猪多潜能干细胞系的研究进展

doi:10.16431/j.cnki.1671-7236.2023.01.007

摘要/Abstract

摘要： 猪多潜能干细胞是在体外建立起来的具有自我更新及三胚层分化潜能的一类干细胞，可应用于发育生物学研究、基因组编辑和疾病模型建立等各方面，在畜牧业生产及再生医学研究中具有重要的应用价值。培养体系对多潜能干细胞的成功构建具有重要意义，其包含多种成分，包括基础培养液、氨基酸等营养成分、小分子化合物（信号通路激活剂/抑制剂及细胞因子）共同维持细胞的多能性并抑制其分化。目前已有诸多关于猪多潜能干细胞的报道，但尚未获得高效的培养体系可以维持猪多潜能干细胞的长期传代并完成生殖嵌合。猪多潜能干细胞可分为原始态（Naïve）、形成态（Formative）及始发态（Primed）3种不同多能性的状态特点，根据建系来源的不同分为猪扩展多潜能干细胞、猪胚胎干细胞、猪前原肠胚上胚层干细胞、猪诱导多能干细胞4种干细胞类型。作者综述了猪多潜能干细胞培养体系中常用的细胞因子和信号通路激活剂/抑制剂对猪多潜能干细胞的多能性和分化能力的影响和作用，为进一步建立具有真正生殖嵌合能力的Naïve多能性的猪多潜能干细胞系提供研究思路。

关键词: 猪; 多潜能干细胞系; 多能性

Abstract: Pig pluripotent stem cells are a kind of stem cells established in vitro with the capability of self-renewal and three germ layers differentiation, which can be used in developmental biology research, genome editing and disease modeling establishment.It has great value of application in animal husbandry production and regenerative medicine research.The culture system is of great significance for the successful construction of pluripotent stem cells, which contains a variety of components, including basic culture medium, amino acids and other nutrients, small molecular compounds (cytokines and signal pathway activators or inhibitors) to maintain cell pluripotency and inhibit its differentiation.There are many previous reports about porcine pluripotent stem cells, but effective culture system has not been obtained to maintain the long-term passage of porcine pluripotent stem cells and complete germline chimerism.Porcine pluripotent stem cells can be divided into three pluripotent states:Naïve state, Formative state and Primed state.And they can be classified into porcine expanded pluripotent stem cells, porcine embryonic stem cells, porcine pregastrulation stem cells and porcine induced pluripotent stem cells according to the derivation of the cell lines.Overall, the authors summarized the functional influences of current application of cytokines and signal pathway activators or inhibitors on the pluripotency and differentiation ability of porcine pluripotent stem cells.The present review could provide guidlines for the establishment of Naïve pluripotency porcine pluripotent stem cells with germline chimerism.

Key words: porcine; pluripotent stem cell lines; pluripotency

中图分类号:

S828

王志龙, 刘丽, 张鲁, 高帅. 猪多潜能干细胞系的研究进展[J]. 中国畜牧兽医, 2023, 50(1): 67-75.

WANG Zhilong, LIU Li, ZHANG Lu, GAO Shuai. Research Progress of Porcine Pluripotent Stem Cell Lines[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 67-75.

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