催乳素对内蒙古绒山羊毛囊体外培养影响的研究

doi:10.16431/j.cnki.1671-7236.2022.06.007

摘要/Abstract

摘要： 【目的】研究催乳素（PRL）对内蒙古绒山羊初级毛囊和次级毛囊体外生长及形态变化的影响。【方法】机械法结合切割法分离内蒙古绒山羊的初级毛囊和次级毛囊，在初级毛囊培养液中分别添加0、5、10、50、100 ng/mL催乳素进行体外培养，每组24根，共培养5 d，每天在显微镜下观察其形态并拍照，统计其生长长度、生长速度和存活率，筛选出最适催乳素处理浓度。然后将初级毛囊与次级毛囊分别分为初级毛囊对照组（PF-K）、初级毛囊试验组（PF-PRL）、次级毛囊对照组（SF-K）、次级毛囊试验组（SF-PRL），每组24根，对照组用基础培养液培养，试验组在基础培养液中添加最适浓度的催乳素，培养5 d，每天观察毛囊的形态并拍照，同时测量各组毛囊的生长长度。【结果】 10 ng/mL催乳素组毛囊的平均日生长长度均极显著高于其他浓度组（P<0.01），最终生长长度和存活率均最高，因此，后续试验选择10 ng/mL催乳素处理毛囊。试验组和对照组初/次级毛囊的毛干与根鞘部位同时伸长，随着培养时间的增加均出现不同程度的弯曲。PF-PRL、SF-PRL组毛囊在2~5 d的总长度分别极显著高于PF-K、SF-K组（P<0.01）。PF-K组除第1天与第0天差异不显著外，1~5 d毛囊的总长度依次显著增加（P<0.05）；PF-PRL组0~5 d毛囊的总长度依次显著增加（P<0.05）。SF-K组毛囊第5天的总长度显著高于0~4 d （P<0.05）；SF-PRL组第4、5天毛囊的总长度均显著高于0~3 d （P<0.05），第3天毛囊的总长度显著高于0~2 d （P<0.05）。PF-PRL、SF-PRL组毛囊在2~5 d的平均日生长长度分别极显著高于PF-K、SF-K组（P<0.01）。【结论】 10 ng/mL催乳素是体外促进毛囊生长的最适浓度，10 ng/mL催乳素对体外培养的内蒙古绒山羊的初级毛囊和次级毛囊均有极显著的促生长作用。

关键词: 催乳素; 内蒙古绒山羊; 毛囊; 体外培养

Abstract: 【Objective】 The aim of this experiment was to study the effects of prolactin (PRL) on the growth and morphological changes of primary and secondary hair follicles in Inner Mongolia cashmere goats in vitro.【Method】 The primary and secondary hair follicles of Inner Mongolia cashmere goats were isolated by mechanical method combined with cutting method.The primary hair follicle culture medium was supplemented with 0, 5, 10, 50 and 100 ng/mL prolactin, respectively, with 24 roots in each group for 5 days in vitro culture.The morphology of the hair follicles was observed and photographed under a microscope every day.The growth length, growth rate and survival rate were calculated to screen out the optimal concentration of prolactin.Then, the primary and secondary hair follicles were divided into primary hair follicle control group (PF-K), primary hair follicle experimental group (PF-PRL), secondary hair follicle control group (SF-K) and secondary hair follicle experimental group (SF-PRL), with 24 hairs in each group.The control group was cultured with the basal medium, and the experimental group medium was supplemented with the optimal concentration of prolactin.The hair follicles were cultured for 5 days, the morphology of hair follicles were observed and took photos every day, and the growth length of each group was measured at the same time.【Result】 The average daily growth length of hair follicle in 10 ng/mL prolactin group was extremely significantly higher than that in other concentration groups, the final growth length and survival rate of hair follicles in 10 ng/mL prolactin group were the highest (P<0.01).Therefore, 10 ng/mL prolactin was selected for subsequent experiments.The stem and root sheath of primary/secondary hair follicles in experimental and control groups were elongated simultaneously, and curved to different degrees with the increase of culture time.The total length of hair follicles PF-PRL and SF-PRL groups at 2-5 days was significantly higher than that in PF-K and SF-K groups, respectively (P<0.01).The total length of hair follicles in PF-K group increased significantly from day 1 to 5, except that there was no significant difference between day 1 and 0 (P<0.05), the total length of hair follicles in PF-PRL group was increased significantly from 0 to 5 days (P<0.05).The total length of hair follicles in SF-K group on the 5th day was significantly higher than that in 0-4 days (P<0.05).The total length of hair follicles in SF-PRL group was significantly higher than 0-3 days on day 4 and 5 (P<0.05), and the total length of hair follicles on day 3 was significantly higher than 0-2 days (P<0.05).The average daily growth length of hair follicles in PF-PRL and SF-PRL groups was significantly higher than that in PF-K and SF-K groups (P<0.01).【Conclusion】 10 ng/mL prolactin was the optimal concentration to promote hair follicle growth in vitro.10 ng/mL prolactin could promote the growth of primary and secondary hair follicles of Inner Mongolia cashmere goats in vitro.

Key words: prolactin; Inner Mongolia cashmere goat; hair follicle; in vitro culture

中图分类号:

S857.4

包跃先, 赵飞飞, 宫文典, 卢泽宇, 穆卿, 刘俊阳, 特日格乐, 赵艳红. 催乳素对内蒙古绒山羊毛囊体外培养影响的研究[J]. 中国畜牧兽医, 2022, 49(6): 2072-2078.

BAO Yuexian, ZHAO Feifei, GONG Wendian, LU Zeyu, MU Qing, LIU Junyang, TE Rigele, ZHAO Yanhong. Effects of Prolactin on Hair Follicle Culture of Inner Mongolia Cashmere Goats in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2072-2078.

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