杜洛克猪MAP3K5基因多态位点与生长、饲料利用性状的关联分析

doi:10.16431/j.cnki.1671-7236.2016.11.026

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 2982-2988.doi: 10.16431/j.cnki.1671-7236.2016.11.026

杜洛克猪MAP3K5基因多态位点与生长、饲料利用性状的关联分析

蒲蕾^1,2, 岳静伟¹, 张金山¹, 刘欣¹, 王立刚¹, 颜华¹, 侯欣华¹, 高红梅¹, 张恬¹, 张跃博¹, 武群清^1,3, 张龙超¹, 王立贤¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 天津农学院动物科学与动物医学学院, 天津 300384;
3. 湖南农业大学动物科学技术学院, 长沙 410128

收稿日期:2016-05-16 出版日期:2016-11-20 发布日期:2016-11-18
通讯作者: 张龙超, 王立贤 E-mail:zhlchias@163.com;iaswlx@263.net
作者简介:蒲蕾(1987-),女,陕西西安人,博士,研究方向:猪遗传育种,E-mail:pulei87@126.com
基金资助:
中国农业科学院科技创新工程（ASTIP-IAS02）；国家“十二五”科技支撑计划项目（2015BAD03B02-2）；国家现代农业产业技术体系（CARS-36）

Association Analysis on MAP3K5 Gene Polymorphism Site with Growth and Feed Efficiency Related Traits in Duroc Pigs

PU Lei^1,2, YUE Jing-wei¹, ZHANG Jin-shan¹, LIU Xin¹, WANG Li-gang¹, YAN Hua¹, HOU Xin-hua¹, GAO Hong-mei¹, ZHANG Tian¹, ZHANG Yue-bo¹, WU Qun-qing^1,3, ZHANG Long-chao¹, WANG Li-xian¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
3. College of Animal Science Technology, Hunan Agricultural University, Changsha 410128, China

Received:2016-05-16 Online:2016-11-20 Published:2016-11-18

摘要/Abstract

摘要：

试验旨在研究MAP3K5基因多态性与杜洛克猪生长、饲料利用性状的关联性。利用Illumina SNP60芯片测序结果比较发现了MAP3K5基因的4个SNPs位点（Ssc1：30769583 A>C；Ssc1：30781169 A>G；Ssc1：30940839 A>G；Ssc1：30962276 G>A）。统计获得MAP3K5基因的4个SNPs位点的基因型频率及等位基因频率，发现4个SNPs为低度－中度的多态突变位点。对MAP3K5基因多态性与生长、饲料利用性状的关联性进行分析，结果表明，杜洛克猪Ssc1：30769583 A>C位点CC基因型个体与AA基因型个体相比剩余采食量（RFI）性状降低了133.08 g/d（P<0.05）；Ssc1：30781169 A>G位点的GG基因型个体与AG基因型个体相比RFI性状降低了116.18 g/d（P<0.05），ADFI性状降低了0.23 kg/d（P<0.05）；Ssc1：30940839 A>G位点的GG基因型个体与AG基因型个体相比饲料转化率（FCR）性状降低了0.10%（P<0.05）；Ssc1：30962276 G>A位点的AA基因型个体与GG基因型个体相比ADG性状降低了0.04 kg/d（P<0.05）。试验初步认为MAP3K5基因4个SNPs位点的多态性对杜洛克猪的RFI、ADFI、FCR和ADG具有一定影响。

关键词: MAP3K5基因; 杜洛克猪; SNP; 剩余采食量; 饲料利用效率

Abstract:

This study was aimed to analyze the association of four SNPs of MAP3K5 gene with growth and feed efficiency related traits in Duroc populations. Four SNPs(Ssc1:30769583 A>C;Ssc1:30781169 A>G;Ssc1:30940839 A>G;Ssc1:30962276 G>A)of MAP3K5 gene in Duroc populations were obtained by porcine SNP60 BeadChip（Illumina). The genotype frequency and allele frequency of four SNPs of MAP3K5 gene were analyzed,the result showed that four SNPs were the low-moderate mutation SNPs.The polymorphism of MAP3K5 gene and its association with feed efficiency related traits in Duroc population were analyzed. The results indicated that CC genotype individuals in Ssc1:30769583 A>C had significant lower RFI（133.08 g/d）than AA genotype individuals（P<0.05);GG genotype individuals in Ssc1:30781169 A>G had significant lower RFI（116.18 g/d）and ADFI（0.23 kg/d）than AG genotype individuals（P<0.05);GG genotype individuals in Ssc1:30940839 A>G had significant lower FCR（0.10%）than AG genotype individuals（P<0.05);AA genotype individual in Ssc1:30962276 G>A had significant lower ADG（0.04 kg/d）than GG genotype individuals（P<0.05). All in all,the results suggested that four SNPs polymorphisms of MAP3K5 gene had the significant impact on RFI, ADFI, FCR and ADG traits in Duroc pigs.

Key words: MAP3K5 gene; Duroc pig; SNP; RFI; feed utilization efficiency

中图分类号:

S813.3

蒲蕾, 岳静伟, 张金山, 刘欣, 王立刚, 颜华, 侯欣华, 高红梅, 张恬, 张跃博, 武群清, 张龙超, 王立贤. 杜洛克猪MAP3K5基因多态位点与生长、饲料利用性状的关联分析[J]. 《中国畜牧兽医》, 2016, 43(11): 2982-2988.

PU Lei, YUE Jing-wei, ZHANG Jin-shan, LIU Xin, WANG Li-gang, YAN Hua, HOU Xin-hua, GAO Hong-mei, ZHANG Tian, ZHANG Yue-bo, WU Qun-qing, ZHANG Long-chao, WANG Li-xian. Association Analysis on MAP3K5 Gene Polymorphism Site with Growth and Feed Efficiency Related Traits in Duroc Pigs[J]. , 2016, 43(11): 2982-2988.

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杜洛克猪MAP3K5基因多态位点与生长、饲料利用性状的关联分析

Association Analysis on MAP3K5 Gene Polymorphism Site with Growth and Feed Efficiency Related Traits in Duroc Pigs

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