绵羊毛囊及其角蛋白研究进展

doi:10.16431/j.cnki.1671-7236.2021.01.027

摘要/Abstract

摘要： 毛囊是毛纤维的生长基础，决定毛纤维的性状，角蛋白是毛囊组成的重要成分之一。近年来，对毛囊生长发育和角蛋白基因的挖掘等取得了一定的进展。文章回顾了绵羊角蛋白基因的发现，已经与人类角蛋白基因相当，但在数量和分类上存在差异；笔者介绍了绵羊毛囊的形态学结构，简述了毛囊角蛋白基因的表达定位和影响毛纤维性状的角蛋白，并分析了毛囊中的蛋白质组学技术的应用对毛囊生长发育和毛纤维性状的重要作用，以期为绵羊毛囊生长发育和羊毛性状的分子选育提供新的研究思路。

关键词: 毛囊; 角蛋白; 定位; 纤维性状; 蛋白质组学技术

Abstract: Hair follicles are the basis for the growth of hair fibers,and determine the properties of wool fibers.Keratin is one of the important components of hair follicles.In recent years,some progresses have been made in hair follicle growth and development and the mining of keratin genes.The article reviewed the discovery of keratin genes,which were already equivalent to human keratin genes,but there were differences in number and classification.This paper introduced the morphological structure of hair follicles,briefly described the gene expression and localization of hair follicles keratin gene and the keratin influencing the character of hair fibers.The important effect of proteomics technology in hair follicle on the growth and development of hair follicle and fiber character was analyzed,in order to provide a new research idea for the growth and development of sheep hair follicles and the molecular breeding of wool traits.

Key words: hair follicle; keratin; localization; fiber traits; proteomics technology

中图分类号:

S826

和东迁, 陶金忠, 陈丽尧, 王俊奎. 绵羊毛囊及其角蛋白研究进展[J]. 中国畜牧兽医, 2021, 48(1): 248-256.

HE Dongqian, TAO Jinzhong, CHEN Liyao, WANG Junkui. Research Progress of Sheep Hair Follicle and Keratin[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 248-256.

参考文献

[1] ROGERS M A,LANGBEIN L,WINTER H,et al.Hair keratin associated proteins:Characterization of a second high sulfur KAP gene domain on human chromosome 211[J].Journal of Investigative Dermatology,2004,122(1):147-158.
[2] 燕荣帅,裴卓,马小艮,等.常见毛囊细胞角蛋白在毛囊周期中的表达研究[J].中华细胞与干细胞杂志(电子版),2019,9(1):7-12. YAN R S,PEI Z,MA X G,et al.Expression patterns of common hair follicle keratins in hair cycle[J].Chinese Journal of Cells and Stem Cells(Electrotic Version),2019,9(1):7-12.(in Chinese)
[3] LANGBEIN L,ROGERS M A,WINTER H,et al.The catalog of human hair keratins:Ⅰ.Expression of the nine type Ⅰ members in the hair follicle[J].The Journal of Biological Chemistry,1999,274(28):19874-19884.
[4] 王亚娟,高峰,刘迎春,等.不同浓度神经肽P物质刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响[J].中国畜牧兽医,2015,42(9):2298-2302. WANG Y J,GAO F,LIU Y C,et al.Effect of different concentrations of neuropeptide P on expression of BMP2 and BMP4 genes in skin stem cells of Mongolia Cashmere goat[J].China Animal Husbandry & Veterinary Medicine,2015,42(9):2298-2302.(in Chinese)
[5] PLOWMAN J E,HARLAND D P,GANESHAN S,et al.The proteomics of wool fibre morphogenesis[J].Journal of Structural Biology,2015,191(3):341-351.
[6] LIU G,LIU R,TANG X,et al.Expression profiling reveals genes involved in the regulation of wool follicle bulb regression and regeneration in sheep[J].International Journal of Molecular Sciences,2015,16(5):9152-9166.
[7] LANGBEIN L,ROGERS M A,PRAETZEL-WUNDER S,et al.K25(K25irs1),K26(K25irs2),K27(K25irs3),and K28(K25irs4) represent the type Ⅰ inner root sheath keratins of the human hair follicle[J].Journal of Investigative Dermatology,2006,126(11):2377-2386.
[8] DONALD F O,JOY L W,STEPHEN L R,et al.Cortical cell types and their distribution in wool fibres[J].Australian Journal of Biological Sciences,1984,37(4):237-256.
[9] HARLAND D P,VERNON J A,WOODS J L,et al.Intrinsic curvature in wool fibres is determined by the relative length of orthocortical and paracortical cells[J].The Journal of Experimental Biology,2018,221(Pt 6):jeb172312.
[10] 阿布来提·苏来曼,田月珍,许琴,等.苏博美利奴羊胎儿皮肤毛囊结构及形态发育[J].中国农业科学,2017,50(16):3226-3235. ABLAT S,TIAN Y Z,XU Q,et al.Study on fetal skin hair follicle structure and morphological development of Subo Merino sheep[J].Scientia Agricultura Sinica,2017,50(16):3226-3235.(in Chinese)
[11] SCHWEIZER J,BOWDEN P E,COULOMBE P A,et al.New consensus nomenclature for mammalian keratins[J].Journal of Cell Biology,2006,174(2):169-174.
[12] MOLL R,DIVO M,LANGBEIN L,et al.The human keratins:Biology and pathology[J].Histochemistry and Cell Biology,2008,129(6):705-733.
[13] YU Z D,PLOWMAN J E,MACLEAN P,et al.Ovine keratome:Identification,localisation and genomic organisation of keratin and keratin-associated proteins[J].Animal Genetics,2018,49(5):361-370.
[14] YU Z D,GORDON S W,WILDERMOTH J E,et al.Identification of novel wool keratin intermediate filament genes in sheep skin[J].Proceedings of the New Zealand Society of Animal Production,2010,70:222-228.
[15] CLERENS S,CORNELLISON C D,DEB-CHOUDHURY S,et al.Developing the wool proteome[J].Journal of Proteomics,2010,73(9):1722-1731.
[16] KANG X L,LIU Y F,ZHANG J B,et al.Characteristics and expression profile of KRT71 screened by suppression subtractive hybridization cDNA library in curly fleece Chinese Tan sheep[J].DNA & Cell Biology,2017,36(7):552-564.
[17] DOWLING L M,CREWTHER W G,INGLIS A S,et al.The primary structure of component 8c-1,a subunit protein of intermediate filaments in wool keratin.Relationships with proteins from other intermediate filaments[J].The Biochemical Journal,1986,236(3):695-703.
[18] POWELL B C,CROCKER L A,ROGERS G E,et al.Hair follicle differentiation:Expression,structure and evolutionary conservation of the hair type Ⅱ keratin intermediate filament gene family[J].Development,1992,114(2):417-433.
[19] WILSONK B W,EDWARDS J,SLEIGH M J,et al.Complete sequence of a type Ⅰ microfibrillar wool keratin gene[J].Gene,1988,73:21-31.
[20] SPARROW L G,ROBINSON C P,MCMAHON D,et al.The amino acid sequence of component 7c,a type Ⅱ intermediate-filament protein from wool[J].The Biochemical Journal,1989,261(3):1015-1022.
[21] SPARROW L G,ROBINSON C P,CAINE J,et al.Type Ⅱ Intermediate-filament proteins from wool.The amino acid sequence of component 5 and comparison with component 7c[J].The Biochemical Journal,1992,282(Pt 1):291-297.
[22] POWELL B C,CROCKER L A,ROGERS G E,et al.Complete sequence of a hair-like intermediate filament type Ⅱ keratin gene[J].DNA Sequence,1993,3(6):401-405.
[23] YU Z D,WILDERMOTH J E,WALLACE O A,et al.Annotation of sheep keratin intermediate filament genes and their patterns of expression[J].Experi-mental Dermatology,2011,20(7):582-588.
[24] HYND P I,EDWARDS N M,HEBART M,et al.Wool fibre crimp is determined by mitotic asymmetry and position of final keratinisation and not ortho-and para-cortical cell segmentation[J].Animal,2009,3(6):838-843.
[25] YU Z D,GORDON S W,NIXON A J,et al.Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles[J].Differentiation,2009,77(3):307-316.
[26] 刘春洁,付雪峰,田可川,等.新吉细毛羊角蛋白的EST筛选及表达研究[J].中国畜牧兽医,2015,42(11):2873-2879. LIU C J,FU X F,TIAN K C,et al.Screening and expression of keratin EST in Xinji fine-wool sheep[J].China Animal Husbandry & Veterinary Medicine,2015,42(11):2873-2879.(in Chinese)
[27] CHAI W,ZHOU H,FORREST R H J,et al.Polymorphism of KRT83 and its association with selected wool traits in Merino-cross lambs[J].Small Ruminant Research,2017,155:6-11.
[28] 李文浩.绵羊角蛋白基因鉴定及毛用性状关联性研究[D].兰州:甘肃农业大学,2019. LI W H.Identification of ovine keratin genes and correlation on wool traits[D].Lanzhou:Gansu Agricultural University,2019.(in Chinese)
[29] SULAYMAN A,TURSUN M,SULAIMAN Y,et al.Association analysis of polymorphisms in six KRT genes with wool traits in sheep[J].Asian Australa-sian Journal of Animal Sciences,2017,31(6):775-783.
[30] 晏华春.和田羊KAPs、KRT候选基因的遗传变异与羊毛细度和长度的相关性分析[D].乌鲁木齐:新疆农业大学,2015. YAN H C.Ananlysis of KAPs,KRT candidate genetic variation Hotan sheep correlated with the fineness and length of wool characteristics[D].Urumqi:Xinjiang Agricultural University,2015.(in Chinese)
[31] 牛姝,李淑敏,何清,等.毛发弯曲度相关蛋白KRT71和β-catenin在绵羊毛囊的定位表达[J].中国兽医科学,2017,47(5):660-663. NIU S,LI S M,HE Q,et al.Expression and localization of KRT71 and β-catenin in sheep hair follicle[J].Chinese Veterinary Science,2017,47(5):660-663.(in Chinese)
[32] 余娟娟,刘积凤,赵金山,等.敖汉细毛羊Ⅰ型内根鞘角蛋白基因表达模式的研究分析[J].安徽农业科学,2012,40(18):9784-9786. YU J J,LIU J F,ZHAO J S,et al.Genechip analysis of type Ⅰ IRS keratin expression pattern in Aohan wool sheep[J].Journal of Anhui Agricultural Sciences,2012,40(18):9784-9786.(in Chinese)
[33] 田月珍.中国美利奴羊(新疆型)羊毛细度相关候选基因的筛选及其关联性分析[D].乌鲁木齐:新疆农业大学,2012. TIAN Y Z.Study on selection and relation of candidate gene and associated with wool fineness in Merino sheep (Xinjiang type)[D].Urumqi:Xinjiang Agricultural University,2012.(in Chinese)
[34] NIKOLAY V Z,MIKHAIL Y S,ANDREY V M,et al.Autosomal recessive hypotrichosis with woolly hair caused by a mutation in the keratin 25 gene expressed in hair follicles[J].Journal of Investiga-tive Dermatology,2016,136(6):1097-1105.
[35] WESTGATE G E,GINGER R S,GREEN M R.The biology and genetics of curly hair[J].Experimental Dermatology,2017,26(6):483-490.
[36] 杨佐青.滩羊皮肤组织差异蛋白质组学研究[D].银川:宁夏大学,2017. YANG Z Q.Study of differential proteomics of Tan sheep skin tissue[D].Yinchuan:Ningxia University,2017.(in Chinese)
[37] 高丽霞,张燕军,张文广,等.内蒙古白绒山羊毛囊发育周期蛋白质表达谱分析[J].农业生物技术学报,2014,22(6):727-735. GAO L X,ZHANG Y J,ZHANG W G,et al.Analysis of protein expression profile of hair follicle cycle of Inner Mongolia Cashmere goat(Capra cashmere)[J].Journal of Agricultural Biotechnology,2014,22(6):727-735.(in Chinese)
[38] 杨洁.不同纤维直径的细毛羊皮肤组织中毛囊差异蛋白质双向电泳与质谱分析[D].乌鲁木齐:新疆农业大学,2014. YANG J.Studies on different of proteins in hair follicles of the skin tissue of fine-wool sheep with different fibre diameter using two-dimensional electrophoresis and mass spectrometry[D].Urumqi:Xinjiang Agricultural University,2014.(in Chinese)
[39] 付雪峰,杨涵羽璐,石刚,等.不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究[J].中国畜牧兽医,2016,43(4):879-891. FU X F,YANG H Y L,SHI G,et al.Proteome array on differentially expressed of skin tissue in fine-wool sheep with different fiber diameter[J].China Animal Husbandry & Veterinary Medicine,2016,43(4):879-891.(in Chinese)
[40] PLOWMAN J E,WOODS J L,VAN S B,et al.Preparation of wool follicles forproteomic studies[J].Analytical Biochemistry,2017,539:8-10.