华南虎子宫内膜炎摩氏摩根菌分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2023.03.041

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (3): 1271-1278.doi: 10.16431/j.cnki.1671-7236.2023.03.041

华南虎子宫内膜炎摩氏摩根菌分离鉴定及生物学特性研究

胡依凡¹, 马敬华², 白思琦³, 翟祎梦¹, 刘建勋², 宋厚辉¹, 杨永春¹

1. 浙江农林大学动物科技学院·动物医学院, 浙江省畜禽绿色生态健康养殖应用技术研究重点实验室, 动物健康互联网检测技术浙江省工程研究中心, 浙江省动物医学与健康管理国际科技合作基地, 中澳动物健康大数据分析联合实验室, 杭州 311300;
2. 杭州野生动物世界, 杭州 311400;
3. 山东畜牧兽医职业学院, 潍坊 261000

收稿日期:2022-09-09 出版日期:2023-03-05 发布日期:2023-03-02
通讯作者: 宋厚辉, 杨永春 E-mail:songhh@zafu.edu.cn;yyc@zafu.edu.cn
作者简介:胡依凡,E-mail:yifan092@gmail.com;马敬华,E-mail:313699382@qq.com。
基金资助:
浙江省重点研发计划项目(2019C02052);国家自然科学基金(32102671);国家级大学生创新创业训练计划项目(202210341047);浙江农林大学2021年校级学生科研训练项目(2021KX0210)

Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis)

HU Yifan¹, MA Jinghua², BAI Siqi³, ZHAI Yimeng¹, LIU Jianxun², SONG Houhui¹, YANG Yongchun¹

1. Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China;
2. Hangzhou Safari Park, Hangzhou 311400, China;
3. Shandong Vocational Animal Science and Veterinary College, Weifang 261000, China

Received:2022-09-09 Online:2023-03-05 Published:2023-03-02

摘要/Abstract

摘要： 【目的】对杭州野生动物世界1例成年雌性华南虎(Panthera tigris amoyensis)子宫内膜炎病例的病原进行分离鉴定及生物学特性研究。【方法】无菌采集患虎子宫分泌物进行细菌分离培养、形态观察、梅里埃Vitek^® 2 COMPACT系统的生化鉴定以及16S rDNA序列分析,采用小鼠腹腔接种模型检测临床分离株致病性,并通过药敏纸片扩散法K-B鉴定该菌对50种药物的敏感性,采用筛选的敏感药物联合对症治疗,持续观察疾病是否复发及其产仔情况。【结果】从患虎子宫分泌物中获得1株革兰阴性短棒状杆菌;梅里埃Vitek^® 2 COMPACT系统革兰阴性卡鉴定结果显示,该临床分离株为摩氏摩根菌,菌株可发酵葡萄糖,分解甘露糖、D-葡萄糖,且尿素酶、磷酸酶、鸟氨酸脱羧酶、γ-谷氨酰转移酶反应呈阳性;序列比对分析发现,16S rDNA序列与摩氏摩根菌的相似性最高,达99%以上,进一步表明该菌为摩氏摩根菌,并命名为HNH2019;药敏结果显示,该菌多重耐药,对多黏菌素B、丁胺卡那、复方新诺明、麦迪霉素、四环素、头孢哌酮等33种药物呈不同程度的耐药,对头孢噻肟、头孢曲松和头孢西丁等6种药物中度敏感,对妥布霉素、洛美沙星、依诺沙星和氟苯尼考等11种药物敏感;小鼠致病性分析结果显示,该分离株腹腔接种ICR小鼠的半数致死量为1×10^7.5 CFU;采用敏感药物治疗后,该虎痊愈且连续3年未复发,截至2022年9月,该母虎已经多次怀孕并合计生产了5仔。【结论】本研究分离鉴定到1株华南虎源多重耐药致病性摩氏摩根菌,经筛选的敏感药物治疗,该虎子宫内膜炎痊愈未复发,研究结果为同类疫病防治提供了病例参考和数据支持,提示应重视摩氏摩根菌对动物的潜在危害。

关键词: 华南虎; 摩氏摩根菌; 子宫内膜炎; 耐药

Abstract: 【Objective】 The pathogen of a case of endometritis of an adult female South China tiger (Panthera tigris amoyensis) in Hangzhou Safari Park was isolated and identified,and its biological characteristics were studied.【Method】 The uterine secretions of the tiger were collected aseptically and the pathogenic bacteria were identified by using bacterial isolation and purification,morphological observation,biochemical identification of Merier Virtek^® 2 COMPACT system,and 16S rDNA sequence analysis.The pathogenicity of the clinical isolate was detected by intraperitoneal inoculation model of mice,and the sensitivity of the isolates to 50 drugs was identified by the universal Kirby-Bauer (K-B) test.The selected sensitive drugs were combined with symptomatic treatment to continuously observe whether the disease recurred and the birth situation.【Result】 A Gram-negative short rod-shaped bacillus isolate was obtained from the uterine secretion of the tiger.The identification result of Merrier Vitek^® 2 COMPACT system’s Gram negative card showed that the clinical isolated strain was Morganella morganii,which could ferment glucose,decompose mannose,D-glucose and urease,phosphatase,ornithine decarboxylase γ-glutamyltransferase reaction was positive.The sequence comparison analysis showed that the 16S rDNA sequence had the highest similarity with Morganella morganii,more than 99%.The results further showed that the bacterium was Morganella morganii,and was named HNH2019.The drug sensitivity results showed that the bacterium was multi resistant to 33 drugs,including polymyxin B,amikacin,cotrimoxazole,medimycin,tetracycline and cefoperazone,moderately sensitive to 6 drugs,including cefotaxime,ceftriaxone and cefoxitin,and sensitive to 11 drugs,including tobramycin,lomefloxacin,enoxacin and florfenicol.The results of mouse pathogenicity analysis showed that the median lethal dose of the isolate inoculated intraperitoneally in ICR mice was 1×10^7.5 CFU.After treatment with sensitive drugs,the tiger recovered and did not recur for three consecutive years.By September 2022,the female tiger had been pregnant many times and had given birth to five cubs in total.【Conclusion】 In this study,a Morganella morganii isolate was proposed as a potential pathogen of the South China tiger.After treatment with sensitive drugs selected by drug sensitivity test,the tiger recovered from endometritis without recurrence.The results of the study provided case reference and data support for the prevention and control of similar diseases,suggesting that attention should be paid to the potential harm of Morganella morganii to animals.

Key words: South China tiger (Panthera tigris amoyensis); Morganella morganii; endometritis; drug resistance

中图分类号:

S852.61⁺2

胡依凡, 马敬华, 白思琦, 翟祎梦, 刘建勋, 宋厚辉, 杨永春. 华南虎子宫内膜炎摩氏摩根菌分离鉴定及生物学特性研究[J]. 中国畜牧兽医, 2023, 50(3): 1271-1278.

HU Yifan, MA Jinghua, BAI Siqi, ZHAI Yimeng, LIU Jianxun, SONG Houhui, YANG Yongchun. Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis)[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1271-1278.

参考文献

[1] 范克伟,余佳,杨守深,等.华南虎源奇异变形杆菌的分离鉴定及其系统发育分析[J].中国兽医科学,2018,48(1):69-75. FAN K W,YU J,YANG S S,et al.Isolation,identification and phylogenetic analysis of Proteus mirabilis from South China tiger[J].Chinese Veterinary Science,2018,48(1):69-75.(in Chinese)
[2] 范克伟,余佳,杨守深,等.华南虎源禽致病性大肠杆菌的分离鉴定及其生物学特性研究[J].西北农林科技大学学报(自然科学版),2018,46(12):1-10. FAN K W,YU J,YANG S S,et al.Isolation,identification and biological characteristics of avian pathogenic Escherichia coli from South China tiger[J].Journal of Northwest A&F University(Natural Science Edition),2018,46(12):1-10.(in Chinese)
[3] 谢和平,朱庆艳,陈武,等.华南虎源大肠杆菌的快速鉴定及致病特性的初步研究[J].野生动物,2012,33(3):109-112. XIE H P,ZHU Q Y,CHEN W,et al.Rapid identification of an Escherichia coli isolated from South China tiger (Panthera tigris amoyensis) and preliminary study of pathogenic characteristics[J].Chinese Journal of Wildlife,2012,33(3):109-112.(in Chinese)
[4] 韦阳道,易弋,黎娅,等.胡子鲶致病性摩氏摩根菌的分离鉴定与致病性研究[J].中国畜牧兽医,2017,44(1):221-226. WEI Y D,YI Y,LI Y,et al.Isolation,identification and pathogenicity analysis of Morganella morganii in Clarias fuscus[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):221-226.(in Chinese)
[5] 张晨琪,韦微,阙腾程,等.感染摩氏摩根菌穿山甲的收容救护及其致病性分析[J].蛇志,2019,31(1):16-18. ZHANG C Q,WEI W,QUE T C,et al.Rescue the pangolins infected with Morganella morganii and analysis of its pathogenicity[J].Journal of Snake (Science & Nature),2019,31(1):16-18.(in Chinese)
[6] 赵耘,李伟杰,杜昕波,等.袋鼠摩根氏菌生物特性鉴定及系统发育分析[J].中国畜牧兽医,2010,37(3):48-51. ZHAO Y,LI W J,DU X B,et al.Phylogenetic analysis and identification of biological characterization of Morganella morganii in kangaroo[J].China Animal Husbandry & Veterinary Medicine,2010,37(3):48-51.(in Chinese)
[7] LI G,NIU X,YUAN S,et al.Emergence of Morganella morganii subsp.morganii in dairy calves,China[J]. Emerging Microbes & Infections,2018,7(1):172.
[8] FALAGAS M E,KAVVADIA P K,MANTADAKIS E,et al.Morganella morganii infections in a general tertiary hospital[J]. Infection,2006,34(6):315-321.
[9] KIM J H,CHO C R,UM T H,et al.Morganella morganii sepsis with massive hemolysis[J].Journal of Korean Medical Science,2007,22(6):1082-1084.
[10] LIU H,ZHU J,HU Q,et al.Morganella morganii,a non-negligent opportunistic pathogen[J]. International Journal of Infectious Diseases,2016,50:10-17.
[11] NICOLLE L E.Urinary tract pathogens in complicated infection and in elderly individuals[J].Journal of Infectious Diseases,2001,183(Suppl 1):S5-S8.
[12] 许赞焕,罗琳,姜娜,等.鼋摩氏摩根氏菌的鉴定及致病性[J].四川农业大学学报,2012,30(1):87-91. XU Z H,LUO L,JIANG N,et al.Isolation and identification of pathogenic Morganella morganii isolated from infected soft-shelled turtle (Pelochelys bibroni)[J].Journal of Sichuan Agricultural University,2012,30(1):87-91.(in Chinese)
[13] 孔蕾,朱凝瑜,贝亦江,等.中华鳖(Pelodiscus sinensis)摩氏摩根菌(Morganella morganii)的鉴定及致病性研究[J]. 海洋与湖沼,2013,44(3):722-727. KONG L,ZHU N Y,BEI Y J,et al.Isolation and identification of pathogenic Morganella morganii isolates from Chinese soft-shelled turtle Pelodiscus sinensis[J].Oceanologia et Limnologia Sinica,2013,44(3):722-727.(in Chinese)
[14] ELFADL A K,LEE S W,KIM J H,et al.Fatal fibrino-hemorrhagic bronchopneumonia associated with Morganella morganii in a bottlenose dolphin:A case report[J]. Diseases of Aquatic Organisms,2017,127(1):41-47.
[15] 王磊,孙培英,陈再忠,等.黑格尔七彩神仙鱼摩氏摩根菌分离鉴定及耐药性分析[J]. 南方农业学报,2016,47(10):1772-1777. WANG L,SUN P Y,CHEN Z Z,et al.Isolation,identification and drug resistance analysis of Morganella morganii from Symphysodon discus[J].Journal of Southern Agriculture,2016,47(10):1772-1777.(in Chinese)
[16] 李雪峰,王利.鲈鱼摩氏摩根菌的鉴定及药敏试验[J].动物医学进展,2015,36(2):65-68. LI X F,WANG L.Identification and drug sensitivity test of Morganella morganii isolated from Lateolabrax japonicus[J].Progress in Veterinary Medicine,2015,36(2):65-68.(in Chinese)
[17] GREISEN K,LOEFFELHOLZ M,PUROHIT A,et al.PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria,including bacteria found in cerebrospinal fluid[J].Journal of Clinical Microbiology,1994,32(2):335-351.
[18] LIU H,YAN Q,ZHAO B,et al.Isolation,molecular characterization,and phylogenetic analysis of Encephalomyocarditis virus from South China tigers in China[J]. Infection Genetics and Evolution,2013,19:240-243.
[19] SAKAGUCHI S,NISHI K,YAMASHITA Y,et al.White urine due to urinary tract infection[J]. Kidney International,2014,86(3):655.
[20] MURPHY K,RYAN C,DEMPSEY E M,et al.Neonatal sulfhemoglobinemia and hemolytic anemia associated with intestinal Morganella morganii[J]. Pediatrics,2015,136(6):e1641-e1645.
[21] LIN T Y,CHAN M C,YANG Y S,et al.Clinical manifestations and prognostic factors of Morganella morganii bacteremia[J]. European Journal of Clinical Microbiology & Infectious Diseases,2015,34(2):231-236.
[22] SHAHBAZE E,MOLLASALHI H,MINAI-TEHRANI D.Development and evaluation of an improved quantitative loop-mediated isothermal amplification method for rapid detection of Morganella morganii[J].Talanta,2019,191:54-58.
[23] 陈永亮,高晓华,李怡,等.鳖源摩氏摩根菌分离鉴定及药敏特性分析[J].南方农业学报,2015,46(11):2046-2052. CHEN Y L,GAO X H,LI Y,et al.Isolation,identification and antibiotic sensitivity test of Morganella morganii from Trionyx sinensis[J].Journal of Southern Agriculture,2015,46(11):2046-2052.(in Chinese)
[24] ROJAS L,VINUESA T,TUBAU F,et al.Integron presence in a multiresistant Morganella morganii isolate[J]. International Journal of Antimicrobial Agents,2006,27(6):505-512.

华南虎子宫内膜炎摩氏摩根菌分离鉴定及生物学特性研究

Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis)

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈建师, 张宁, 浦方雄, 苗健佳, 曾楠, 李炳学. 炭疽芽孢杆菌携带前噬菌体的预测及耐药性与毒力分析[J]. 中国畜牧兽医, 2023, 50(6): 2573-2584.
[2]	曾成容, 刘馨, 毕文文, 梅世慧, 何广霞, 张俊杰, 文明, 周碧君, 陈江凤, 姜海波. 嗜水气单胞菌群体感应系统及其抑制剂应用研究进展[J]. 中国畜牧兽医, 2023, 50(5): 1991-2000.
[3]	邵永斌, 郑一闯, 张竞怡, 魏琳颖, 田勇, 孙国结, 刘雨然, 罗燕, 谷新利. 益母草碱对LPS诱导小鼠子宫内膜炎的抗炎作用研究[J]. 中国畜牧兽医, 2023, 50(5): 2149-2155.
[4]	刘鑫煜, 崔强, 张兰欣, 范佺荣, 李政忆, 高瑞峰. 基于网络药理学和分子对接探究诃子鞣酸防治奶牛子宫内膜炎的作用机制[J]. 中国畜牧兽医, 2023, 50(4): 1632-1641.
[5]	李翠函, 曲志娜, 高玉斌, 李彦, 王娟, 段笑笑, 王琳, 张喜悦, 赵格, 黄秀梅, 赵建梅, 张青青, 王君玮, 黄保续, 刘俊辉. 三类禽源碳青霉烯类耐药大肠杆菌流行病学调查及全基因组测序分析[J]. 中国畜牧兽医, 2023, 50(4): 1653-1662.
[6]	王路才, 撒朗文朱, 张焕容. 禽源肠炎沙门菌的分离鉴定及耐药性与致病性分析[J]. 中国畜牧兽医, 2023, 50(4): 1663-1674.
[7]	吴植, 张聪, 袁慧莎, 董洪燕, 戴利霞, 贾鲁起, 吴双, 朱善元. 黄鸡源沙门菌分离鉴定及毒力和耐药性分析[J]. 中国畜牧兽医, 2023, 50(4): 1695-1706.
[8]	曹颖颖, 梁亮, 曾怡蓉, 钟清华, 袁小芳, 唐海波, 冷静. 滇西树鼩源沙门氏菌的分离鉴定及生物学特性研究[J]. 中国畜牧兽医, 2023, 50(4): 1718-1728.
[9]	朱琪琪, 张继瑜, 王承业, 吴灵玉, 王玮玮, 朱阵. 河北地区动物源大肠杆菌多重耐药性与流行性分析[J]. 中国畜牧兽医, 2023, 50(3): 1081-1092.
[10]	祝希辉, 李艳兰, 王志伟, 庞喆羽, 李玉保, 刘成, 司振书, 路建彪, 曹胜亮, 薛希娟. 1株鸭疫里默氏杆菌的全基因组测序及耐药性与遗传进化分析[J]. 中国畜牧兽医, 2023, 50(3): 1140-1149.
[11]	田二杰, 谷振, 王子皓, 刘方正, 刘克森, 张雪飞, 周变华. 黄芩对安普霉素耐药大肠杆菌的耐药消除研究[J]. 中国畜牧兽医, 2023, 50(3): 1279-1286.
[12]	王冰艺, 马弘财, 邹明昊, 樊世杰, 元振杰, 拉巴次仁, 董海龙, 曾江勇. 牦牛源链球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 745-753.
[13]	马晓姣, 赵艳坤, 邵伟, 武亚婷, 李明, 刘慧敏, 孟璐, 陈贺. 新疆部分地区生乳中金黄色葡萄球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 789-797.
[14]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[15]	刘琳, 吴保庆, 郭晓银, 于文会, 田浪. 石榴皮水提物对猪源多重耐药大肠杆菌的抑菌作用研究[J]. 中国畜牧兽医, 2023, 50(1): 328-340.