紫苏籽活性成分的抗炎作用及其在畜牧生产中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2019.01.014

摘要/Abstract

摘要：

紫苏籽是一味药食两用的传统中药材，具有解热镇痛，抑菌消炎等功效。紫苏籽富含α-亚麻酸，而α-亚麻酸有助于维持免疫细胞正常的结构与功能，并能抑制炎症因子的产生；紫苏醛既能抑制促炎因子基因的表达，又可提升动物自身的免疫能力；黄酮及酚酸类等活性物质能减少炎症细胞浸润和水肿形成，诱导病变细胞凋亡，还具有抗氧化等作用。文章主要介绍了紫苏籽中α-亚麻酸、紫苏醛、异紫苏酮、木犀草素和迷迭香酸等主要抗炎活性成分及抗炎作用，综述了紫苏籽在畜牧生产中的研究进展，介绍了紫苏籽在动物肠道菌群、生产性能、免疫功能、肉品质等方面的调节作用。此外，还探讨了不同品种紫苏籽间成分含量差异大、部分品种的毒副作用强等问题，旨在为紫苏籽资源的开发利用提供理论参考。

关键词: 紫苏籽; 紫苏醛; 畜牧生产; 抗炎作用

Abstract:

Perilla seed is a traditional Chinese medicine with antipyretic,analgesic and anti-inflammatory effects.It is rich with α-linolenic acid which is involved in maintaining the normal structure and function of immune cells and inhibiting the production of inflammatory factors.Perillaldehyde can inhibit the expression of pro-inflammatory factor genes and enhance immunity capability of animals.Flavonoids,phenolic acids and other active substances can reduce infiltration of inflammatory cell and the formation of edema,induce apoptosis of diseased cells,and also have antioxidant effects.This paper mainly introduces the main anti-inflammatory active ingredients and anti-inflammatory effects of α-linolenic acid,perillaldehyde,iso-perillaldehyde,luteolin and rosmarinic acid in Perilla seed.The research progress of its application in livestock production was reviewed,and the regulation of Perilla seed in animal intestinal flora,production performance,immune function and meat quality was analyzed.In addition,the problems of differences of ingredients contents with different varieties breeds and the strong adverse effects were also discussed,aiming to provide a theoretical reference for the development and utilization of Perilla seed.

Key words: Perilla seed; perillaldehyde; livestock production; anti-inflammatory effect

中图分类号:

S816

姚玲珑, 宫宇, 陈清华, 陈凤鸣, 李安定. 紫苏籽活性成分的抗炎作用及其在畜牧生产中的应用研究进展[J]. 《中国畜牧兽医》, 2019, 46(1): 123-129.

YAO Linglong, GONG Yu, CHEN Qinghua, CHEN Fengming, LI Anding. Research Progress on Anti-inflammatory Effects of Active Components of Perilla Seeds and Their Application in Livestock Production[J]. , 2019, 46(1): 123-129.

参考文献

[1] 张占军.食叶红紫苏不同生育期主要营养成分分析研究[J].中国食物与营养,2014,20(4):69-72. ZHANG Z J.Analysis of the main nutrition ingredients of edible leaf red perilla in different growth stages[J].Food and Nutrition in China,2014,20(4):69-72.(in Chinese)
[2] YU S T,XIONG G X,CHENG H,et al.Influence of different extraction methods on Perilla frutescens leaf volatile components[J].Journal of Southern Agriculture,2014,45(1):108-111.
[3] ZHANG X B,JIANG W X,ZHANG L,et al.Research progress of Perilla frutescens[J].Food Research & Development,2015,36(7):140-143.
[4] CHEN M L,WU C H,HUNG L S,et al.Ethanol extract of Perilla frutescens suppresses allergen-specific Th2 responses and alleviates airway inflammation and hyper reactivity in ovalbumin-sensitized murine model of asthma[J].Evidence-Based Complementray and Alternative Medicine,2015,4(1):1-8.
[5] XU L,LI Y,FU Q,et al.Perillaldehyde attenuates cerebral ischemia-reperfusion injury-triggered overexpression of inflammatory cytokines via modulating Akt/JNK pathway in the rat brain cortex[J].Biochemical & Biophysical Research Communications,2014,454(1):65-70.
[6] 许万乐,李会珍,张志军,等.紫苏籽油理化性质测定及脂肪酸组分分析[J].中国粮油学报,2013,28(12):106-109. XU W L,LI H Z,ZHANG Z J,et al.Determination of physicochemical properties and analysis on fatty acid composition of different Perilla seed oil[J].Journal of the Chinese Cereals and Oils Association,2013,28(12):106-109.(in Chinese)
[7] 夏瑶瑶.紫苏籽油及原花青素的提取工艺研究[D].太原:中北大学,2017. XIA Y Y.Study on extraction technology of oil and procyanidins from Perilla seed[D].Taiyuan:North University of China,2017.(in Chinese)
[8] 赵晓玲.紫苏籽主要营养成分分析[J].中国食物与营养,2015,21(3):63-67. ZHAO X L.Analysis on main nutritional components in basil seed[J].Food and Nutrition in China,2015,21(3):63-67.(in Chinese)
[9] 李会珍,孙子文,李晓君,等.紫苏种子性状与主要营养成分相关性分析[J].中国粮油学报,2013,28(10):55-59. LI H Z,SUN Z W,LI X J,et al.Correlation analysisof seed traits and nutritional components in Perilla[J].Journal of the Chinese Cereals and Oils Association,2013,28(10):55-59.(in Chinese)
[10] 魏长玲,郭宝林,张琛武,等.中国紫苏资源调查和紫苏叶挥发油化学型研究[J].中国中药杂志,2016,41(10):1823-1834. WEI C L,GUO B L,ZHANG C W,et al.Perilla resources of China and essential oil chemotypes of Perilla leaves[J].China Journal of Chinese Materia Medica,2016,41(10):1823-1834.
[11] 时艺霖,顾宪红,毛薇,等.紫苏籽化学成分提取工艺条件及应用研究进展[J].家畜生态学报,2015,36(8):79-85. SHI Y L,GU X H,MAO W,et al.Research progress in the extraction technology conditions and application of chemical substances in Perilla frutescens seed[J].Acta Ecologiae Animalis Domastici,2015,36(8):79-85.(in Chinese)
[12] 贾青慧,沈奇,陈莉.紫苏籽蛋白质与氨基酸的含量测定及营养评价[J].食品研究与开发,2016,37(10):6-9. JIA Q H,SHEN Q,CHEN L.The content determination and nutritional evaluation of Perilla seed protein and amino acids[J].Food Research and Development,2016,37(10):6-9.(in Chinese)
[13] 邹茜.浅谈紫苏不同居群不同部位总黄酮含量的差异[J].当代医药论丛,2014,12(9):32-34. ZOU Q.Discussion on the difference of total flavonoids content in different parts of different populations of Perilla frutescens[J]. Contemporary Medical Symposium,2014,12(9):32-34.(in Chinese)
[14] ISHIKURA N.Anthocyanins and flavones in leaves and seeds of Perilla plant[J].Journal of the Agricultural Chemical Society of Japan,2006,45(8):1855-1860.
[15] NAKAJIMA A,YAMAMOTO Y,YOSHINAKA N,et al.A new flavanone and other flavonoids from green perilla leaf extract inhibit nitric oxide production in interleukin 1β-treated hepatocytes[J].Journal of the Agricultural Chemical Society of Japan,2015,79(1):138-146.
[16] LEE J H,PARK K H,LEE M H,et al.Identification,characterisation,and quantification of phenolic;Compounds in the antioxidant activity-containing fraction from the seeds of Korean Perilla (Perilla frutescens) cultivars[J].Food Chemistry,2013,136(2):843-852.
[17] YUE J S,YING W,HUI Z Z,et al.Characterization of constituents in Perilla frutescens decoction by liquid chromatography coupled with mass spectrometry[J].Chinese Journal of Pharmaceutical Analysis,2015,35(8):1417-1423.
[18] LILLIAN B,MONTSERRAT D A,ISABELC F R,et al.Use of HPLC-DAD-ESI/MS to profile phenolic compounds in edible wild greens from Portugal[J].Food Chemistry,2011,127(1):169-173.
[19] 杨慧,马培,林明宝,等.紫苏叶化学成分、抗炎作用及其作用机制研究进展[J].中国药理学与毒理学杂志,2017,31(3):279-286. YANG H,MA P,LIN M B,et al.Research progress in chemical constituents,anti-inflammatory effect and mechanism of Folium Perillae[J].Chinese Journal ofPharmacology and Toxicology,2017,31(3):279-286.(in Chinese)
[20] DIPASQUALE D,BASIRIC L,MORERA P,et al.Anti-inflammatory effects of conjugated linoleic acid isomers and essential fatty acids in bovine mammary epithelial cells[J].Animal,2018,2(10):2108-2114.
[21] WANG Y W,FIELD C J,SIM J S.Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation,serum immunoglobulin G concentration,and immune tissue development in chicks[J].Poultry Science,2000,79(12):1741-1748.
[22] 宋代军,谢君,杨游,等.紫苏籽提取物对肉鸡免疫机能的影响[J].中国兽医学报,2014,34(5):793-797. SONG D J,XIE J,YANG Y,et al.Effect of Perillafrutescens extract on immunity function of broilers[J].Chinese Journal of Veterinary Science,2014,34(5):793-797.(in Chinese)
[23] 周美玲.紫苏挥发油及其主要成分紫苏醛和柠檬烯对小鼠生长和免疫功能的影响[D].扬州:扬州大学,2014. ZHOU M L.Effects of Perilla frutescens volatile oil and its main components (perillaldehyde and limonene) on growth and immune function of mice[D].Yangzhou:Yangzhou University,2014.(in Chinese)
[24] UEMURA T,YASHIRO T,ODA R,et al.The intestinal anti-inflammatory activity of perillaldehyde[J].Journal of Agricultural and Food Chemistry,2018,66(13):3443-3448.
[25] KITAMURA N,NISHINO M,FUJⅡ A,et al. Perilla extract improves frequent urination in spontaneously hypertensive rats with enhancement of the urothelial presence and anti-inflammatory effects[J].International Journal of Urology,2017,25(3):298-304.
[26] FUYUNO Y,UCHI H,YASUMATSU M,et al.Perillaldehyde inhibits AHR signaling and activatesNRF2 antioxidant pathway in human keratinocytes[J].Oxidative Medicine & Cellular Longevity,2018,2018:1-9.
[27] KWON S J,LEE J H,MOON K D,et al.Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway[J].International Journal of Oncology,2014,45(5):1969-1976.
[28] IN C H,SO Y,NAM B,et al.Isoegomaketone alleviates the development of collagen antibody-induced arthritis in male Balb/c mice[J].Molecules,2017,22(7):1209.
[29] KURE A,NAKAGAWA K,KONDO M,et al.Metabolic fate of luteolin in rats:Its relationship to anti-inflammatory effect[J].Journal of Agricultural & Food Chemistry,2016,64(21):4246-4254.
[30] YAO Z H,YAO X L,ZHANG Y,et al.Luteolin could improve cognitive dysfunction by inhibiting neuroinflammation[J].Neurochemical Research,2018,43(9):1-15.
[31] 王书侠,张家明,姚孝明,等.木犀草素对活化的RAW264.7巨噬细胞分泌炎症因子的影响[J].医学研究生学报,2017,30(1):31-35. WANG S X,ZHANG J M,YAO X M,et al.Effects of luteolin on the secretion of inflammatory cytokines from activated RAW264.7 macrophages[J].Journal of Medical Postgraduates,2017,30(1):31-35.(in Chinese)
[32] 周亮,乔印玲,王莉,等.木犀草素通过抑制PTEN PI3K-AKT信号通路以及对免疫功能的调节作用介导对肺癌模型大鼠的干预研究[J].天然产物研究与开发,2017,29(12):2128-2133. ZHOU L,QIAO Y L,WANG L,et al.Effects of luteolin on the expression of PTEN PI3K-AKT signaling pathway and immune function in lung cancer model rats[J].Natural Product Research and Development,2017,29(12):2128-2133.(in Chinese)
[33] ROCHA J,EDUARDO-FIGUEIRA M,BARATEIRO A,et al.Anti-inflammatory effect of rosmarinic acid and an extract of rosmarinus officinalis in rat models of local and systemic inflammation[J].Basic & ClinicalPharmacology & Toxicology,2015,116(5):398-413.
[34] 姚杨,李蓉,苏杰,等.迷迭香酸对口腔溃疡大鼠炎症因子和免疫功能的影响[J].医学研究生学报,2018,31(1):29-32. YAO Y,LI R,SU J,et al.Effect of rosmarinic acid on inflammatory factors and immune function in oral ulcer rats[J].Journal Medical Postgraduate,2018,31(1):29-32.(in Chinese)
[35] 房祥杰,张彬,张德重,等.迷迭香酸通过MAPK/ERK信号通路对结肠癌细胞增殖及凋亡的影响[J].中国肿瘤,2018,27(4):306-310. FANG X J,ZHANG B,ZHANG D Z,et al.Effects of rosmarinic acid on proliferation and apoptosis of colon cancer cells via MAPK/ERK signaling pathway[J].China Cancer,2018,27(4):306-310.(in Chinese)
[36] 林淼,周美玲,霍永久,等.紫苏叶挥发油及紫苏醛、柠檬烯对小鼠生长性能、血液指标及粪便菌群的影响[J].中国饲料,2014,22:26-28. LIN M,ZHOU M L,HUO Y J.Effects of Perilla frutescens leaf volatile oil,perillaldehyde and limonene on growth performance,blood parameters and fecal bacteria flora in mice[J].China Feed,2014,22:26-28.(in Chinese)
[37] 时艺霖,顾宪红,黄勇,等.紫苏籽提取物对蛋鸡产蛋高峰后期生产性能、生殖激素及免疫功能的影响[J].动物营养学报,2015,27(5):1519-1526. SHI Y L,GU X H,HUANG Y,et al.Effects of Perilla frutescens seed extracts on performance,reproductive hormone and immune function of laying hens during the late laying peak period[J].Chinese Journal of Animal Nutrition,2015,27(5):1519-1526.(in Chinese)
[38] 石程仁,张初署,杜咏梅,等.不同植物抑制剂对黄曲霉菌生长和产毒的影响[J].食品安全质量检测学报,2017,8(8):2892-2897. SHI C R,ZHANG C F,DU Y M,et al.Effects of different plant inhibitors on the growth and toxin production of Aspergillus flavus[J].Journal of FoodSafety & Quality,2017,8(8):2892-2897.(in Chinese)
[39] 刘树兴,闫莉斐,陈蕊,等.紫苏油对益生菌的增殖作用研究[J].中国油脂,2018,43(1):61-63. LIU S X,YAN L F,CHEN R,et al.Effects of Perilla oil on proliferation of probiotics[J].China Oils and Fats,2018,43(1):61-63.(in Chinese)
[40] 赵必迁.紫苏籽提取物对杜长大猪屠宰性能和肉品质的影响[J].养猪,2018,2:70-72. ZHAO B Q.Effects of Perilla seed extract on slaughter performance and meat quality of DLY pigs[J].Pig Prodution,2018,2:70-72.(in Chinese)
[41] 潘存霞,谢君.紫苏籽提取物对肉鸡生长性能和鸡肉品质的影响[J].饲料工业,2011,32(21):12-14. PAN C X,XIE J.Effect of Perilla seed extract ongrowth performance and chicken quality of broilers[J].Feed Industry,2011,32(21):12-14.(in Chinese)
[42] DENG K,MA T,WANG Z,et al.Effects of Perilla frutescens seed supplemented to diet on fatty acid composition and lipogenic gene expression in muscle and liver of Hu lambs[J].Livestock Science,2018,Epub Ahead of Print.