丁酸梭菌和乳酸菌对青年鸽免疫指标、血清抗氧化指标及肠道功能的影响

doi:10.16431/j.cnki.1671-7236.2019.07.011

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (7): 1969-1975.doi: 10.16431/j.cnki.1671-7236.2019.07.011

丁酸梭菌和乳酸菌对青年鸽免疫指标、血清抗氧化指标及肠道功能的影响

袁文华^1,2, 李国勤^1,3, 韩安法⁴, 王淼⁵, 李浙烽⁵, 赵威⁵, 刁新平², 卢立志^1,3

1. 浙江省农业科学院畜牧兽医研究所, 杭州 310021;
2. 东北农业大学动物科学技术学院, 哈尔滨 150030;
3. 农业农村部农产品信息溯源重点实验室, 杭州 310021;
4. 苍南恭喜养殖专业合作社, 温州 325000;
5. 杭州康德权饲料有限公司, 杭州 311107

收稿日期:2019-01-02 出版日期:2019-07-20 发布日期:2019-07-22
通讯作者: 刁新平, 卢立志 E-mail:diaoxp63@163.com;lulizhibox@163.com
作者简介:袁文华(1993-),女,河南周口人,硕士生,研究方向:单胃动物营养与饲料科学,E-mail:18846089683@163.com
基金资助:
浙江省农业（畜禽）新品种选育重大科技专项（2016C02054-16）；浙江省农业科学院地方科技合作项目（WZ2017002）；苍南县科技项目"益生菌调控青年鸽生产和免疫性状的作用研究

Effects of Clostridium butyrate and Lactobacillus on Immune Indexs, Serum Antioxidation Indexes and Intestinal Function in Young Pigeons

YUAN Wenhua^1,2, LI Guoqin^1,3, HAN Anfa⁴, WANG Miao⁵, LI Zhefeng⁵, ZHAO Wei⁵, DIAO Xinping², LU Lizhi^1,3

1. Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
3. Key Laboratory of Agricultural Products Information Traceability, Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China;
4. Breeding Cooperative of Cangnan Congratulations, Wenzhou 325000, China;
5. Hangzhou Kangdequan Feed Co., Ltd., Hangzhou 311107, China

Received:2019-01-02 Online:2019-07-20 Published:2019-07-22

摘要/Abstract

摘要：

本试验旨在研究丁酸梭菌和乳酸菌对青年鸽免疫性能、血清抗氧化指标、小肠消化酶活性和肠道形态的影响。选取80日龄左右的雌性青年鸽384只，将其随机分成4组，每组6个重复，每个重复16只。其中A组饲喂基础日粮，B、C和D组分别在基础日粮中添加1×10⁸ CFU/kg丁酸梭菌、5×10⁹ CFU/kg乳酸菌和5×10⁹ CFU/kg乳酸菌+1×10⁸ CFU/kg丁酸梭菌。预试期7 d，试验期28 d。结果表明：①与A组相比，B、C、D组脾脏指数和法氏囊指数均有所提高，但差异不显著（P>0.05）。②与A组相比，B、C、D组青年鸽血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性及总抗氧能力（T-AOC）均显著提高（P<0.05），丙二醛（MDA）含量显著降低（P<0.05）；与B、C组相比，D组血清T-AOC及CAT、GSH-Px活性均显著提高（P<0.05）。③与A组相比，B、D组十二指肠内胰蛋白酶活性显著提高（P<0.05），D组脂肪酶活性显著提高（P<0.05）。④与A组相比，D组十二指肠隐窝深度显著降低（P<0.05），B组十二指肠及D组十二指肠、回肠绒毛高度/隐窝深度比值均显著提高（P<0.05）。综上所述，在日粮中添加丁酸梭菌和乳酸菌有利于改善青年鸽肠道形态，提高小肠消化酶活性及血清抗氧化能力，从而增强青年鸽的免疫力。

关键词: 益生菌; 青年鸽; 免疫性能; 抗氧化; 肠道功能

Abstract:

The study was designed to investigate the effects of Clostridium butyrate and Lactobacillus on immune performance,serum antioxidant indexes and intestinal function in young pigeons.384 female young pigeons about 80 days old were randomly divided into 4 groups with 6 replicates per group and 16 young pigeons per replicate.Among them,group A was fed with basal diet,and groups B,C and D were added 1×10⁸ CFU/kg Clostridium butyrate,5×10⁹ CFU/kg Lactobacillus,5×10⁹ CFU/kg Lactobacillus+1×10⁸ CFU/kg Clostridium butyrate in the basal diet,respectively.The preliminary trial period was 7 d and the trial period was 28 d.The results showed that:①Compared with group A,the spleen index and bursa of Fabricius index in groups B,C,D were increased but the difference was not significant (P>0.05).②Compared with group A,the serum CAT,SOD,T-AOC and GSH-Px of young pigeons in groups B,C,D were significantly increased (P<0.05),the content of MDA were significantly decreased (P<0.05);Compared with groups B,C,the serum CAT,T-AOC and GSH-Px of group D were significantly increased (P<0.05).③Compared with group A,the activity of trypsin in duodenum of groups B and D were significantly increased (P<0.05),and the activity of lipase of group D was significantly increased (P<0.05).④Compared with group A,the duodenal crypt depth in group D was significantly decreased (P<0.05),The ratio of villus height to crypt depth of duodenum in groups B and D,and that of ileum in group D were significantly increased (P<0.05).In conclusion,adding Clostridium butyrate and Lactobacillus to diets could improve the intestinal morphology,increase the activity of intestinal digestive enzymes and enhance the serum antioxidation ability of young pigeons,thereby enhance the immunity of young pigeons.

Key words: probiotics; young pigeons; immune performance; antioxidant; intestinal function

中图分类号:

S836

袁文华, 李国勤, 韩安法, 王淼, 李浙烽, 赵威, 刁新平, 卢立志. 丁酸梭菌和乳酸菌对青年鸽免疫指标、血清抗氧化指标及肠道功能的影响[J]. 《中国畜牧兽医》, 2019, 46(7): 1969-1975.

YUAN Wenhua, LI Guoqin, HAN Anfa, WANG Miao, LI Zhefeng, ZHAO Wei, DIAO Xinping, LU Lizhi. Effects of Clostridium butyrate and Lactobacillus on Immune Indexs, Serum Antioxidation Indexes and Intestinal Function in Young Pigeons[J]. , 2019, 46(7): 1969-1975.

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丁酸梭菌和乳酸菌对青年鸽免疫指标、血清抗氧化指标及肠道功能的影响

Effects of Clostridium butyrate and Lactobacillus on Immune Indexs, Serum Antioxidation Indexes and Intestinal Function in Young Pigeons

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