动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定

doi:10.16431/j.cnki.1671-7236.2015.02.035

›› 2015, Vol. 42 ›› Issue (2): 459-466.doi: 10.16431/j.cnki.1671-7236.2015.02.035

动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定

黄凯, 陈素娟, 黄骏, 杨林, 鞠勇, 朱相儒, 孙志豪, 彭大新

扬州大学兽医学院, 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009

收稿日期:2014-08-19 出版日期:2015-02-20 发布日期:2015-02-13
通讯作者: 彭大新 E-mail:daxinpeng@yahoo.com
作者简介:黄凯(1990-),男,江苏靖江人,硕士生,研究方向:动物传染病学,E-mail:905651669@qq.com;陈素娟(1976-),女,江苏靖江人,博士,副教授,研究方向:动物病原微生物的致病机理和免疫机理研究及新型疫苗研制,E-mail:1259562123@qq.com
基金资助:
公益行业(农业)科研专项(201303044);江苏省家禽疫病防控工程技术研究中心(BM2013450);江苏高校优势学科建设工程

Analysis of Antibiotic Resistance of Salmonella Isolated from Animals and Identification of its Florfenicol Resistant Gene

HUANG Kai, CHEN Su-juan, HUANG Jun, YANG Lin, JU Yong, ZHU Xiang-ru, SUN Zhi-hao, PENG Da-xin

Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

Received:2014-08-19 Online:2015-02-20 Published:2015-02-13

摘要/Abstract

摘要： 为了解动物沙门氏菌的流行情况和药物敏感性及氟苯尼考耐药株的耐药基因分布,本试验对临床上疑似患沙门氏菌病的病料进行病原分离和细菌的多重PCR鉴定;采用K-B法测定分离株对23种抗菌药物的敏感性;选择氟苯尼考耐药菌株扩增floR、fexA、fexB、cfr和pexA基因。结果显示,共鉴定出61株沙门氏菌,其中肠炎沙门氏菌10株,鸡白痢沙门氏菌12株,鼠伤寒沙门氏菌39株。所有菌株对青霉素、红霉素、万古霉素耐药,90.16%对6种及6种以上抗菌药耐药。floR基因广泛存在于鼠伤寒沙门氏菌氟苯尼考耐药菌株中(8/12,66.67%),未发现其他耐药基因。研究结果表明鼠伤寒沙门氏菌是鹅源分离株中的优势血清型;floR基因主要介导沙门氏菌对氟苯尼考耐药性,但可能还存在其他机制。

关键词: 沙门氏菌; 血清型; 耐药性; floR基因

Abstract: The assay was aimed to investigate prevalent Salmonella strains from animal,their antibiotic susceptibility,and distribution of florfenicol resistant genes in Salmonella isolates with resistance to florfenicol.Pathogens were isolated from clinical samples with suspected salmonellosis,and identified by mPCR.Antibiotic resistance to 23 antibiotics was determined by antibiotic susceptibility test according to the K-B method.The isolates with resistance to florfenicol were chosen to PCR amplification for floR,fexA,fexB,cfr and pexA genes.The results showed 61 Salmonella strains were isolated,and the numbers of S.Enteritidis,S.Pullorum and S.Typhimurium were 10,12 and 39,respectively.All strains were resistant to penicillin,erythromycin and vancomycin,and 90.16% of the isolates were resistant to six or more than six actibiotics.The floR genes were detected in 8 out of 12 florfenicol-resisitant S.Typhimurium strains,and no other genes were detected.These data indicated that S.Typhimurium was the dominant serotype of Salmonella isolated from geese.The floR gene played a major role in its florfenicol resistance.However,it might exist other antibiotic resistance mechanism.

Key words: Salmonella; serotype; antibiotic resistance; floR gene

中图分类号:

S852.61⁺4

黄凯, 陈素娟, 黄骏, 杨林, 鞠勇, 朱相儒, 孙志豪, 彭大新. 动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J]. , 2015, 42(2): 459-466.

HUANG Kai, CHEN Su-juan, HUANG Jun, YANG Lin, JU Yong, ZHU Xiang-ru, SUN Zhi-hao, PENG Da-xin. Analysis of Antibiotic Resistance of Salmonella Isolated from Animals and Identification of its Florfenicol Resistant Gene[J]. , 2015, 42(2): 459-466.

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动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定

Analysis of Antibiotic Resistance of Salmonella Isolated from Animals and Identification of its Florfenicol Resistant Gene

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