塔里木马鹿干旱环境适应相关基因的筛选、克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2021.06.003

摘要/Abstract

摘要： 为了探讨塔里木马鹿（Cervus elaphus yarkandensis）干旱环境适应相关基因的结构特征和相关功能，从前期的塔里木马鹿全基因组重测序结果中，筛选获得塔里木马鹿过氧化物氧化还原酶3（thioredoxin-dependent peroxide reductase，PRDX3）基因的序列，对该基因在塔里木马鹿不同组织中的表达情况进行分析，同时对塔里木马鹿PRDX3基因编码区（CDS）序列进行克隆测序，运用相关软件进行同源性比对、构建系统进化树及生物信息学分析。结果显示，塔里木马鹿PRDX3基因在肾脏组织中的基因表达水平极显著高于肺脏和肝脏组织（P<0.01）；塔里木马鹿PRDX3基因CDS序列长660 bp。同源性比对和系统进化树分析结果表明，塔里木马鹿与白尾鹿（GenBank登录号：XM_020875097.1）同源性最高，且遗传距离最近；与褐家鼠（GenBank登录号：NM_022540.1）同源性最低，且遗传距离最远。塔里木马鹿PRDX3蛋白分子质量为24.42 ku，由220个氨基酸组成，不稳定系数为25.36，理论等电点（pI）为5.82，脂溶系数为85.50，总平均亲水性为-0.05，存在O-糖基化位点，具有丰富的磷酸化位点，但是不存在N-糖基化位点、跨膜区及信号肽，最可能位于线粒体中，二级结构和三级结构主要由α-螺旋和无规则卷曲组成，包含PRX_Typ2cys超家族保守结构域，与多种蛋白存在相对较强的相互作用。本研究为后续的塔里木马鹿功能基因的研究奠定基础。

关键词: 塔里木马鹿; 干旱环境适应; PRDX3基因; 生物信息学分析

Abstract: In order to explore the structural and functions of the genes related to the adaptation of Tarim red deer(Cervus elaphus yarkandensis) to the arid environment.The sequence of thioredoxin dependent peroxidase 3 (PRDX3) gene of Tarim red deer was screened from the results of whole genome resequencing,and analyzed the expression of this gene in different tissues of Tarim red deer.The CDS sequence of PRDX3 gene of Tarim red deer was cloned and sequenced.Homology comparison,phylogenetic tree construction and bioinformatics analysis were carried out by using related software.The results showed that the expression level of PRDX3 gene in kidney was extremely significantly higher than that in lung and liver (P<0.01).The CDS region of PRDX3 gene of Tarim red deer was 660 bp in length and encoded 220 amino acids.The bioinformatics analysis using related software showed that Tarim red deer and Odocoileus virginianus (GenBank accession No.:XM_020875097.1) had the highest homology and the closest genetic distance,and with Rattus norvegicus (GenBank accession No.:NM_022540.1) had the lowest homology and the longest genetic distance.The protein molecular weight of PRDX3 in Tarim red deer was 24.42 ku and composed of 220 amino acids.It was predicted that the instability index,theoretical isoelectric point,lipid solubility index and hydrophilic average coefficient of PRDX3 protein was 25.36,5.82,85.50,and -0.05,respectively.There was O-glycosylation site and phosphorylation sites in PRDX3,but had no transmembrane region,N-glycosylation site and signal peptide,most likely located in the mitochondria.The secondary and tertiary structures of PRDX3 protein were mainly comprised of α-helix and random coil.PRDX3 protein contained the conserved domain of PRX_Typ2cys superfamily,which had relatively strong interaction with a variety of proteins.The results of this study laid a foundation for the following functional gene research of Tarim red deer.

Key words: Tarim red deer; drought adaptation; PRDX3 gene; bioinformatics analysis

中图分类号:

塔依尔江·麦麦提, 苏比奴尔·艾力, 布威海丽且姆·阿巴拜科日, 马合木提·哈力克. 塔里木马鹿干旱环境适应相关基因的筛选、克隆及生物信息学分析[J]. 中国畜牧兽医, 2021, 48(6): 1906-1917.

MAMAT Tayerjan, ELI Subinur, ABABAIKERI Buweihailiqiemu, HALIK Mahmut. Screening,Cloning and Bioinformatics Analysis of Drought Adaptation Related Genes in Tarim Red Deer[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1906-1917.

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