富维生素E转基因玉米对肉鸡生长性能、生化指标及抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2019.09.014

摘要/Abstract

摘要： 试验旨在研究富维生素E（VE）抗虫抗除草剂转基因玉米（富VE转基因玉米）对肉鸡生长性能、生化指标及抗氧化功能的影响，并与非转基因玉米添加外源DL-α-生育酚乙酸酯作用效果比较，对富VE转基因玉米营养有效性进行评价。选用360只1日龄罗斯308肉鸡公雏，随机分为3组，每组6个重复，每个重复20只，试验期42 d。普通玉米负对照组（负对照组，NC）日粮使用非转基因玉米配制不添加外源VE，1~21和22~42 d日粮VE水平分别为4.38和4.63 IU/kg；富VE转基因玉米组（富VE组，VER）使用富VE转基因玉米配制，1~21和22~42 d日粮VE水平分别为14.11和14.91 IU/kg；普通玉米正对照组（正对照组，PC）使用非转基因玉米配制并添加DL-α-生育酚乙酸酯添加剂，使其VE水平与富VE组一致。结果表明：①除1~21 d富VE组平均日采食量（ADFI）显著低于负对照组外（P<0.05），其他生长性能各指标在各组间均无显著性差异（P>0.05）。② 21 d时，富VE组血清谷丙转氨酶（ALT）与正对照组相比无显著差异（P>0.05），与负对照组相比显著降低（P<0.05）；肝脏甘油三酯（TG）含量低于负对照组但无显著性差异（P>0.05），且两组均显著高于正对照组（P<0.05）；42 d时，富VE组肝脏TG含量与正对照组相比无显著性差异（P>0.05），且两组均显著低于负对照组（P<0.05）。③与正对照组相比，富VE组21 d血清丙二醛（MDA）、过氧化氢（H₂O₂）、还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）以及42 d血清MDA、H₂O₂、GSH、SOD、CAT和T-AOC均无显著性差异（P>0.05）；与负对照组相比，富VE组21 d和42 d血清MDA和H₂O₂均显著降低（P<0.05），21 d血清GSH、GSSG、谷胱甘肽过氧化物酶（GSH-Px）、SOD、CAT和T-AOC及42 d血清GSSG、GSH-Px、CAT和T-AOC均显著升高（P<0.05）。以上结果表明，同等VE水平条件下，富VE转基因玉米与非转基因玉米添加外源DL-α-生育酚乙酸酯添加剂对试验肉鸡生长性能、生化指标和抗氧化功能具有相似作用效果。

关键词: 富维生素E转基因玉米; 肉鸡; 生长性能; 生化指标; 抗氧化功能

Abstract: This study was conducted to investigate the effects of VE-riched transgenic maize on growth performance,biochemical parameters and antioxidant capacity of broilers,and evaluate the nutritional effectiveness of VE-riched transgenic maize compared with the non-genetically modified maize (non-GM maize) added exogenous DL-α-tocopherol acetate additive.360 1-day-old Ross 308 male broilers were randomly divided into 3 groups with 6 replicates and 20 chickens in per replicate.The study period was 42 d.The diet for ordinary maize negative control group (negative control group,NC) was prepared with non-GM maize without exogenous VE and the VE level of diets during 1 to 21 d and 22 to 42 d were 4.38 and 4.63 IU/kg,respectively;Diet for VE-riched transgenic maize group (VE-riched group,VER) was prepared with VE-riched transgenic maize and the VE level of diets during 1 to 21 d and 22 to 42 d were 14.11 and 14.91 IU/kg,respectively;Diet for ordinary maize positive control group (positive control group,PC) was prepared with non-GM maize added DL-α-tocopherol acetate additive to make VE level consistent with VE-riched group.The results showed as follows:①Except for average daily feed intake (ADFI) of 1 to 21 d in VE-riched group which was significantly lower than that in negative control group (P<0.05),there was no significant difference in other growth performance parameters among the three groups during the experiment (P>0.05).②On 21 d,the serum ALT of VE-riched group was not significantly different from positive control group (P>0.05),but significantly lower than that of negative control group (P<0.05),and there was no significant difference in liver TG content between VE-riched group and negative control group (P>0.05),and the two groups were significantly higher than that of positive control group (P<0.05);On 42 d,there was no significant difference in the liver TG content between VE-riched group and positive control group (P>0.05),and the two groups were significantly lower than that of negative control group (P<0.05);③Compared with positive control group,serum MDA,H₂O₂,GSH,GSSG,SOD,CAT and T-AOC of 21 d and serum MDA,H₂O₂,GSH,SOD,CAT and T-AOC of 42 d in VE-riched group had no significant difference (P>0.05);Compared with negative control group,serum MDA and H₂O₂ of 21 d and 42 d significantly decreased in VE-riched group (P<0.05),meanwhile,GSH,GSSG,GSH-Px,SOD,CAT and T-AOC of 21 d and GSSG,GSH-Px,CAT and T-AOC of 42 d increased significantly in the VE-riched group (P<0.05).The above results demonstrated that VE-riched transgenic maize and non-GM maize added with exogenous DL-α-tocopherol acetate additive had similar effects on growth performance,biochemical parameters and antioxidant capacity of the test broilers under the same VE level.

Key words: VE-riched transgenic maize; broiler; growth performance; biochemical parameters; antioxidant capacity

中图分类号:

S816.41

詹腾飞, 韩云胜, 汤超华, 赵青余, 张军民. 富维生素E转基因玉米对肉鸡生长性能、生化指标及抗氧化功能的影响[J]. 中国畜牧兽医, 2019, 46(9): 2608-2617.

ZHAN Tengfei, HAN Yunsheng, TANG Chaohua, ZHAO Qingyu, ZHANG Junmin. Effects of Vitamin E-riched Transgenic Maize on Growth Performance, Biochemical Parameters and Antioxidant Capacity of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2608-2617.

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