河南省猪链球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.02.027

摘要/Abstract

摘要：

为探讨河南省猪链球菌的血清型分布和耐药性情况，本试验对2016年5月~2017年5月从河南省猪场采集的各种组织病料进行猪链球菌的分离培养、生化鉴定及PCR鉴定，并进行了血清群的分群鉴定和血清型的分型鉴定，对其中的2型猪链球菌进行了药敏试验。结果显示，试验共鉴定出189株猪链球菌，流行的猪链球菌的血清群主要以D群为主，其次是G群和C群；优势的血清型是2型、7型、9型和1型。其中87株为2型猪链球菌，超过90%的2型菌株对β-内酰胺类（青霉素G、阿莫西林和头孢菌素类）、氯霉素、万古霉素、环丙沙星敏感，超过40%的2型菌株对多西环素、四环素、红霉素、复方新诺明、丁胺卡那霉素、克林霉素、链霉素、米诺环素产生耐药性。以上结果为指导猪场使用敏感药物及时控制疫情、选择血清型相符的疫苗进行预防及减少损失提供参考依据。

关键词: 猪链球菌; 血清型; 血清群; 耐药性

Abstract:

In order to investigate the serotype distribution and drug resistance of Streptococcus suis in Henan province. Isolation and culture,biochemical and PCR identification of suspected Streptococcus suis were performed on disease materials collected from pig farms in Henan province from May 2016 to May 2017. Identification of serum groups and serotype and the susceptibility test of Streptococcus suis serotype 2 was carried out.The results showed that 189 strains Streptococcus suis were identified,the epidemic group of Streptococcus suis in Henan province was mainly D group, followed by G and C groups; The predominant serotypes were type 2, type 7, type 9 and type 1. Among them, 87 strains were Streptococcus suis type 2. More than 90% of the isolates were susceptible to beta lactams (penicillin G, amoxicillin and cephalosporins), chloramphenicol, vancomycin and ciprofloxacin, and more than 40% strains were resistant to doxycycline, tetracycline, erythromycin, sulfamethoxazole, amikacin, clindamycin, streptomycin, minocycline resistance. These results had practical significance for guiding the use of sensitive drugs in the pig farm, controlling the epidemic situation, reducing the loss, and guiding to choose the vaccine with the same serotype.

Key words: Streptococcus suis; serotype; serogroup; drug resistance

中图分类号:

S858.28

徐引弟, 孙帅杰, 王治方, 张青娴, 朱文豪, 焦文强, 李海利, 郎利敏, 王克领. 河南省猪链球菌的分离鉴定及耐药性分析[J]. 《中国畜牧兽医》, 2018, 45(2): 501-509.

XU Yindi, SUN Shuaijie, WANG Zhifang, ZHANG Qingxian, ZHU Wenhao, JIAO Wenqiang, LI Haili, LANG Limin, WANG Keling. Isolation, Identification and Drug Resistance Analysis of Streptococcus suis in Henan Province[J]. , 2018, 45(2): 501-509.

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