酵母多糖对空肠弯曲菌感染獭兔生产性能、载菌量和抗菌肽表达的影响

doi:10.16431/j.cnki.1671-7236.2020.04.015

摘要/Abstract

摘要： 试验旨在研究酵母多糖对空肠弯曲菌感染獭兔生产性能、载菌量、盲肠和皮肤抗菌肽的影响。选取断奶獭兔180只，随机分为5组，每组6个重复，每个重复6只。对照组饲喂基础日粮，其他4组空肠弯曲菌感染，并于基础日粮中分别添加0、200、400、600 mg/kg酵母多糖。饲养试验起始日诱导空肠弯曲菌感染，饲养试验持续35 d。结果显示，空肠弯曲菌感染降低了獭兔结束体重、日增重和日采食量，提高了料重比（P < 0.05），添加酵母多糖改善了这些指标（P < 0.05），且日增重和料重比达到了对照组水平。酵母多糖降低了獭兔盲肠食糜、皮肤、肝脏、脾脏的空肠弯曲菌载菌量（P < 0.05）。空肠弯曲菌感染诱导内源性抗菌肽的Cathelicidin、Galectin-3和LEAP2基因表达量显著上调（P < 0.05），对DEFA4基因无显著影响，而添加酵母多糖对基因表达量具有进一步上调作用（P < 0.05）。酵母多糖添加剂量与结束体重、日增重呈现线性和平方效应关系（P<0.05）；与皮肤、肝脏和脾脏载菌量呈现线性效应（P<0.05）；与盲肠和皮肤抗菌肽Cathelicidin和Galectin-3基因表达水平呈现线性效应（P<0.05）；与盲肠抗菌肽Galectin-3、LEAP2和DEFA4基因呈现平方效应（P<0.05）。表明日粮添加酵母多糖可以降低空肠弯曲菌感染獭兔组织载菌量，提高内源抗菌肽活性，不影响獭兔生产性能。

关键词: 酵母多糖; 空肠弯曲菌; 獭兔; 生长性能; 抗菌肽

Abstract: This study was aimed to investigate the effect of yeast polysaccharide (YPS) supplementation on growth performance,Campylobacter jejuni(C.jejuni) carriage and antimicrobial peptides in the epithelial tissue of cecum and skin in Rex rabbits.Five treatments included control and C.jejuni challenge with the addition of YPS at 0,200,400 or 600 mg/kg of diet.The trial lasted for 35 days and C.jejuni challenge was occurred at first day of feeding trial.The results showed that C.Jejuni challenge worsened final body weight,feed intake,body weight gain and feed efficiency (P < 0.05),whereas YPS supplementation compensated body weight gain and feed efficiency (P < 0.05).C.jejuni populations in the cecal digesta,skin,liver and spleen were decreased in the treatments containing YPS (P < 0.05). C.jejuni induced mRNA upregulation of Cathelicidin,Galectin-3 and LEAP2 gene (P < 0.05),but had no effect on DEFA4 gene,and addition of YPS further upregulated the gene expression (P < 0.05). Linear and quadratic trends of the three doses of YPS were found on final body weight and body weight gain (P<0.05),linear trends on C.jejuni carriage in tissues of skin,spleen and liver (P<0.05),linear trends on Cathelicidin and Galectin-3 in tissues of cecum and skin (P<0.05),and quadratic trends on Galectin-3,LEAP2 and DEFA4 gene in the cecal epithelium (P<0.05).It was concluded that YPS could effectively control C.jejuni infection by decreasing C.Jejuni carriage and activing endogenous epithelial antimicrobial peptides,and had no negative effect on the growth performance of rabbits.

Key words: yeast polysaccharide; Campylobacter jejuni; Rex rabbit; growth performance; antimicrobial peptide

中图分类号:

S829.1

郑江平, 刘宁, 白雪瑞, 张飞可. 酵母多糖对空肠弯曲菌感染獭兔生产性能、载菌量和抗菌肽表达的影响[J]. 中国畜牧兽医, 2020, 47(4): 1099-1108.

ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Yeast Polysaccharide on Growth Performance,Bacterial Load and Endogenous Antimicrobial Peptides in Rabbits Infected with Campylobacter jejuni Expression[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1099-1108.

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