不同精粗比饲粮对绵羊瘤胃体外发酵和共轭亚油酸产量的影响

doi:10.16431/j.cnki.1671-7236.2022.05.012

摘要/Abstract

摘要： 【目的】在添加2%豆油基础上研究不同精粗比饲粮对绵羊瘤胃体外发酵和共轭亚油酸产量的影响。【方法】试验共设7个饲粮精粗比组(精粗比分别为2∶8、3∶7、4∶6、5∶5、6∶4、7∶3、8∶2)，称取300 mg不同精粗比饲粮于尼龙袋中，并添加2%豆油于玻璃发酵管中，吸取30 mL培养液，体外发酵48 h，每种精粗比设4个平行，并进行2次重复试验。【结果】 ①4∶6组乙酸浓度最高且极显著高于其他各组(P<0.01)。戊酸浓度随精粗比比值增加先增加后降低，4∶6组显著高于6∶4组和7∶3组(P<0.05)。4∶6组总挥发性脂肪酸浓度显著高于2∶8组、3∶7组、6∶4组、7∶3组(P<0.05)。其他处理组之间丙酸、丁酸、异丁酸、异戊酸浓度和乙酸∶丙酸比例均无显著差异(P>0.05)。8∶2组瘤胃液pH最高。②4∶6组干物质降解率显著高于3∶7和5∶5组(P<0.05)。4∶6组甲烷产量最低，且极显著低于6∶4组(P<0.01)。6∶4组总产气量显著高于2∶8、3∶7和5∶5组(P<0.05)，但与4∶6组无显著差异(P>0.05)。③氨态氮浓度随精粗比增加先增加后降低，4∶6组氨态氮浓度最高，极显著高于2∶8和8∶2组(P<0.01)，显著高于3∶7和6∶4组(P<0.05)。4∶6组微生物蛋白浓度最高且极显著高于其他各组(P<0.01)。④共轭亚油酸浓度在各组间无显著差异(P>0.05)。【结论】添加2%豆油基础上饲粮精粗比为4∶6水平时最有利于绵羊瘤胃发酵。

关键词: 精粗比; 大豆油; 体外发酵; 共轭亚油酸

Abstract: 【Objective】 On the basis of adding 2% soybean oil,this experiment was conducted to investigate the effects of concentrate to forage ratio on ruminal fermentation and conjugated linoleic acid production of sheep in vitro.【Method】 Rumen fluid from sheep was used as the inoculum,and 7 levels of dietary concentrate to forage ratio (concentrate to forage ratio was 2∶8,3∶7,4∶6,5∶5,6∶4,7∶3,8∶2 respectively) were used as the substrate,2% soybean oil was added into glass fermentation tubes during the in vitro 48 h fermentation.The trail was repeated twice with four parallels in each group.【Result】 ①The concentration of acetic acid in level 4∶6 was the highest and significantly higher than those with others (P<0.01).The concentration of valeric acid increased firstly and then decreased with the increase of concentrate to forage ratio,and the concentration was hightest in level 4∶6.The concentration of total volatile acid in level 4∶6 was significantly higher than that in levels 2∶8,3∶7,6∶4 and 7∶3 (P<0.05).There was no significant difference in the concentration of propionic acid,butyric acid,isobutyric acid,isovaleric acid and the ratio of acetic acid to propionic acid between other treatment levels (P>0.05).pH value was highest at level 8∶2.②The dry matter degradation rate of level 4∶6 was significantly higher than levels 3∶7 and 5∶5 (P<0.05).The production of methane in level 4∶6 was the lowest and significantly lower than that in level 6∶4 (P<0.01).Gas production of level 6∶4 was higher than levels 2∶8,3∶7 and 5∶5 (P<0.05),but there was no significant difference with level 4∶6 (P>0.05).③The concentration of ammonia nitrogen increased first and then decreased,which the highest point located in level 4∶6,extremely significantly higher than that in levels 2∶8 and 8∶2 (P<0.01),significantly higher than that in levels 3∶7 and 6∶4 (P<0.05).The concentrations of microbial protein and acetic acid in level 4∶6 were the highest and significantly higher than those with others (P<0.01).④There was no significant difference in the concentration of conjugated linoleic acid in each group (P>0.05).【Conclusion】 On the basis of supplemented 2% soybean oil,the level of 4∶6 of dietary concentrate to forage ratio was the best value for ruminal fermentation and dry matter degradation of sheep in vitro.

Key words: concentrate to forage ratio; soybean oil; in vitro fermentation; conjugated linoleic acid

中图分类号:

S816.79

田希雅, 孙阳, 钮慧晶, 于天力, 何振莲, 夏呈强, 裴彩霞. 不同精粗比饲粮对绵羊瘤胃体外发酵和共轭亚油酸产量的影响[J]. 中国畜牧兽医, 2022, 49(5): 1707-1714.

TIAN Xiya, SUN Yang, NIU Huijing, YU Tianli, HE Zhenlian, XIA Chengqiang, PEI Caixia. Effects of Dietary with Different Concentrate to Forage Ratio on Ruminal Fermentation and Conjugated Linoleic Acid Production of Sheep in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1707-1714.

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