绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析

doi:10.16431/j.cnki.1671-7236.2021.06.004

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (6): 1918-1926.doi: 10.16431/j.cnki.1671-7236.2021.06.004

绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析

王立忠^1,2, 吴铁成^2,3, 马跃军², 李玉荣², 乌亚罕⁴, 何托雅⁵, 赵生国¹, 刘斌²

1. 甘肃农业大学动物科学技术学院, 兰州 730070;
2. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
3. 肉羊遗传资源评价与繁育技术内蒙古自治区工程实验室, 呼和浩特 010031;
4. 鄂托克前旗北极神绒牧业研究所, 鄂尔多斯 016200;
5. 鄂尔多斯市畜牧工作站, 鄂尔多斯 017000

收稿日期:2020-12-22 出版日期:2021-06-20 发布日期:2021-06-18
通讯作者: 赵生国,刘斌 E-mail:zhaosg@gsau.edu.cn;liubin0613@126.com
作者简介:王立忠(1995-),男,甘肃酒泉人,硕士生,研究方向:绒山羊遗传育种,E-mail:1838425986@qq.com;吴铁成(1988-),男,甘肃天水人,博士生,研究方向:绒山羊繁育与营养,E-mail:wutiec@qq.com
基金资助:
国家自然科学基金项目（31760653）；内蒙古自治区科技计划项目（2020GG0095）；内蒙古创新基金项目（2020CXJJM01）

Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity

WANG Lizhong^1,2, WU Tiecheng^2,3, MA Yuejun², LI Yurong², WU Yahan⁴, HE Tuoya⁵, ZHAO Shengguo¹, LIU Bin²

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Inner Mongolia Autonomous Region Academy of Agricultural and Husbandry Science, Hohhot 010031, China;
3. Engineering Laboratory for Genetic Resources Evaluation and Breeding Technology of Mutton Sheep in Inner Mongolia Autonomous Region, Hohhot 010031, China;
4. Etuokeqianqi Arctic God Research Institute of Cashmere and Livestock, Erdos 016200, China;
5. Erdos Animal Husbandry Workstation, Erdos 017000, China

Received:2020-12-22 Online:2021-06-20 Published:2021-06-18

摘要/Abstract

摘要： 为了探究chi-miR-107-3p和菱形家族蛋白2（rhomboid family member 2，RHBDF2）基因在不同品种（系）和光控增绒绒山羊兴盛前期（5~7月份）皮肤毛囊重建时的表达差异，本试验在7月份采集内蒙古阿拉善型绒山羊、敏盖绒山羊和光控增绒技术处理的阿尔巴斯型绒山羊体侧皮肤毛囊组织，采用组织切片技术对组织形态比较分析，实时荧光定量PCR法检测chi-miR-107-3p和RHBDF2基因的表达量。结果显示，在皮肤毛囊兴盛前期，阿拉善型绒山羊次级毛囊正处于重建阶段，敏盖绒山羊和阿尔巴斯型绒山羊（光控增绒）次级毛囊重建已基本完成；在阿拉善型绒山羊皮肤组织中chi-miR-107-3p表达量极显著高于敏盖绒山羊和阿尔巴斯型绒山羊（光控增绒）（P<0.01），而RHBDF2基因表达量极显著低于其他两品种（系）（P<0.01），且RHBDF2基因表达量在敏盖绒山羊皮肤组织中显著低于阿尔巴斯型绒山羊（P<0.05）。不同品种（系）和光控增绒绒山羊皮肤毛囊兴盛前期组织显微结构与chi-miR-107-3p、RHBDF2基因在皮肤组织中的表达差异分析结果相一致，次级毛囊重建初期chi-miR-107-3p表达量较高，而RHBDF2基因表达量极低，随着次级毛囊重建完成，chi-miR-107-3p表达量明显降低，RHBDF2基因表达量逐渐增高，且在不同品种（系）内蒙古绒山羊绒毛生长发育过程中的表达机制基本相同。因此，chi-miR-107-3p和RHBDF2基因是绒山羊皮肤毛囊生长发育的重要调控因子。

关键词: 内蒙古绒山羊; 光控增绒; 组织形态; chi-miR-107-3p; RHBDF2基因

Abstract: The aim of this study was to explore the effect of different expressions of chi-miR-107-3p and rhomboid family member 2(RHBDF2) genes on the villus growth of cashmere goats in different breeds (strains) and light controlled cashmere increasing in the skin hair follicle anagen (May to July).In this experiment,the hair follicle tissues of Inner Mongolia Alashan cashmere goats,Mingai cashmere goats and Albas cashmere goats which were used the increasing cashmere yield technology by shortened photoperiod were randomly collected in July,and HE staining was used to observe the morphology differences.Real-time quantitative PCR was used to detect the ralative expression of chi-miR-107-3p and RHBDF2 genes.The results showed that the secondary hair follicles of Alashan cashmere goats were in the stage of growth and development,and the reconstruction of secondary hair follicles of Mingai cashmere goats and Albas cashmere goats (light controlled cashmere increasing) had been basically completed.The expression of chi-miR-107-3p in the skin tissue of Alashan cashmere goats was extremely significantly higher than that of Mingai cashmere goats and Albas cashmere goats (P<0.01),the expression level of RHBDF2 gene was extremely significantly lower than that of the other two breeds (P<0.01),but the expression level of RHBDF2 gene in skin tissue of Mingai cashmere goats was significantly lower than that of Albas cashmere goats (P<0.05).In conclusion,the microstructure of hair follicle in different breeds (strains) light controled cashmere goats at anagen prosperity was consistent with the results of the differential analysis of chi-miR-107-3p and RHBDF2 genes in skin tissues.The expression level of chi-miR-107-3p was high,while the expression level of RHBDF2 gene was extremely low.With the completion of secondary hair follicle reconstruction,the expression of chi-miR-107-3p decreased significantly,the expression of RHBDF2 gene increased gradually,and the expression mechanism of Inner Mongolia cashmere in different breeds (strains) was basically the same.chi-miR-107-3p and RHBDF2 genes were important regulators for the growth and development of skin hair follicles in cashmere goats.

Key words: Inner Mongolia cashmere goats; light controlled cashmere increasing; histomorphology; chi-miR-107-3p; RHBDF2 gene

中图分类号:

Q786

王立忠, 吴铁成, 马跃军, 李玉荣, 乌亚罕, 何托雅, 赵生国, 刘斌. 绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析[J]. 中国畜牧兽医, 2021, 48(6): 1918-1926.

WANG Lizhong, WU Tiecheng, MA Yuejun, LI Yurong, WU Yahan, HE Tuoya, ZHAO Shengguo, LIU Bin. Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1918-1926.

参考文献

[1] 李长青,尹俊,张燕军,等.内蒙古绒山羊与辽宁绒山羊皮肤毛囊周期性变化的比较研究[J].畜牧兽医学报,2005,36(7):674-679. LI C Q,YIN J,ZHANG Y J,et al.Comparative study on skin and hair follicles cycling between Inner Mongolia and Liaoning cashmere goats[J].Chinese Journal of Animal and Veterinary Sciences,2005,36(7):674-679.(in Chinese)
[2] YANG M,SONG S,DONG K,et al.Skin transcriptome reveals the intrinsic molecular mechanisms underlying hair follicle cycling in cashmere goats under natural and shortened photoperiod conditions[J].Scientific Reports,2017,7(1):132-142.
[3] 赵艳丽,张世伟,姜怀志.辽宁绒山羊两品系皮肤毛囊结构及其活性变化规律的比较[J].吉林农业大学学报,2009,31(6):746-751. ZHAO Y L,ZHANG S W,JIANG H Z.Comparative study on hair follicles stucture and cycling between two strains of Liaoning cashmere goats[J].Journal of Jilin Agricultural University,2009,31(6):746-751.(in Chinese)
[4] 勿都巴拉,乌亚罕,李玉荣,等.短光照对绒山羊绒毛生长相关激素的影响[J].华中农业大学学报,2018,37(1):75-81. WUDUBALA,WU Y H,LI Y R,et al.Effect of short photoperiod on hair growth related hormones in cashmere goat[J].Journal of Huazhong Agricultural University,2018,37(1):75-81.(in Chinese)
[5] 刘丹丹,赫晓燕,郝欢庆.microRNAs在动物皮肤和毛发发育中的调控作用[J].中国生物化学与分子生物学报,2010,26(9):802-808. LIU D D,HE X Y,HAO H Q.The regulatory role of microRNAs in the development of animal skin and hair[J].Chinese Journal of Biochemistry and Molecular Biology,2010,26(9):802-808.(in Chinese)
[6] LEE R C,FEINBAUM R L,AMBROS V.The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J].Comparative Study,1993,75(5):843-854.
[7] 赵志东,徐华荣,屈雷,等.羊绒生长期、休止期陕北白绒山羊皮肤组织中KAP9.2和Hoxc13基因的表达[J].西北农林科技大学学报(自然科学版),2012,40(9):24-28. ZHAO Z D,XU H R,QU L,et al.Expression analysis of KAP9.2 and Hoxc13 gene in skin tissues during anagen and telogen of Shaanbei White cashmere goat[J].Journal of Northwest A&F University (Natural Science Edition),2012,40(9):24-28.(in Chinese)
[8] 张桂山,徐晶,姜怀志.microRNA对皮肤毛囊发育调控的研究进展[J].家畜生态学报,2013,34(6):1-4. ZHANG G S,XU J,JIANG H Z.Research progress on microRNA regulation on skin and hair follicle development[J].Journal of Domestic Animal Ecology,2013,34(6):1-4.(in Chinese)
[9] YUAN S K,LI F F,MENG Q Y,et al.Post-transcriptional regulation of keratinocyte progenitor cell expansion,differentiation and hair follicle regression by miR-22[J].PLoS Genetics,2015,11(5):e1005253.
[10] 马涛.miRNA-203在绒山羊毛囊发育周期中的差异表达及其靶基因功能鉴定[D].长春:吉林大学,2019. MA T.Differential expression of miRNA-203 in hair follicle development cycle of cashmere goats and identification of target gene function[D].Changchun:Jilin University,2019.(in Chinese)
[11] 何玉龙,陶宇,李勇,等.不同发育阶段小尾寒羊皮肤毛囊特异性miRNA的鉴定[J].农业生物技术学报,2019,27(1):89-96. HE Y L,TAO Y,LI Y,et al.Identification of specific miRNA in skin follicles of Small-tail Han sheep (Ovis aries) at different developmental stages[J].Journal of Agricultural Biotechnology,2019,27(1):89-96.(in Chinese)
[12] HOSUR V,JOHNSON K R,BURZENSKI L M,et al.RHBDF2 mutations increase its protein stability and drive EGFR hyperactivation through enhanced secretion of amphiregulin[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(21):7510.
[13] HOSUR V,BURZENSKI L M,STEARS T M,et al.Early induction of NRF2 antioxidant pathway by RHBDF2 mediates rapid cutaneous wound healing[J].Experimental and Molecular Pathology,2017,102(2):337-346.
[14] HOSUR V,LYONS B L,BURZENSKI L M,et al.Tissue-specific role of RHBDF2 in cutaneous wound healing and hyperproliferative skin disease[J].BMC Research Notes,2017,10(1):573.
[15] 吴丽媛,刘斌,辛雷勇,等.内蒙古阿尔巴斯型绒山羊光控增绒效果分析[J].中国畜牧兽医,2018,45(7):1972-1977. WU L Y,LIU B,XIN L Y,et al.Effect of light on cashmere growth of Inner Mongolia Arbas cashmere goats[J].China Animal Husbandry & Veterinary Medicine,2018,45(7):1972-1977.(in Chinese)
[16] 李桂芳,赵宗胜,李大全,等.绵羊皮肤结构观察与羊毛生长调控[J].草食家畜,2002,4:56-59. LI G F,ZHAO Z S,LI D Q,et al.The observation of derma structure and control of wool growth[J].Grass-Feeding Livestock,2002,4:56-59.(in Chinese)
[17] 刘斌,郭俊,勿都巴拉,等.光周期对于绒山羊毛囊干细胞激活的影响[J].中国畜牧兽医,2017,44(4):994-1000. LIU B,GUO J,WUDUBALA,et al.Effects of photoperiod on stem cells activation in cashmere goat hair follicles[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):994-1000.(in Chinese)
[18] ZHANG C Z,SUN H Z,CHUN H Z,et al.Effects of photoperiod on circadian clock genes in skin contribute to the regulation of hair follicle cycling of Inner Mongolia White cashmere goats[J].Animal Science Journal,2020,91(1):e13320.
[19] 吴月红,李勇,杨易,等.microRNAs在动物皮肤及毛囊发育中的调控作用研究进展[J].中国畜牧兽医,2016,43(9):2373-2378. WU Y H,LI Y,YANG Y,et al.Research progress on regulation of micorRNAs in animal skin and hair follicle[J].China Animal Husbandry & Veterinary Medicine,2016,43(9):2373-2378.(in Chinese)
[20] 张桂山,徐晶,孙丽敏,等.不同品种羊miR-1298-5p及其潜在靶基因TGF-βR1表达的比较研究[J].中国农学通报,2017,33(27):49-54. ZHANG G S,XU J,SUN L M,et al.Comparative study on expression of miR-1298-5p and its potential target gene TGF-βR1 in Fine-wool sheep and cashmere goats[J].Chinese Agricultural Science Bulletin,2017,33(27):49-54.(in Chinese)
[21] 曲海娥.miR-125b在细毛羊与绒山羊皮肤组织中差异表达及其靶基因的筛选与鉴定[D].长春:吉林大学,2014. QU H E.Differential expression of miR-125b between the skin of cashmere goats and Fine-wool sheep,screen and identify its targets[D].Changchun:Jilin University,2014.(in Chinese)
[22] PAOLA A,CATHERINE M W,MATTTEHW A B,et al.p63 is a key regulator of iRHOM2 signalling in the keratinocyte stress response[J].Nature Communications,2018,9(1):615-634.
[23] THIVIYANI M,ANISSA C,BENJAMIN F,et al.Rhomboid family member 2 regulates cytoskeletal stress-associated keratin 16[J].Nature Communications,2017,8(1):767-775.

绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析

Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity

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