光周期对于绒山羊毛囊干细胞激活的影响

doi:10.16431/j.cnki.1671-7236.2017.04.008

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 994-1000.doi: 10.16431/j.cnki.1671-7236.2017.04.008

光周期对于绒山羊毛囊干细胞激活的影响

刘斌^1,2, 郭俊^1,3, 勿都巴拉^1,3, 马跃军¹, 辛雷勇⁴, 赵存发¹, 李玉荣¹, 尹俊³

1. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
2. 中国科学院昆明动物研究所, 昆明 650223;
3. 内蒙古农业大学, 呼和浩特 010018;
4. 鄂托克前旗北极神绒牧业研究所, 鄂尔多斯 016200

收稿日期:2016-09-05 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 李玉荣, 尹俊 E-mail:yurong622003@126.com;yinjunparis@163.com
作者简介:刘斌(1979-),男,甘肃陇西人,博士,副研究员,研究方向:绒毛用羊分子数量遗传育种,E-mail:liubin0613@126.com;郭俊(1986-),男,内蒙古呼和浩特人,博士,研究方向:绒毛用羊分子数量遗传育种;E-mail:guojun_86x@163.com刘斌与郭俊对本文具有同等贡献,并列第一作者
基金资助:
国家自然科学基金项目（31260545）；国家公益性行业（农业）科研专项（201303059）；内蒙古自治区自然科学基金项目（2014BS0333）；内蒙古自治区创新基金项目（2013CXJJM03）；内蒙古农牧业科学院青年创新基金项目（2013QNJJM05）；内蒙古自然科学基金重大项目（2013ZD06）

Effects of Photoperiod on Stem Cells Activation in Cashmere Goat Hair Follicles

LIU Bin^1,2, GUO Jun^1,3, Wudubala^1,3, MA Yue-jun¹, XIN Lei-yong⁴, ZHAO Cun-fa¹, LI Yu-rong¹, YIN Jun³

1. Inner Mongolia Academy of Agriculture & Animal Husbandry Sciences, Hohhot 010031, China;
2. Kunming Institute of Animal Science, Chinese Academy of Sciences, Kunming 650223, China;
3. Inner Mongolia Agricultural University, Hohhot 010018, China;
4. Etuokeqianqi Arctic God Research Institute of Cashmere and Livestock, Erdos 016200, China

Received:2016-09-05 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

为了延长绒山羊毛囊兴盛期，提高羊绒产量，本试验通过人工缩短内蒙古阿尔巴斯型绒山羊的日照时间，利用组织切片技术对比人工短光照和自然光照周期下绒山羊皮肤组织形态变化，利用免疫组织化学方法研究毛囊细胞增殖及相关信号通路重要蛋白的表达。结果显示，人工短光照周期下绒山羊毛囊细胞增殖强烈，干细胞标记角蛋白15（cytokeratin 15，K15）阳性信号大量分布于外根鞘，β-catenin信号活跃表达于毛母质及根鞘，毛囊提前进入兴盛期；而自然光照周期下绒山羊毛囊仍停留在休止期，相应蛋白信号表达较弱。综上表明，短日照对毛囊生长具有明显的促进作用，人工短光照周期可以提前激活毛囊干细胞，使其提前进入兴盛期，引起多种毛囊生长相关蛋白的表达。

关键词: 绒山羊; 短日照; 细胞增殖; 干细胞; 生长调控

Abstract:

In order to extend the anagen of cashmere goat hair follicles and increase the production of cashmere,this study was performed with artificially shorten the daylight time among Arbas White cashmere goats. Skin tissue sections from cashmere goats were collected to compare the morphologic changes between artificial daylight and natural daylight,and immunohistochemical method was used to study the hair follicle cell proliferation and important protein expression in related signaling pathways. The results showed that strong cell proliferation occurred in cashmere goat hair follicle cells during artificial daylight,plenty of cytokeratin 15 (K15) positive signals were distributed in the outer root sheath,β-catenin protein was actively expressed in hair matrix and root sheath, indicating that the hair follicles were in the anagen growth phase;Meanwhile,cashmere goat hair follicles under natural daylight were in telogen with weak signals. Above all prove that short photoperiod played an important role in promoting hair follicle growth,the artificial short photoperiod could change hair follicle growth cycle and make hair follicles earlier enter to the anagen growth phase,causing a variety of typical gene expressions during hair follicle growth.

Key words: cashmere goat; short photoperiod; cell proliferation; stem cells; growth regulation

中图分类号:

S826.9⁺1

刘斌, 郭俊, 勿都巴拉, 马跃军, 辛雷勇, 赵存发, 李玉荣, 尹俊. 光周期对于绒山羊毛囊干细胞激活的影响[J]. 《中国畜牧兽医》, 2017, 44(4): 994-1000.

LIU Bin, GUO Jun, Wudubala, MA Yue-jun, XIN Lei-yong, ZHAO Cun-fa, LI Yu-rong, YIN Jun. Effects of Photoperiod on Stem Cells Activation in Cashmere Goat Hair Follicles[J]. , 2017, 44(4): 994-1000.

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光周期对于绒山羊毛囊干细胞激活的影响

Effects of Photoperiod on Stem Cells Activation in Cashmere Goat Hair Follicles

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