东串猪Mx1基因第14外显子多态性及组织表达谱分析

doi:10.16431/j.cnki.1671-7236.2018.02.020

摘要/Abstract

摘要：

为了揭示抗黏液病毒1（myxo-virus resistance 1，Mx1）基因第14外显子在东串猪群体中多态性分布及组织表达特性，试验利用PCR-RFLP技术测定东串猪Mx1基因第14外显子的多态性，同时利用实时荧光定量PCR技术分析其组织特异性表达规律。结果表明，东串猪Mx1基因第14外显子Hin6Ⅰ酶切位点存在G36T突变，可检测到AA、AB、BB 3种基因型，AB为优势等位基因型，A为优势等位基因；东串猪Mx1基因的多态信息含量（polymorphism information content，PIC）值为0.360，表现为中度多态；Mx1基因在淋巴、肺脏、脾脏、十二指肠、空肠、回肠组织中表达量较高。本研究初步揭示了东串猪Mx1基因第14外显子的多态性分布，为探讨其作为有效遗传标记的可行性提供参考。此外，Mx1基因在多种免疫组织中高表达，可能与机体抵抗病原感染有关。

关键词: 东串猪; Mx1基因; 多态性; 表达谱

Abstract:

In this study, the PCR-RFLP and Real-time quantitative PCR assays were performed to explore the tissue expression patterns of myxo-virus resistance 1 (Mx1) gene and identify the poly-morphisms of Mx1 gene exon 14 in Dongchuan pig. The results demonstrated that there was a G36T mutation at the Hin6 Ⅰ restriction site of Mx1 gene exon 14. Furthermore, three genotypes were detected and named as AA, AB and BB, AB genotype was the dominant genotype and A was the dominant gene. Genetic polymorphism analysis showed that the polymorphism information content (PIC) value of Mx1 gene in Dongchuan pig was 0.360, which displayed moderate polymorphism. The Mx1 gene had higher expression levels in lymphatic, lung, spleen, duodenum, jejunum and ileum.This study preliminarily revealed the polymorphism distribution of Mx1 gene exon 14 in Dongchuan pig, and provided a reference for its feasibility as an effective genetic marker. In addition, the high expression of Mx1 gene in the immune tissues might be associated with the resistance to pathogenic infections.

Key words: Dongchuan pigs; Mx1 gene; polymorphisms; expression profile

中图分类号:

S813.3

吴嘉韵, 朱恩昊, 王海飞, 吴圣龙, 包文斌. 东串猪Mx1基因第14外显子多态性及组织表达谱分析[J]. 《中国畜牧兽医》, 2018, 45(2): 448-455.

WU Jiayun, ZHU Enhao, WANG Haifei, WU Shenglong, BAO Wenbin. Polymorphism and Tissue Expression Profile of Mx1 Gene Exon 14 in Dongchuan Pigs[J]. , 2018, 45(2): 448-455.

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