犊牛支原体肺炎继发牛A型多杀性巴氏杆菌感染的诊治

doi:10.16431/j.cnki.1671-7236.2015.11.035

›› 2015, Vol. 42 ›› Issue (11): 3065-3072.doi: 10.16431/j.cnki.1671-7236.2015.11.035

犊牛支原体肺炎继发牛A型多杀性巴氏杆菌感染的诊治

赖金伦¹, 寇明明¹, 刘玉辉¹, 朱习芳¹, 刘婧¹, 姜鹏¹, 陈颖钰^1,2, 胡长敏^1,2, 郭爱珍^1,2

1. 华中农业大学动物医学院, 武汉 430070;
2. 华中农业大学农业微生物学国家重点实验室, 武汉 430070

收稿日期:2015-04-08 出版日期:2015-11-20 发布日期:2015-11-26
通讯作者: 胡长敏, 郭爱珍 E-mail:hcm@mail.hzau.edu.cn;aizhen@mail.hzau.edu.cn
作者简介:赖金伦(1989-),男,广东化州人,硕士生,研究方向:奶牛乳腺炎,E-mail:jinlun@webmail.hzau.edu.cn
基金资助:
国家肉牛/牦牛产业技术体系(nycytx-38);国家自然科学基金项目(31101874);公益性行业(农业)科研专项(201003060);中央高校基本科研业务费专项资金资助项目(2662015PY054)

Diagnosis and Treatment of Mycoplasma bovis Pneumonia with Bovine Capsular Serotype A Pasteurella multocida Infection in Calf

LAI Jin-lun¹, KOU Ming-ming¹, LIU Yu-hui¹, ZHU Xi-fang¹, LIU Jing¹, JIANG Peng¹, CHEN Ying-yu^1,2, HU Chang-min^1,2, GUO Ai-zhen^1,2

1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;
2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

Received:2015-04-08 Online:2015-11-20 Published:2015-11-26

摘要/Abstract

摘要： 某奶牛场犊牛相继发生肺炎和关节炎,为确诊该牛场犊牛群发病的原因并提出防控方案,本试验剖检新生犊牛并采集病料,分别开展牛支原体及其他病原菌的分离培养、PCR鉴定及药敏分析;进行牛病毒性腹泻病毒、牛口蹄疫病毒和牛传染性鼻气管炎病毒PCR检测;制作犊牛肺脏组织病理切片并进行观察和评估。从犊牛肺脏组织分离到牛支原体和牛A型多杀性巴氏杆菌;牛病毒性腹泻病毒、牛口蹄疫病毒和牛传染性鼻气管炎病毒检测均为阴性;肺脏组织病理切片可见肺泡结构破坏、出血及大量炎性细胞浸润;药敏试验结果显示,牛支原体和牛A型多杀性巴氏杆菌分别对泰乐菌素和头孢唑啉敏感,但对青霉素、庆大霉素、林可霉素和氨苄西林均呈现耐药。该犊牛群确诊为牛支原体肺炎继发牛A型多杀性巴氏杆菌感染,采用泰乐菌素联合头孢唑啉肌肉注射,配合对症治疗和规范管理,有效控制了该场犊牛疾病。

关键词: 犊牛; 牛支原体肺炎; 牛A型多杀性巴氏杆菌; 诊断; 治疗

Abstract: The assay was aimed to clarify the reasons why pneumonia and arthritis had happened in a dairy farm,and provide effective control and treatment.A newborn calf was autopsied and the samples were collected.Mycoplasma bovis (M.bovis) and other pathogens were isolated and cultured,and PCR identification and drug sensitivity test were performed.Bovine viral diarrhea virus (BVDV),foot-and-mouth disease virus (FMDV) and infectious bovine rhinotracheitis virus (IBRV) were detected by PCR.Pathological slices from calf lung tissue were made to evaluate pathological changes.M.bovis and bovine capsular serotype A Pasteurella multocida (Pm A) were isolated,respectively.There were negative results for BVDV,FMDV and IBRV detection.Pathological slices of lung tissue showed damaged visible alveolar structure,bleeding and a large number of inflammatory cells infiltration.Drug sensitivity test results showed that M.bovis and Pm A were sensitive to tylosin and cefazolin,respectively,but resistance to penicillin,gentamicin,clindamycin and ampicillin.It was confirmed that M.bovis pneumonia with Pm A infection had happened.The disease in the farm was controlled by using tylosin and cefazolin at the same time.Furthermore,symptomatic treatment and standardized management should be carried.

Key words: calf; Mycoplasma bovis pneumonia; bovine capsular serotype A Pasteurella multocida; diagnosis; treatment

中图分类号:

S852.61⁺4

赖金伦, 寇明明, 刘玉辉, 朱习芳, 刘婧, 姜鹏, 陈颖钰, 胡长敏, 郭爱珍. 犊牛支原体肺炎继发牛A型多杀性巴氏杆菌感染的诊治[J]. , 2015, 42(11): 3065-3072.

LAI Jin-lun, KOU Ming-ming, LIU Yu-hui, ZHU Xi-fang, LIU Jing, JIANG Peng, CHEN Ying-yu, HU Chang-min, GUO Ai-zhen. Diagnosis and Treatment of Mycoplasma bovis Pneumonia with Bovine Capsular Serotype A Pasteurella multocida Infection in Calf[J]. , 2015, 42(11): 3065-3072.

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犊牛支原体肺炎继发牛A型多杀性巴氏杆菌感染的诊治

Diagnosis and Treatment of Mycoplasma bovis Pneumonia with Bovine Capsular Serotype A Pasteurella multocida Infection in Calf

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