连花柴芩可溶性粉对鸡风热犯肺证的临床试验研究

doi:10.16431/j.cnki.1671-7236.2022.09.035

摘要/Abstract

摘要： 【目的】研究连花柴芩可溶性粉对风热犯肺所导致的鸡发热、咳嗽、食欲减退、口渴喜饮等证候的临床疗效。【方法】采用鸡传染性支气管炎病毒人工攻毒建立风热犯肺证肉鸡病例模型，将出现典型风热犯肺证临床症状的150只鸡纳入到试验中，并随机分为5组，30只/组，分别为连花柴芩可溶性粉高、中、低剂量组（连花柴芩可溶性粉浓度分别为4、2、1 g/L），麻杏石甘颗粒药物对照组（1 g/L），模型对照组，另设30只为健康对照组。除健康对照组和模型对照组外，各试验组连续饮水给药5 d，记录给药前后鸡的精神状态、体温、呼吸道症状、采食及饮水的变化，并检测各组鸡的免疫器官指数、外周血免疫细胞亚群指标，综合考察连花柴芩可溶性粉的临床疗效。【结果】连花柴芩可溶性粉高、中剂量组对风热犯肺所致的鸡发热、咳嗽的总有效率分别为90.00%和86.66%，显著高于模型对照组（P<0.05），且对病鸡采食量下降、饮水量增多具有较好的缓解效果（P<0.05）；连花柴芩可溶性粉能提高病鸡免疫器官指数，促进病鸡外周血中CD3⁺、CD4⁺、CD8⁺ T淋巴细胞增殖，且连花柴芩可溶性粉高、中剂量显著高于模型对照组（P<0.05）。【结论】连花柴芩可溶性粉对风热犯肺所致的鸡发热、咳嗽、食欲减退、口渴喜饮具有较好的缓解和治疗效果，结合药物量效关系和经济成本等因素综合考虑，推荐连花柴芩可溶性粉临床应用剂量为2 g/L，饮水给药，连用5 d。

关键词: 连花柴芩可溶性粉; 鸡风热犯肺证; 临床疗效; 淋巴细胞增殖

Abstract: 【Objective】 This experiment was aimed to study the clinical effect of Lianhuachaiqin soluble powder on fever, cough, anorexia, thirst and drinking in chickens caused by wind-heat invading lung.【Method】 The broiler model of wind-heat invading lung syndrome was established by artificial challenge of chicken Infectious bronchitis virus.150 chickens with typical clinical symptoms of wind-heat invading lung syndrome were included in the experiment, and were randomly divided into 5 groups:Lianhuachaiqin soluble powder high, medium, low dose groups (Lianhuachaiqin soluble powder concentrations were 4, 2, 1 g/L, respectively), Ma Xing Shigan granule control group (1 g/L), and model control group, 30 chickens in each group.Another 30 chickens were used as healthy control group.Except for the healthy control group and the model group, the experimental groups were treated with drinking water continuously for 5 days.The changes of mental state, body temperature, respiratory symptoms, intake and drinking water of chickens before and after administration were recorded, and the indexes of immune organs and immune cell subsets in peripheral blood of chickens in each group were detected to comprehensively investigate the clinical effect of Lianhuachaiqin soluble powder.【Result】 The total effective rates of high and medium dose groups of Lianhuachaiqin soluble powder on fever and cough caused by wind-heat invading lung were 90.00% and 86.66% respectively, which were significantly higher than those in the model control group (P<0.05).And it had a good alleviating effect on the decrease of feed intake and the increase of drinking water in diseased chickens (P<0.05).On the other hand, the Lianhuachaiqin soluble powder could increase the immune organ index and promote the proliferation of CD3⁺, CD4⁺ and CD8⁺ T lymphocytes in the peripheral blood of diseased chickens, and the high and medium doses were significantly higher than those in the model control group (P<0.05).【Conclusion】 The results showed that Lianhuachaiqin soluble powder had good relieving and therapeutic effect on chicken fever, cough, loss of appetite and thirst caused by wind-heat invading lung. Combined with drug dose-effect relationship and economic cost, it was recommended that the clinical dosage of Lianhuachaiqin soluble powder was 2 g/L, given in drinking water for 5 days.

Key words: Lianhuachaiqin soluble powder; chicken syndrome of wind-heat invading lung; clinical efficacy; lymphocyte proliferation

中图分类号:

S853.74

王学方, 归荣, 陈功义, 王伟, 张红英, 王学兵, 李晓. 连花柴芩可溶性粉对鸡风热犯肺证的临床试验研究[J]. 中国畜牧兽医, 2022, 49(9): 3610-3621.

WANG Xuefang, GUI Rong, CHEN Gongyi, WANG Wei, ZHANG Hongying, WANG Xuebing, LI Xiao. Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3610-3621.

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