日粮能量及蛋白质水平对滩羊糖类转运载体mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2017.06.012

摘要/Abstract

摘要：

试验旨在研究日粮能量及蛋白质水平对滩羊小肠和肌肉组织中主要糖类转运载体mRNA表达的影响。选取112只健康、体重相近的滩羊（公、母各半），随机分为4组，每组4个重复，每个重复7只羊。参考肉羊饲养标准（NY/T 816—2004），分别饲喂不同能量及蛋白质水平日粮，即0.84×标准水平（Ⅰ组）、0.96×标准水平（Ⅱ组）、1.08×标准水平（Ⅲ组）和1.20×标准水平（Ⅳ组）。试验分两个阶段（29~35和36~40 kg），于每个阶段末，每个重复屠宰1只试验羊，取其小肠和肌肉组织样，运用实时荧光定量PCR技术，研究SGLT1、GLUT4、GLUT5基因mRNA的表达量变化。结果显示，在29~35 kg阶段末，Ⅲ组小肠中SGLT1基因mRNA的表达量最高，显著高于其他组（P< 0.05），在36~40 kg阶段末各组间无显著差异（P> 0.05），各组肌肉中SGLT1基因mRNA的表达量在两个阶段都随着能量及蛋白质水平的提高而增加，Ⅳ组均最高；在29~35 kg阶段末，各组间小肠中GLUT4基因mRNA表达量无显著差异（P> 0.05），而在36~40 kg阶段末，GLUT4基因mRNA的表达量随着能量及蛋白质水平的提高而增加，Ⅳ组显著高于其他组（P< 0.05），在肌肉中，Ⅳ组GLUT4基因mRNA在两个阶段末表达量均最高，均显著高于Ⅰ组（P< 0.05）；在两个试验阶段，GLUT5基因mRNA的表达量无规律性。综上所述，日粮能量及蛋白质水平会影响滩羊小肠和肌肉中SGLT1、GLUT4基因mRNA的表达量，从而影响机体对葡萄糖的消化吸收。

关键词: 滩羊; 能量; 蛋白质; 葡萄糖转运载体

Abstract:

This experiment was conducted to study the effects of the diets with different energy and protein levels on mRNA expression of glucose transporters in the small intestine and muscle of Tan sheep. A total of 112 healthy Tan sheep (half male and half female) with similar initial live weight were randomly divided into four groups with four replicates per group and seven sheep per replicate. According to the Feeding Standard of Meat-producing Sheep and Goats (NY/T 816—2004),each group was fed diet with different levels of energy and protein respectively:0.84×standard level (group Ⅰ),0.96×standard level (group Ⅱ),1.08×standard level (group Ⅲ) and 1.20×standard level (group Ⅳ). The test period were divided into two stages by body weight of sheep (29-35 and 36-40 kg). At the end of each stage,one sheep was slaughtered at each replicate,and small intestine and muscle samples were collected to study mRNA relative expression of SGLT1,GLUT4 and GLUT5 genes by Real-time PCR. The results indicated that:SGLT1 gene mRNA expression levels of group Ⅲ was significantly higher than other groups in small intestine at the end of 29-35 kg stage (P< 0.05);At the end of 36-40 kg stage,the SGLT1 gene mRNA expression levels had no significant difference among the four groups (P> 0.05).In muscle,the SGLT1 gene mRNA expression level increased with the rise of energy and protein levels at both stages ,and that of group Ⅳ were the highest.In small intestine,at the end of 29-35 kg stage,the GLUT4 gene mRNA expression levels had no significant difference among the four groups (P> 0.05);At the end of 36-40 kg stage,the GLUT4 gene mRNA expression level increased with the rise of energy and protein levels,and that of group Ⅳ were significantly higher than the other groups (P< 0.05).In muscle,the GLUT4 gene mRNA expression level were the highest in group Ⅳ at both stages,and significantly higher than group Ⅰ (P< 0.05). The GLUT5 gene mRNA expression did not show any regularity at both stages. In conclusion,diets with different levels of energy and protein could significantly affect the mRNA expression of SGLT1 and GLUT4 genes,and affect absorption of glucose in sheep.

Key words: Tan sheep; energy; protein; glucose transporters

中图分类号:

S816.4

卢艳娟, 王小芳, 曾洁, 陈玉林, 张恩平. 日粮能量及蛋白质水平对滩羊糖类转运载体mRNA表达的影响[J]. 《中国畜牧兽医》, 2017, 44(6): 1657-1665.

LU Yan-juan, WANG Xiao-fang, ZENG Jie, CHEN Yu-lin, ZHANG En-ping. Effects of Diets with Different Energy and Protein Levels on mRNA Expression of Glucose Transporters in Tan Sheep[J]. , 2017, 44(6): 1657-1665.

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