血根碱对大鼠离体子宫平滑肌收缩的影响及其机制研究

doi:10.16431/j.cnki.1671-7236.2017.07.044

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 2209-2213.doi: 10.16431/j.cnki.1671-7236.2017.07.044

• 基础兽医 • 上一篇

血根碱对大鼠离体子宫平滑肌收缩的影响及其机制研究

贾海燕, 徐婉晴, 张庭华, 高慧倩, 王丽芳, 王慧

临沂大学药学院, 临沂 276000

收稿日期:2016-12-30 出版日期:2017-07-20 发布日期:2017-07-22
通讯作者: 王慧 E-mail:wanghui0512@lyu.edu.cn
作者简介:贾海燕(1994-),女,山东菏泽人,学士,研究方向:中草药提取及药效,E-mail:wys450@163.com
基金资助:
国家级大学生创新创业训练计划项目（201510452025）；2016年度临沂大学教学研究与改革项目；2015年度临沂大学网络在线课程（51616006）

Effect of Sanguinarine on Active Capacity of Isolated Rat Uterine Smooth Muscle in vitro and Its Mechanism

JIA Hai-yan, XU Wan-qing, ZHANG Ting-hua, GAO Hui-qian, WANG Li-fang, WANG Hui

School of Pharmacy, Linyi University, Linyi 276000, China

Received:2016-12-30 Online:2017-07-20 Published:2017-07-22

摘要/Abstract

摘要：

试验旨在研究血根碱对大鼠离体子宫平滑肌活动的影响。分离8周龄SD雌性未孕大鼠子宫平滑肌，通过BL-420F生物信号采集系统记录子宫平滑肌收缩频率和收缩幅度，计算子宫平滑肌活力。以硫酸阿托品、盐酸普萘洛尔、盐酸苯海拉明及盐酸雷尼替丁为阻断剂，观察血根碱对子宫平滑肌M、β、H₁、H₂受体的关系，探讨血根碱对子宫平滑肌的作用机制。结果显示，血根碱组和元胡止痛片组对缩宫素所致大鼠离体子宫平滑肌收缩频率、收缩幅度和活力均有显著的抑制作用（P<0.05）；应用H₁受体阻断剂苯海拉明和H₂受体阻断剂雷尼替丁后，血根碱对子宫平滑肌抑制作用基本被阻断，而应用M受体阻断剂阿托品，β受体阻断剂普萘洛尔后，血根碱对子宫平滑肌收缩仍具有显著的抑制作用（P<0.05）。综上所述，血根碱对大鼠子宫平滑肌活力具有显著的抑制作用，可能是通过抑制H₁、H₂受体实现，与M、β受体无关。

关键词: 血根碱; 大鼠; 子宫平滑肌; 收缩

Abstract:

The study was aimed to explore the effects of the sanguinarine on activity of isolated rat uterine smooth muscle in vitro. The effect of the sanguinarine on activity of the isolated myometrium of non-pregnant Sprague-Dawley rats eight weeks old was recorded by BL-420F four channels physiological recorder. Four antagonists, atropine sulfate, ranitidine hydrochloride, propranolol hydrochloride and diphenhydramine hydrochloride were used to study their mechanism, respectively. The results showed that sanguinarine and Yuan hu painkillers markedly inhibited the frequency, amplitude and activity of uterine contractions induced by oxytocin injection (P<0.05). The inhibitory effect of sanguinarine on uterine muscle contractions was blocked after using diphenhydramine hydrochloride (H₁-receptor antagonist) and ranitidine hydrochloride (H₂-receptor antagonist). However, after using atropine sulfate (M-receptor antagonist) and propranolol hydrochloride (β-receptor antagonist), sanguinarine also significantly inhibited the contractions of rat uterine smooth muscle (P<0.05). It was concluded that the effect of sanguinarine on activity of uterine smooth muscle in rats was mainly associated with H₁ receptor or H₂ receptor but not M receptor or β receptor.

Key words: sanguinarine; rat; uterine smooth muscle; contractions

中图分类号:

S853.7

贾海燕, 徐婉晴, 张庭华, 高慧倩, 王丽芳, 王慧. 血根碱对大鼠离体子宫平滑肌收缩的影响及其机制研究[J]. 《中国畜牧兽医》, 2017, 44(7): 2209-2213.

JIA Hai-yan, XU Wan-qing, ZHANG Ting-hua, GAO Hui-qian, WANG Li-fang, WANG Hui. Effect of Sanguinarine on Active Capacity of Isolated Rat Uterine Smooth Muscle in vitro and Its Mechanism[J]. , 2017, 44(7): 2209-2213.

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血根碱对大鼠离体子宫平滑肌收缩的影响及其机制研究

Effect of Sanguinarine on Active Capacity of Isolated Rat Uterine Smooth Muscle in vitro and Its Mechanism

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