营养调控对瘤胃及羊乳和羊肉中共轭亚油酸含量影响的研究进展

doi:10.16431/j.cnki.1671-7236.2015.07.019

摘要/Abstract

摘要： 共轭亚油酸(CLA)因具有抗癌、抗糖尿病及增强机体免疫力等功能而备受研究人员关注,近年来,针对备受消费者青睐的羊肉和羊奶等产品中CLA含量的研究越来越多。作者简述了反刍动物产品中CLA生物合成机制,并重点回顾了多种营养调控措施对瘤胃中CLA和十八碳烯酸积累的影响结果;进一步探讨了这几种营养调控方式对羊乳和羊肉中CLA含量的影响。分析认为,利用油脂底物和调控剂混合添加有利于加强瘤胃纤维菌群和原虫区系对CLA、十八碳烯酸的积累和过瘤胃率,并可在提高动物产品中CLA含量的同时,避免乳脂率下降。今后还需通过营养、分子生物学及生理学等角度全面揭示CLA合成机理,为生产富含CLA的动物产品提供参考。

关键词: 营养调控; 瘤胃; 羊肉; 羊奶; 共轭亚油酸

Abstract: Researcher pay attention to the conjugated linoleic acid (CLA) because of its function of anticancer,antidiabetic and immunological competence in the body.A lot of researches on contents of CLA in goat that enjoy high favor among people has been carried out in recent years.The article presents the biosynthsis process of CLA in ruminal,and focus on reviewing the result of many sdudies on the effects of the contents of CLA and vaccine acid in rumen;Further treat of the effects of nutrition regulation on CLA in goat's milk and mutton.Result show that the microflora and protozoa could increase the contents of CLA in rumen by using grease mixed of regulator,and increasing the contents of CLA in animal products at the same time,and avoiding to decline the contents of milk fat.we should reveal synthesis mechanism of CLA through nutrition,molecular biology,and physiology fully in the future,to provides a theoretical basis for producting CLA-enriched mutton and milk.

Key words: nutrition regulation; rumen; mutton; goat's milk; CLA

中图分类号:

S816

张志军, 赵芸君. 营养调控对瘤胃及羊乳和羊肉中共轭亚油酸含量影响的研究进展[J]. , 2015, 42(7): 1747-1754.

ZHANG Zhi-jun, ZHAO Yun-jun. Research Progress of Nutrition Regulation on Conjugated Linoleic Acid in Rumen, Goat's Milk and Mutton[J]. , 2015, 42(7): 1747-1754.

参考文献

[1] Pintus S,Murru E,Carta G,et al.Sheep cheese naturally enriched in α-linolenic,conjugated linoleic and vaccenic and vaccenic acids improves the lipid profile and reduces anandamide in the plasma of hypercholesterolaemic subjects[J].British Journal of Nutrition,2013,109(8):1453-1462.
[2] Ramirez G V,Villordo G I,Montenegro M,et al.Anticancer effects of bubaline functional milk with higher concentration of conjugated linoleic acid and omega-3 fatty acids[J].Buffalo Bulletin,2013,32(2):853-856.
[3] Lin X,Bo J,Oliver S A,et al.Dietary conjugated linoleic acid alters long chain polyunsaturated fatty acid metabolism in brain and liver of neonatal pigs[J].Journal of Nutritional Biochemistry,2011,22(11):1047-1054.
[4] 张瑞超,卜登攀,王加启,等.富含共轭亚油酸乳脂对小鼠免疫特性的影响[J].中国畜牧兽医,2006,33(6):30-32.
[5] Richter E K,Spangenberg J E,Klevenhusen F,et al.Stable carbon isotope composition of c9,t11-conjugated linoleic acid in cow's milk as related to dietary fatty acids[J].Lipids,2012,47(2):161-169.
[6] Schmidely P,Andrade P V D.Milk composition during duodenal infusion of conjugated linoleic acid in dairy goats fed diets with different C18:2/C18:1 ratios[J].Arquivo Brasileiro de Medicina Veterinariae Zootecnia,2011,63(5):1272-1276.
[7] Jin Y C,Li Z H,Hong Z S,et al.Conjugated linoleic acid synthesis-related protein proteasome subunit alpha 5 (PSMA5) is increased by t11-C18:1 vaccenic acid treatment in goat mammary tissue[J].Journal of Dairy Science,2012,95(8):4286-4297.
[8] 张玉枝,孔祥浩,朱晓萍,等.日粮精料水平对绵羊瘤胃内容物中亚油酸及其氢化产物组成的影响[J].中国畜牧杂志,2005,41(5):6-9.
[9] Szöllöskei G,Wagner L,Németh S,et al.In sacco studies of conjugated linoleic acid production from various oils in the rumen of sheep[J].Acta veterinaria Hungarica,2005,53(4):411-423.
[10] 金哲勇.日粮中添加胡麻油和苹果酸对延边黄牛瘤胃发酵特性及脂肪酸代谢的影响[D].延吉:延边大学,2012.
[11] Panatuk J,Uriyapongson S,Nawanukraw C,et al.Effect of dietary vegetable oil supplementation on C18 fatty acids and conjugated linoleic acid production:An in vitro fermentation study[J].Pakistan Journal of Nutrition,2013,12(6):516-520.
[12] AbuGhazaleh A A,Ishlak A.Effects of incremental amounts of fish oil on trans fatty acids and Butydvibrio bacteria in continuous culture fermenters[J].Joumal of Animal Physiology and Animal Nutrition,2014,98(2):271-278.
[13] Park H G,Heo W,Kim S B,et al.Production of conjugated linoleic acid (CLA) by Bifidobacterium breve LMC520 and its compatibility with CLA-producing rumen bacteria[J].Journal of Agricultural and Food Chemistry,2011,59(3):984-988.
[14] 杨波.乳酸菌生物转化共轭亚油酸的研究[D].无锡:江南大学,2014.
[15] Kurokawa Y,Shibata H,Tateno S,et al.Rumen fermentation,milk production and conjugated linoleic acid in the milk of cows fed high fiber diets added with dried distillers grains with solubles[J].Animal Science Journal,2013,84(2):116-120.
[16] Zapata S R,Gutiérrez B L A,Polanco E D,et al.Role of rumen ciliated protozoa in the synthesis of conjugated linoleic acid[J].Revista Colombiana de Ciencias Pecuarias,2012,25(1):135-149.
[17] Sultana H,Miyazawa K,Kanda S,et al.Fatty acid composition of ruminal bacteria and protozoa,and effect of defaunation on fatty acid profile in the rumen with special reference to conjugated linoleic acid in cattle[J].Animal Science Journal,2011,82(3):434-440.
[18] Zhu Z,Mao S,Zhu W.Effects of ruminal infusion of garlic oil on fermentation dynamics,fatty acid profile and abundance of baeteria involved in biohydrogenation in rumen of goats[J].Asian-Aust J Anim Sci,2012,25(7):962.
[19] 冉林武,黄克和,李林恩,等.富硒益生菌对山羊瘤胃发酵和瘤胃液脂肪酸含量的影响[J].南京农业大学学报,2011,34(5):99-104.
[20] 周薇,徐方华,陈娜,等.α-亚麻酸对瘤胃微生物体外发酵特性及生物氢化中间产物的影响[J].动物营养学报,2014,26(7):2018-2025.
[21] 尹珺伊.蓖麻油酸和茼蒿提取物对奶牛瘤胃中亚油酸生物氢化的影响[D].南京:南京农业大学,2013.
[22] 杨俊花,沈向真,王喜乐,等.葵花油和丙酮酸钙对山羊瘤胃消化代谢与瘤胃内反式油酸积累的影响[J].南京农业大学学报,2009,32(3):164-168.
[23] Hou J,Wang F,Wang Y,et al.Effects of vitamin E on the concentration of conjugated linoleic acids and accumulation of intermediates of ruminal biohydrogenation in vitro[J].Small Ruminant Research,2013,111(3):63-70.
[24] Daniel Z,Wynn R J,Salter A M,et al.Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues:The role of stearoyl-CoA desaturase[J].Journal of Animal Science,2004,82(3):747-758.
[25] Saichuer A,Kongmun P,Loongyai W.Guinea grass with meal concentrate on growth performance and conjugated linoleic acid (CLA) content in meat goat[A].Proceedings of the 50th Kasetsart University Annual Conference[C].2012.
[26] Cabiddu A,Addis M,Pinna G,et al.Effect of corn and beet pulp based concentrates on sheep milk and cheese fatty acid composition when fed Mediterranean fresh forages with particular reference to conjugated linoleic acid cis-9,trans-11[J].Animal Feed Science and Technology,2006,131(3):292-311.
[27] Guler G O,Aktumsek A.Effect of feeding regime on fatty acid composition and conjugated linoleic acid content of perirenal,omental and tail fat in Akkaraman lambs[J].African Journal of Biotechnology,2011,10(36):7099-7108.
[28] Allam S M,Ali M A,Mohamed A H,et al.Impact of different forms of oils on productive performance and conjugated linoleic acid content in goat's milk[J].Egyptian Journal of Nutrition and Feeds,2012,15(1):63-73.
[29] Modaresi J,Nasri M H F,Rashidi L,et al.Short communication:Effects of supplementation with pomegranate seed pulp on concentrations of conjugated linoleic acid and punicic acid in goat milk[J].Journal of Dairy Science,2011,94(8):4075-4080.
[30] Chen D,Qu X D,Fu J C,et al.Effect of sunflower seed on conjugated linoleic acid accumulation and relative enzymes gene expression of sheep[J].Journal of China Agricultural University,2012,17(1):110-118.
[31] 祁成年,王连群,雷红.日粮中添加棉籽对卡拉库尔羊肌肉和脂肪组织中共轭亚油酸含量的影响[J].畜牧与饲料科学,2012,33(9):57-58.
[32] Váradyová Z,Kišidayová S,Siroka P,et al.Comparison of fatty acid composition of bacterial and protozoal fractions in rumen fluid of sheep fed diet supplemented with sunflower,rapeseed and linseed oils[J].Animal Feed Science and Technology,2008,144(1):44-54.
[33] Jerónimo E,Alves S P,Martins S V,et al.Effect of sodium bentonite and vegetable oil blend supplementation on growth carcass quality and intramuscular fatty acid composition of lambs[J].Animal Feed Science and Technology,2010,158(3):136-145.
[34] Li X Z,Yan C G,Lee H G,et al.Influence of dietary plant oils on mammary lipogenic enzymes and the conjugated linoleic acid content of plasma and milk fat of lactating goats[J].Animal Feed Science and Technology,2012,174(1):26-35.
[35] Roy A,Mandal G P,Patra A K.Evaluating the performance,carcass traits and conjugated linoleic acid content in muscle and adipose tissues of Black Bengal goats fed soybean oil and sunflower oil[J].Animal Feed Science and Technology,2013,185(1):43-52.
[36] Szumacher-Strabel M,Cieslak A,Potkanski A,et al.The effects of different amounts and types of fat on the level of conjugated linoleic acid in the meat and milk of sheep[J].Journal of Animal and Feed Sciences,2001,10(2):103-108.
[37] Gagliostro G A,Rodriguez A,Pellegrini P A,et al.Effects of fish oil or sunflower plus fish oil supplementation on conjugated linoleic acid (CLA) and omega 3 fatty acids in goat milk[J].Revista Argentina de Produccion Animal,2006,26(2):71-87.
[38] Demirel G,Wood J D,Enser M.Conjugated linoleic acid content of the lamb muscle and liver fed different supplements[J].Small Ruminant Research,2004,53(1):23-28.
[39] Chow T T,Fievez V,Moloney A P,et al.Effect of fish oil on in vitro rumen lipolysis,apparent biohydrogenation of linoleic and linolenic acid and accumulation of biohydrogenation intermediates[J].Animal Feed Science and Technology,2004,117(1):1-12.
[40] 杨光,王加启,卜登攀,等.十二指肠灌注18碳脂肪酸对泌乳奶牛机体免疫力的影响[J].中国畜牧兽医,2010,37(11):5-8.
[41] 罗军,单翠燕,王海滨,等.共轭亚油酸对奶山羊公羔肉用性能以及肌肉和脂肪中脂肪酸组成的影响[J].动物营养学报,2010,22(3):709-716.
[42] 慕向东.共轭亚油酸对羔羊免疫反应和肉品质的影响[D].北京:中国农业大学,2007.
[43] Baldin M,Gama M A S,Dresch R,et al.A rumen unprotected conjugated linoleic acid supplement inhibits milk fat synthesis and improves energy balance in lactating goats[J].Journal of Animal Science,2013,91(7):3305-3314.
[44] Fernandes D,Gama M A S,Ribeiro C,et al.Milk fat depression and energy balance in stall-fed dairy goats supplemented with increasing doses of conjugated linoleic acid methyl esters[J].Animal,2014,8(4):587-595.
[45] Borys B,Borys A,Pajak J J.The fatty acid profile of meat of suckling lambs from ewes fed rapeseed and linseed[J].Journal of Animal and Feed Sciences,2005,14:223-226.
[46] Tsiplakou E,Zervas G.The effect of dietary inclusion of olive tree leaves and grape marc on the content of conjugated linoleic acid and vaccenic acid in the milk of dairy sheep and goats[J].Journal of Dairy Research,2008,75(3):270-278.
[47] Romano R,Masucci F,Giordano A,et al.Effect of tomato by-products in the diet of Comisana sheep on composition and conjugated linoleic acid content of milk fat[J].International Dairy Journal,2010,20(12):858-862.
[48] Liu K,Ge S,Luo H,et al.Effects of dietary vitamin E on muscle vitamin E and fatty acid content in Aohan fine-wool sheep[J].Journal of Animal Science and Biotechnology,2013,4(1):21.
[49] 韩正强,沈向真,杨晓静,等.日粮添加半胱胺和海南霉素对波杂一代山羊肌内共轭亚油酸含量的影响[J].华中农业大学学报,2007,26(1):71-75.
[50] 刘志刚.十八碳植物油和丙酸前体对奶山羊乳脂脂肪酸合成的影响[D].延吉:延边大学,2013.
[51] Du Toit A N,Einkamerer O B,Hugo A,et al.The effect of dietary lipid saturation and monensin-Na on the CLA content of lambs' meat[J].Journal of Animal Science,2013,43:75-80.
[52] Li X Z,Yan C G,Long R J,et al.Conjugated linoleic acid in rumen fluid and milk fat,and methane emission of lactating goats fed a soybean oil-based diet supplemented with sodium bicarbonate and monensin[J].Asian-Australasian Journal of Animal Sciences,2009,22(11):1521-1530.