《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1108-1114.doi: 10.16431/j.cnki.1671-7236.2017.04.024

• 遗传繁育 • 上一篇    下一篇

延边黄牛ACTA1基因的SNPs检测及其与肉质性状的关联分析

田万年1, 杨翰林1, 夏广军2, 严昌国2   

  1. 1. 吉林农业科技学院动物科技学院, 吉林 132101;
    2. 延边大学农学院, 延吉 133002
  • 收稿日期:2016-10-14 出版日期:2017-04-20 发布日期:2017-05-03
  • 通讯作者: 严昌国 E-mail:ycg@ybu.edu.cn
  • 作者简介:田万年(1980-),男,吉林长春人,博士,讲师,研究方向:动物遗传育种,E-mail:wannian2000@hotmail.com
  • 基金资助:

    吉林省重点学科培育项目(吉农院合字[2015]第x030号);吉林农业科技学院种子基金项目(吉农院合字[2014]第Z07号);吉林农业科技学院大学生创新项目(吉农院合字[2015]第052号)

Polymorphism of ACTA1 Gene and Its Association with Meat Quality Traits in Yanbian Yellow Cattle

TIAN Wan-nian1, YANG Han-lin1, XIA Guang-jun2, YAN Chang-guo2   

  1. 1. College of Animal Science, Jilin Agricultural Science and Technology College, Jilin 132101, China;
    2. College of Agriculture, Yanbian University, Yanji 133002, China
  • Received:2016-10-14 Online:2017-04-20 Published:2017-05-03

摘要:

试验旨在研究延边黄牛ACTA1基因的遗传变异,分析其多态性对肉质性状的影响。采用PCR-RFLP和PCR-SSCP方法探讨ACTA1基因多态性及其与肉质性状的关联性。结果发现,延边黄牛ACTA1基因存在2个突变位点:A193G突变,导致氨基酸Arg → Gly的错义突变;A298G突变,导致氨基酸Lys →Arg的错义突变。χ2检验显示,A193G和A298G位点于检测群体中处于Hardy-Weinberg平衡状态(P>0.05)。关联分析显示,延边黄牛ACTA1基因2个突变位点的不同基因型与肌肉中肉豆蔻酸、油酸含量、眼肌面积、大理石花纹等级呈显著相关(P<0.05)。初步认为ACTA1基因对延边黄牛肉质性状有显著影响,可作为延边黄牛新品种早期选择的候选基因。

关键词: 延边黄牛; ACTA1基因; 多态性; 肉质性状

Abstract:

This study was aimed to research the gentic variations of ACTA1 gene in Yanbian Yellow cattle, and analyze the influence of genetic polymorphisms on meat quality traits. PCR-RFLP and PCR-SSCP technique were used to analyze the association between the polymorphisms of ACTA1 gene and meat quality traits. The results showed that there were two SNPs, A193G which with replacement of Arg by Gly amino acid, A298G which with replacement of Lys by Arg amino acid. χ2 test showed A193G and A298G were in Hardy-Weinberg equilibrium (P>0.05). The association analysis indicated that the genotypes of different mutations in ACTA1 gene were found to be significantly related to myristic acid, oleic acid, loin muscle area, marbling score (P<0.05). ACTA1 gene had potential effects on meat quality traits of Yanbian Yellow cattle, and could be used for candidate gene in early selection of new varieties of Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; ACTA1 gene; polymorphisms; meat quality trait

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