饲粮纤维水平对育肥羔羊瘤胃微生物组成及多样性的影响

doi:10.16431/j.cnki.1671-7236.2021.04.012

摘要/Abstract

摘要： 试验旨在研究提高饲粮纤维水平对育肥羔羊瘤胃微生物组成及多样性的影响。试验选择健康无病、体重相近的2月龄杜湖杂交公羔，按照体重随机分为2组，每组38只，分别饲喂不同的饲粮，对照组（LW_CK）饲粮为精料+苜蓿，处理组（LW_EG）为精料+苜蓿+全株玉米青贮+花生秧；试验期150 d，其中预试期20 d，正试期130 d，试验结束后，每组随机选择5只羔羊屠宰取瘤胃内容物，通过16S rDNA高通量测序技术分析其微生物多样性及结构变化。结果表明，处理组羔羊瘤胃中细菌多样性显著高于对照组（P<0.05）。在门水平上，处理组羔羊瘤胃中优势菌门为拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes），对照组优势菌门为拟杆菌门和变形菌门（Proteobacteria）。提高纤维水平后显著增加了育肥羔羊瘤胃中厚壁菌门的相对丰度（P<0.05）；显著降低了变形菌门的相对丰度（P<0.05）。在属水平上，对照组与处理组羔羊瘤胃菌群共鉴定出195个属，处理组优势菌属为普雷沃氏菌属（Prevotella_1）和解琥珀酸菌属（Succiniclasticum），对照组优势菌属为琥珀酸弧菌属（Succinivibrionaceae）和普雷沃氏菌属（Prevotella_7）。提高饲粮纤维水平，增加了育肥羔羊瘤胃中解琥珀酸弧菌属、密螺旋体属（Treponema_2）、疣微菌属（Ruminococcaceae）、月形单胞菌属（Selenomonas_1）的相对丰度，显著降低了琥珀酸弧菌属相对丰度（P<0.05）。综上所述，适量提高饲粮中纤维水平对育肥羔羊瘤胃微生物菌群结构有一定影响，能够促进部分纤维降解菌的增殖，有利于育肥羔羊的瘤胃发酵和养分消化利用。

关键词: 瘤胃微生物; 多样性; 育肥羔羊; 16S rDNA

Abstract: This experiment was conducted to investigate the effects of dietary crude fiber level enhancement on rumen microbial composition and diversity of fattening lambs.In this study,healthy and disease-free male lambs were selected.All lambs with similar weight at the age of 2 months were randomly divided into two groups according to the body weight,38 males in each group were fed with different diets.The diets of control group were composed of concentrate and alfalfa hay,while the diets of the treatment group were composed of whole corn silage and peanut seedling based on control group diet.The period of trial lasted 150 d,including 20 d in the preliminary phase.After the end of the experiment,5 lambs from each group were slaughtered,and rumen contents were collected for the measurement of microbial diversity and structural changes by 16S rDNA high-throughput sequencing technology.The results showed that the bacterial diversity in the rumen of the treated lambs was significantly higher than that of the control group (P<0.05).At the level of phylum,the dominant bacteria in the rumen of the lambs in the treatment group were Bacteroidetes and Firmicutes,while the dominant bacteria in the control group were Bacteroidetes and Proteobacteria.After the addition of fiber,the relative abundance of Firmicutes in the rumen of the fattening lambs was significantly increased (P<0.05),the relative abundance of Proteobacteria was significantly reduced (P<0.05).At the level of genus,195 genera were identified from the rumen bacteria of the control group and the treatment group,and the dominant bacteria in the treatment group were Prevotella_1 and Succiniclasticum,while the dominant bacteria in the control group were Succinivibrionaceae and Prevotella_7.The relative abundance of Succiniclasticum,Treponema_2,Ruminococcaceae and Selenomonas_1 in the rumen of fattening lambs was increased by increasing the dietary fiber level,and the relative abundance of Succinivibrionaceae was significantly reduced (P<0.05).Therefore,the increase of dietary fiber level appropriately had a certain effect on the structure of rumen microbial flora of fattening lambs,which could promote the proliferation of some fiber-degrading bacteria,and was beneficial to rumen fermentation and nutrient digestion and utilization of fattening lambs.

Key words: rumen microorganisms; diversity; fattening lamb; 16S rDNA

中图分类号:

S826

李希, 毛杨毅, 罗惠娣, 赵鹏, 李俊, 张丽, 李春艳, 王宏浩. 饲粮纤维水平对育肥羔羊瘤胃微生物组成及多样性的影响[J]. 中国畜牧兽医, 2021, 48(4): 1251-1263.

LI Xi, MAO Yangyi, LUO Huidi, ZHAO Peng, LI Jun, ZHANG Li, LI Chunyan, WANG Honghao. Effects of Dietary Crude Fiber Level on Rumen Microbial Composition and Diversity of Fattening Lambs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1251-1263.

参考文献

[1] 刘玉峰,丛玉艳.饲粮碳水化合物对羔羊瘤胃发育影响的研究进展[J].动物营养学报,2020,32(2):566-570. LIU Y F,CONG Y Y.Research advances of effects of dietary carbohydrate on rumen development in lambs[J].Chinese Journal of Animal Nutrition,2020,32(2):566-570.(in Chinese)
[2] 田可,柳君辉,董国忠.早期瘤胃微生物区系发育及其调控[J].动物营养学报,2018,30(12):4828-4834. TIAN K,LIU J H,DONG G Z.Development and manipulation of rumen microbiota during early-life[J].Chinese Journal of Animal Nutrition,2018,30(12):4828-4834.(in Chinese)
[3] REY M,ENJALBERT F,COMBES S,et al.Establishment of ruminal bacterial community in dairy calves from birth to weaning is sequential[J].Journal of Applied Microbiology,2014,116(2):245-257.
[4] 张牧州,郝小燕,项斌伟,等.玉米皮和大豆皮组合替代玉米和玉米秸秆对育肥羊生长性能和瘤胃代谢的影响[J].动物营养学报,2020,32(3):1-10. ZHANG M Z,HAO X Y,XIANG B W,et al.Effects of replacing corn and corn straw with corn husk and soybean hulls combination on growth performance and rumen metabolism in fattening sheep[J].Chinese Journal of Animal Nutrition,2020,32(3):1-10.(in Chinese)
[5] 解彪,张乃锋,张春香,等.粗饲料对幼龄反刍动物瘤胃发育的影响及其作用机制[J].动物营养学报,2018,30(4):1245-1252. XIE B,ZHANG N F,ZHANG C X,et al.Effects of forage on rumen development in young ruminants and its mechanisms[J].Chinese Journal of Animal Nutrition,2018,30(4):1245-1252.(in Chinese)
[6] SADET-BOURGETEAU S,MARTIN C,MORGAVI D P.Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets[J].Veterinary Microbiology,2010,146(1-2):98-104.
[7] 杨宏波,刘红,占今舜,等.不同精粗比颗粒饲料对断奶公犊牛瘤胃发酵参数和微生物的影响[J].草业学报,2015,24(12):131-138. YANG H B,LIU H,ZHAN J S,et al.Effects of granule feed with different precision and coarse ratio on rumen fermentation parameters and microorganisms in weaned male calves[J].Acta Pratacultural Science,2015,24(12):131-138.(in Chinese)
[8] WANG W M,LI C,LI F D,et al.Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs[J].Scientific Reports,2016,6(1):32479.
[9] 刘玉洁.高谷物饲粮对山羊瘤胃微生物区系和瘤胃、血清、肝脏中代谢物组成的影响[D].南京:南京农业大学,2015. LIU Y J.Effects of high-grain diet on the rumen microflora and metabolite composition in rumen,serum and liver of goats[D].Nanjing:Nanjing Agricultural University,2015.(in Chinese)
[10] 李岚捷,成述儒,刁其玉,等.不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响[J].畜牧兽医学报,2017,48(12):2347-2357. LI L J,CHENG S R,DIAO Q Y,et al.Effects of diets with different NFC/NDF levels on the rumen fermentation parameters and bacterial community in male calves[J].Acta Veterinaria et Zootechnica Sinica,2017,48(12):2347-2357.(in Chinese)
[11] 魏德泳,朱伟云,毛胜勇.日粮不同NFC/NDF比对山羊瘤胃发酵与瘤胃微生物区系结构的影响[J].中国农业科学,2012,45(7):1392-1398. WEI D Y,ZHU W Y,MAO S Y.Effects of different dietary NFC/NDF ratios on the ruminal fermentation and the changes of the rumen microbial community of goats[J].Scientia Agricultura Sinica,2012,45(7):1392-1398.(in Chinese)
[12] 郭凯,唐梦琪,霍倩倩,等.不同蛋白水平饲粮对犊牛瘤胃微生物区系多样性及组成的影响[J].家畜生态学报,2019,40(11):22-28. GUO K,TANG M Q,HUO Q Q,et al.Effects of diets with different protein level on rumen bacterial community in calves[J].Acta Ecologiae Animalis Domastici,2019,40(11):22-28.(in Chinese)
[13] 韩旭峰.日龄、日粮精粗比对陕北白绒山羊瘤胃微生物区系影响的研究[D].杨凌:西北农林科技大学,2015. HAN X F.Study on the influence of the ratio of day age and grain essence to grain diameter on the rumen microflora of Bairong goats in Northern Shaanxi[D].Yangling:Northwest A&F University,2015.(in Chinese)
[14] 林波,梁辛,李丽莉,等.饲粮精粗比对泌乳水牛瘤胃细菌和甲烷菌区系的影响[J].动物营养学报,2016,28(10):3101-3109. LIN B,LIANG X,LI L L,et al.Dietary forage to concentrate ratio affects ruminal bacterial and methanogen community composition of water buffaloes[J].Chinese Journal of Animal Nutrition,2016,28(10):3101-3109.(in Chinese)
[15] PITTA D W,KUMAR S,VEICCHARELLI B,et al.Bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of Mehshana buffalo (Bubalus bubalis) using 16S pyrotags[J].Anaerobe,2014,25:31-41.
[16] 程雅婷,鲜凌瑾,武秋申,等.不同纤维源饲粮对四川白鹅肠道微生物菌群结构的影响[J].中国兽医学报,2020,40(8):1632-1640. CHENG Y T,XIAN L J,WU Q S,et al.Effects of different dietary fiber sources on intestinal microflora structure of Sichuan White goose[J].Chinese Journal of Veterinary Science,2020,40(8):1632-1640.(in Chinese)
[17] 魏园,赵晨旭,李心慰,等.不同粗纤维源日粮对梅花鹿瘤胃微生物区系的影响[J].中国兽医学报,2018,38(1):217-229. WEI Y,ZHAO C X,LI X W,et al.Effect of different crude fiber source diets on rumen microflora of Sika deer[J].Chinese Journal of Veterinary Science,2018,38(1):217-229.(in Chinese)
[18] KONG Y H,HE M L,MCALISTER T,et al.Quantitative fluorescence in situ hybridization of microbial communities in the rumens of cattle fed different diets[J].Applied and Environmental Microbiology,2010,76(20):6933-6938.
[19] 董春晓,吕佳颖,牛骁麟,等.粗饲料来源对育肥湖羊瘤胃微生物区系及肌肉脂肪酸组成的影响[J].草业科学,2019,36(11):2926-2936. DONG C X,LYU J Y,NIU X L,et al.Effects of dietary roughage sources on the rumen microflora and muscle fatty acids in finishing Hu lambs[J].Pratacultural Science,2019,36(11):2926-2936.(in Chinese)
[20] PEI C X,LIU Q,DONG C S,et al. Diversity and abundance of the bacterial 16S rRNA gene sequences in forestomach of alpacas (Lama pacos) and sheep (Ovis aries)[J].Anaerobe,2010,16(4):426-432.
[21] HOOK S E,STEELE M A,NORTHWOOD K S,et al.Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cows[J].FEMS Microbiology Ecology,2011,78(2):275-284.
[22] 胡安海.日粮peNDF水平对山羊生产性能、能量代谢及瘤胃微生物群落结构的影响[D].成都:四川农业大学,2019. HU A H.Effects of dietary peNDF levels on the performance,energy metabolism and rumen microbial community structure of goats[D].Chengdu:Sichuan Agricultural University,2019.(in Chinese)
[23] 李与琦,阳建华,张涛,等.日粮中添加不同比例微贮棉秆对湖羊瘤胃微生物区系的影响[J].微生物学报,2020,60(8):1592-1604. LI Y Q,YANG J H,ZHANG T,et al.Effect of different proportions fermented cotton stalk intake on rumen microflora of lambs[J].Acta Microbiologica Sinica,2020,60(8):1592-1604.(in Chinese)
[24] 吕小康.不同饲粮对20~60日龄山羊羔羊瘤胃发育的影响[D].北京:中国农业科学院,2019. LYU X K.Effects of different diets on rumen development of 20-60 day-old goat kids[D].Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
[25] 李娜,张洁,郭婷婷,等.基于内转录间隔区测序分析不同饲养方式对滩羊羔羊瘤胃真菌组成及多样性的影响[J].动物营养学报,2020,32(2):1-11. LI N,ZHANG J,GUO T T,et al.Effects of different feeding methods on composition and diversity of rumen fungi in Tan sheep based on interstitial region sequencing[J].Chinese Journal of Animal Nutrition,2020,32(2):1-11.(in Chinese)
[26] STANTON T B,CANALEPAROLA E.Treponema bryantii sp.nov.a rumen spirochete that interacts with cellulolytic bacteria[J].Archives of Microbiology,1980,127(2):145-156.
[27] 李海琴,贾建磊,陈倩,等.饲粮蛋白质水平对断奶羔羊瘤胃组织形态及微生物群落结构与功能的影响[J].动物营养学报,2020,32(12):1-10. LI H Q,JIA J L,CHEN Q,et al.Effects of dietary protein level on rumen morphology,microbial community structure and function of weaned lambs[J].Chinese Journal of Animal Nutrition,2020,32(12):1-10.(in Chinese)
[28] WEIMER P J,LOPEZ-GUISA J M,FRENCH A D.Effect of cellulose fine structure on kinetics of its digestion by mixed ruminal microorganisms in vitro[J].Applied and Environmental Microbiology,1990,56(8):2421-2429.
[29] KRAUSE D O,RUSSELL J B.An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination[J].Applied and Environmental Microbiology,1996,62(3):815-821.
[30] 周瑞,赵生国,刘立山,等.饲粮中燕麦干草含量对绵羊瘤胃液pH及微生物区系的影响[J].动物营养学报,2016,28(5):1589-1597. ZHOU R,ZHAO S G,LIU L S,et al.Effects of dried oat hay content in diet on rumen fluid pH and microflora of sheep[J].Chinese Journal of Animal Nutrition,2016,28(5):1589-1597.(in Chinese)
[31] 杨硕,韦玥瑞,梁天,等.放牧及补饲对内蒙古绒山羊瘤胃微生物数量的影响[J].饲料研究,2020,9:21-24. YANG S,WEI Y R,LIANG T,et al.Effect of grazing and supplementary feeding on the number of rumen microorganisms in Inner Mongolia cashmere goats[J].Feed Research,2020,9:21-24.(in Chinese)
[32] 董瑞阳.粗饲料组合对泌乳牛与干奶牛甲烷产量、瘤胃发酵模式及微生物菌群的影响[D].郑州:河南农业大学,2014. DONG R Y.Effect of roughage combinations on methane production,rumen fermentation pattern and microbial population of lactating cows and dry cows[D].Zhenzhou:Henan Agricultural University,2014.(in Chinese)
[33] 李萍,梁辛,韦升菊,等.饲粮精粗比对后备母水牛瘤胃发酵参数和纤维降解菌数的影响[J].畜牧与兽医,2015,47(7):36-40. LI P,LIANG X,WEI S J,et al.Effects of dietary forage to concentrate ratio on rumen fermentation parameters and fiber degrading bacteria number of reserve female buffalo[J].Animal Husbandry & Veterinary Medicine,2015,47(7):36-40.(in Chinese)
[34] 普宣宣.饲粮NFC/NDF对卡拉库尔羊消化、瘤胃发酵及瘤胃微生物的影响[D].阿拉尔:塔里木大学,2020. PU X X.Effects of dietary NFC/NDF ratio on digestion,rumen fermentation and the rumen microorganism in Karakul sheep[D].Alaer:Tarim University,2020.(in Chinese)
[35] SARO C,RANILLA M J,TEJIDO M L,et al.Influence of forage type in the diet of sheep on rumen microbiota and fermentation characteristics[J].Livestock Science,2014,160(2):52-59.
[36] ZHANG R Y,ZHU W Y,ZHU W,et al.Effect of dietary forage sources on rumen microbiota,rumen fermentation and biogenic amines in dairy cows[J].Journal of the Science of Food and Agriculture,2014,94(9):1886-1895.
[37] HUO W J,ZHU W Y,MAO S Y.Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats[J].World Journal of Microbiology & Biotechnology,2014,30(2):669-680.
[38] 胡丹丹,郭婷婷,李娜,等.以16S rDNA高通量测序法研究果寡糖对奶牛瘤胃菌群结构及多样性的影响[J].中国畜牧兽医,2018,45(10):2761-2771. HU D D,GUO T T,LI N,et al.Effect of fructooligosaccharide on the structure and diversity of rumen bacterial community in dairy cow by 16S rDNA sequencing technology[J].China Animal Husbandry & Veterinary Medicine,2018,45(10):2761-2771.(in Chinese)
[39] 谢骁.低质粗饲料日粮干预对湖羊瘤胃发酵和微生物菌群的影响[D].杭州:浙江大学,2018. XIE X.Effects of dietary intervention on rumen fermentation and microbiota in sheep fed low quality forage[D].Hangzhou:Zhejiang University,2018.(in Chinese)
[40] PURUSHE J,FOUTS D E,MORRISON M,et al.Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii:Insights into their environ-mental niche[J].Microbial Ecology,2010,60(4):721-729.