were similar in genetics,and the data could be used for evaluate the genetic resources of Argali sheep and the rejuvenation and improvement of Oula sheep.

Key words: Argali sheep; Oula sheep; microsatellite; genetic marker

中图分类号:

S813.3

迟浩斌, 郎侠, 王彩莲, 刘立山. 盘羊及其杂交后代微卫星遗传多样性分析[J]. 中国畜牧兽医, 2020, 47(4): 1109-1120.

CHI Haobin, LANG Xia, WANG Cailian, LIU Lishan. Analysis of Microsatellite Genetic Diversity in Argali and Its Hybrid Offspring[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1109-1120.

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