麝鼠香腺Elovl1基因生物信息学分析及表达研究

doi:10.16431/j.cnki.1671-7236.2022.04.001

摘要/Abstract

摘要： 【目的】测定麝鼠超长链脂肪酸延伸酶1(elongase of verylong chain fatty acid 1,Elovl1)基因序列并进行生物信息学分析,探讨其在麝鼠香腺发育和泌香过程中的作用。【方法】使用TRIzol法提取成年雄性麝鼠香腺总RNA,采用转录组测序技术获得麝鼠香腺Elovl1基因序列并测序;在GenBank数据库中下载啮齿目不同物种的Elovl1基因及其多转录本CDS序列并构建系统进化树;通过在线工具对Elovl1基因编码蛋白的理化性质及结构进行预测与分析;利用实时荧光定量PCR技术分析成年雄性麝鼠香腺发育周期内香腺组织中Elovl1基因的表达水平。【结果】 Elovl1基因在麝鼠香腺中表达2个转录本,能编码2个不同的蛋白,分别由313和279个氨基酸组成。Elovl1基因2个转录本在2~9月末的麝鼠香腺中均有表达,且表达量整体呈先上升后下降的趋势。Elovl1基因编码的2个蛋白均为稳定的亲脂性、非分泌蛋白,具有7个跨膜螺旋区,亚细胞定位均主要位于内质网。在啮齿目动物中,Elovl1基因进化与物种进化基本一致,仓鼠科与鼠科亲缘关系较近,麝鼠与水鼠平亲缘关系最近;同一物种Elovl1基因的不同转录本间相对保守,均按物种聚在一起且置信值普遍较高,旱獭例外。【结论】 Elovl1基因表达规律与麝鼠香腺的发育、萎缩及泌香周期相吻合,提示Elovl1与麝鼠香分泌相关,并参与了香腺细胞发育。

关键词: 麝鼠; 香腺; Elovl1基因; 表达; 生物信息学分析

Abstract: 【Objective】 This study was aimed to determine the nucleotide sequence of elongase of verylong chain fatty acid 1 (Elovl1) gene in muskrat and analyze its bioinformatics,and explore its role in the development and secretion of muskrat scent gland.【Method】 The total RNA was extracted from adult male muskrat’s fragrant gland by TRIzol method,the sequence of Elovl1 gene in muskrat scent gland was obtained by transcriptome sequencing.The CDS sequence of Elovl1 gene and its multiple transcripts of different species of rodents were downloaded in GenBank database,and phylogenetic tree was constructed.The physicochemical property and structure of Elovl1 gene encoded protein were predicted and analyzed using online tools.The transcription level of Elovl1 gene was analyzed in scent gland tissue during the developmental period of adult male muskrat by Real-time quantitative PCR.【Result】 There were two transcripts of Elovl1 gene in muskrat scent gland,which could encode two different proteins,with lengths of 313 and 279 amino acids,respectively.Both transcripts of Elovl1 gene were expressed in muskrat scent gland from the end of February to the end of September,and the overall expression level showed a trend of up-regulation first and then down-regulation.The two proteins encoded by Elovl1 gene were bothstable lipophilic and non-secreted proteins,with 7 transmembrane helix regions,and their subcellular locations were mainly in the endoplasmic reticulum.In rodents,the genetic evolution of Elovl1 gene was basically the same as that of species.Circetidae and Muridae were closely related,Ondatra zibethicus and Arvicola amphibius were closely related.The different transcripts of Elovl1 gene of the same species were relatively conserved,both grouped by species and generally had higher confidence values,with the exception of Marmota.【Conclusion】 The expression pattern of Elovl1 gene was consistent with the development,atrophy and secretion period of muskrat scent gland,suggesting that Elovl 1 was related to muskrat scent secretion,and should be involved in the development of scent gland cells.

Key words: muskrat; scent gland; Elovl1 gene; expression; bioinformatics analysis

中图分类号:

雷行, 李天峰, 李嘉昊, 满欣, 白素英. 麝鼠香腺Elovl1基因生物信息学分析及表达研究[J]. 中国畜牧兽医, 2022, 49(4): 1201-1212.

LEI Xing, LI Tianfeng, LI Jiahao, MAN Xin, BAI Suying. Bioinformatics Analysis and Expression of Elovl1 Gene in Muskrat Scent Gland[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1201-1212.

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