柏芩提取液的药效学及对奶牛子宫内膜炎的疗效评价

doi:10.16431/j.cnki.1671-7236.2020.05.037

摘要/Abstract

摘要： 柏芩提取液是由黄柏、黄芩、红花、党参等组成，为了探讨其对奶牛子宫内膜炎的治疗作用，采用实验性家兔子宫内膜炎模型进行柏芩提取液的主要药效学研究。确定药效作用后，按照农业农村部颁布的《防治奶牛临床子宫内膜炎的抗微生物兽药的靶动物安全性和有效性试验指导原则》对其进行奶牛子宫内膜炎的临床疗效评价。药效学试验结果表明，与模型组比较，柏芩提取液能明显改善实验性子宫内膜炎家兔的临床症状，血液白细胞计数分类、子宫分泌物涂片镜检、尿液及子宫分泌物化学检查结果趋于正常，并且能够减轻子宫充血、炎性细胞浸润、上皮细胞脱落等组织病理学改变。临床疗效试验结果表明，给药后第10天，柏芩提取液高、中剂量组的治愈率极显著高于低剂量组（P<0.01），且与阳性药物对照组无显著差异（P>0.05）；各试验组的总有效率差异均不显著（P>0.05）。给药后第21天，柏芩提取液高、中剂量组的治愈率显著高于低剂量组（P<0.05），且与阳性药物对照组差异不显著（P>0.05）；各试验组的总有效率未见明显差异（P>0.05）。以上结果证明柏芩提取液对实验性家兔子宫内膜炎和临床奶牛子宫内膜炎具有良好的治疗作用，其中1.5和1.0 g/mL浓度（30 mL/次）对临床病例的治疗效果明显，与土霉素效果相似，0.5 g/mL浓度效果不明显。本研究为其进一步研究和开发应用奠定了基础。

关键词: 柏芩提取液; 子宫内膜炎; 抗炎作用; 药效学; 临床疗效

Abstract: Baiqin extract is composed of Phellodendron chinense,Scutellaria baicalensis,Carthamus tinctorius,Codonopsis pilosula,etc.In order to investigate its therapeutic effect against cow endometritis,the main pharmacodynamics was studied by using experimental rabbit model of endometritis.Besides,the clinical efficacy was evaluated according to the 'Guideling principles for the safety and efficacy test of antimicrobial veterinary drugs for the prevention and treatment of clinical endometritis in dairy cattle' which was issued by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.The results of the pharmacodynamic experiments revealed that Baiqin extract could obviously improve the clinical symptoms of experimental endometritis in rabbits as compared to model group.It has been examined that differential leukocyte counting,uterus secretion smear microscopy,chemical examination of urine and uterus secretion were returned to normal,and abnormal histopathological changes of uterus such as hyperemia,inflammatory cells infiltrates and shedding of epithelial cells were partially alleviated in the uterus of rabbits.Clinical efficacy results showed that at 10th day after administration,the curative rates against endometritis in the high- and medium-dose groups of Baiqin extract were extremely significantly higher than that in the low-dose group (P<0.01),and were no significant difference compared with the positive drug group (P>0.05);While there was no significant difference in the total effective rates among all the tested groups (P>0.05).On 21st day post-treatment,the curative rates in the high- and medium-dose groups of Baiqin extract were significantly higher than that in the low-dose group (P<0.05),and were no significant difference compared with the positive drug group (P>0.05);While there was no significant difference in the total effective rates among all the tested groups (P>0.05).These results indicated that Baiqin extract had a good therapeutic effect on rabbit experimental endometritis and cow clinical endometritis,and the therapeutic efficacy of 1.5 or 1.0 g/mL concentration (30 mL at a time) on clinical cases was excellent,and similar with that of oxytetracycline,the therapeutic efficacy of 0.5 g/mL was non-obvious.The study would lay a foundation for further research and development.

Key words: Baiqin extract; endometritis; anti-inflammatory action; pharmacodynamics; clinical efficacy

中图分类号:

S858.31

钱峰, 苗钰松, MUHAMMAD Ishfaq, 尹鹏, 尹建丽, 李继昌. 柏芩提取液的药效学及对奶牛子宫内膜炎的疗效评价[J]. 中国畜牧兽医, 2020, 47(5): 1618-1627.

QIAN Feng, MIAO Yusong, MUHAMMAD Ishfaq, YIN Peng, YIN Jianli, LI Jichang. Pharmacodynamics of Baiqin Extract and Clinical Efficacy Evaluation for Treatment of Cow Endometritis[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1618-1627.

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