DHRS4基因在猪骨骼肌中的表达及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.01.016

摘要/Abstract

摘要： 为探讨猪DHRS4基因在骨骼肌中的表达及其与生长性状的相关性，本研究分析了DHRS4基因在长白猪出生后30、180和300 d不同类型骨骼肌中的表达，并在蓝思白猪资源群体中筛选DHRS4基因的多态性位点，进一步通过Sequenom SNP分型技术对DHRS4基因进行基因分型，分析了DHRS4基因多态性位点与猪生长性状（料重比、平均日增重和平均背膘厚）的相关性。结果显示，DHRS4基因在30、180和300 d长白猪的胸肌、腿肌和背肌中均有表达，在胸肌和腿肌中，DHRS4基因在30 d的表达显著或极显著高于180和300 d（P<0.05；P<0.01），而在背肌的不同发育时期中，其表达水平没有显著差异（P>0.05）。此外，本研究在蓝思白猪资源群体中找到了3个位于DHRS4基因上的单核苷酸多态性位点：rs334250151、rs342446613和rs326982309。关联分析结果表明，rs334250151位点与料重比呈显著相关，该位点AA基因型个体的料重比显著低于TT基因型个体（P<0.05）。研究揭示，DHRS4基因可能参与猪的骨骼肌发育，rs334250151位点可以作为一个新的分子标记应用于猪生长性状的遗传改良中。

关键词: DHRS4基因; SNP; 生长性状; 骨骼肌

Abstract: In order to investigate the expression of porcine DHRS4 gene in skeletal muscle and its association with growth traits,this study analyzed the expression of DHRS4 gene in different types of skeletal muscles in Landrace pigs at 30,180 and 300 d.The single nucleotide polymorphism (SNP) of DHRS4 gene in Lansi White pig resource population were identified and genotyped by the Sequenom genotyping platform.The association of the SNP in DHRS4 gene was analyzed with growth traits,including feed gain ratio,average daily gain and average backfat thickness.The results showed that DHRS4 gene was expressed in breast,leg and dorsal muscles of Landrace pigs at 30,180 and 300 d.In breast and leg muscles,the expression of DHRS4 was significantly or extremely significantly higher at 30 d than that at 180 and 300 d (P<0.05;P<0.01),but there was no significant difference of dorsal muscles at different developmental stages (P>0.05).In addition,this study found 3 SNPs of DHRS4 gene in Lansi White pig resource population:rs334250151,rs342446613 and rs326982309.The association analysis results showed that rs334250151 loci was significantly associated with feed gain ratio,and the feed gain ratio of AA genotype individuals was significantly lower than that of TT genotype individuals (P<0.05).This results reveals that DHRS4 gene might be involved in porcine skeletal muscle development,and rs334250151 loci could be used as a new molecular marker for genetic improvement of growth traits in pig.

Key words: DHRS4 gene; SNP; growth traits; skeletal muscle

中图分类号:

S813.3

张垒霞, 杨言昭, 李文通, 刘颖, 周荣, 杨亚岚, 李奎. DHRS4基因在猪骨骼肌中的表达及其与生长性状的关联分析[J]. 中国畜牧兽医, 2020, 47(1): 131-139.

ZHANG Leixia, YANG Yanzhao, LI Wentong, LIU Ying, ZHOU Rong, YANG Yalan, LI Kui. Expression of Porcine DHRS4 Gene in Skeletal Muscle and Its Association with Growth Traits[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 131-139.

参考文献

[1] 王克君.基于多重关联分析鉴定猪生长性状相关基因及其功能验证[D].北京:中国农业大学,2017. WANG K J.Identification of growth-related genes based on multi-association analysis and their potential gene function verification in pig[D].Beijing:China Agricultural University,2017.(in Chinese)
[2] YANG Y L,ZHOU R,LI K.Future livestock breeding:Precision breeding based on multiomics information and population personalization[J].Journal of Integrative Agriculture,2017,16(12):2784-2791.
[3] 李梦云,郑萍,李婉涛,等.PRKAG3基因在不同品种猪不同生长阶段骨骼肌中的表达差异及其表达量与肉质的关系[J].动物营养学报,2017,29(5):1661-1669. LI M Y,ZHENG P,LI W T,et al.The expression difference of PRKAG3 gene in skeletal muscle at different growth stages for different breeds of pigs and the relationship between PRKAG3 gene expression level and meat quality[J].Journal of Animal Nutrition,2017,29(5):1661-1669.(in Chinese)
[4] MILAN D,JEON J T,LOOFT C,et al.A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle[J].Science,2000,288(5469):1248-1251.
[5] DVORAKOVA V,BARTENSCHLAGER H,STRATIL A,et al.Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses[J].Genetics Selection Evolution,2012,44(1):13.
[6] ZHOU R,YANG Y L,LIU Y,et al.Association of CYP19A1 gene polymorphisms with reproductive traits in pigs[J].Journal of Integrative Agriculture,2017,16(7):1558-1565.
[7] 赵聪哲,罗晓彤,李兆华,等.大白猪MC4R、CDC16基因多态性及其与生长性状的关联分析[J].中国畜牧兽医,2019,46(3):792-799. ZHAO C Z,LUO X T,LI Z H,et al.Association between polymorphism of MC4R and CDC16 genes and growth traits in Large White pigs[J].China Animal Husbandry & Veterinary Medicine,2019,46(3):792-799.(in Chinese)
[8] 宋志芳,于国生,解佑志,等.分子遗传标记技术及其在动物育种中的研究进展[J].猪业科学,2017,34(2):113-115. SONG Z F,YU G S,XIE Y Z,et al.Molecular genetic marker technology and its research progress in animal breeding[J].Pig Science,2017,34(2):113-115.(in Chinese)
[9] HUANG D Y,ICHIKAWA Y.Purification and characterization of a novel cytosolic NADP(H)-dependent retinol oxidoreductase from rabbit liver[J].Biochimica et Biophysica Acta,1997,1338(1):47-59.
[10] SU Z J,LI R,SONG X H,et al.Identification of a novel isoform of DHRS4 protein with a nuclear localization signal[J].Gene,2012,494(2):161-167.
[11] 李蕊.NRDR及其选择性剪接亚型的表达、抗体制备和亚细胞定位[D].汕头:汕头大学,2007. LI R.Expression,antibody preparation and subcellular localization of NRDR and its isoform[D].Shantou:Shantou University,2007.(in Chinese)
[12] 苏中静,陈海滨,宋旭红,等.DHRS4L1基因选择性剪接亚型的克隆及分析[J].解剖科学进展,2011,17(1):63-66. SU Z J,CHEN H B,SONG X H,et al.Cloning and analyzing the alternative spliced variants of DHRS4L1 gene[J].Advances in Anatomical Science,2011,17(1):63-66.(in Chinese)
[13] HUANG H F,LI M T,POGACH L M,et al.Messenger ribonucleic acid of rat testicular retinoic acid receptors:Developmental pattern,cellular distribution,and testosterone effect[J].Biology of Reproduction,1994,51(3):541-550.
[14] LE BOUFFANT R,GUERQUIN M J,DUQUENNE C,et al.Meiosis initiation in the human ovary requires intrinsic retinoic acid synthesis[J].Human Reproduction,2010,25(10):2579-2590.
[15] LI Q,FOOTE M,CHEN J.Effects of histone deacetylase inhibitor valproic acid on skeletal myocyte development[J].Scientific Reports,2014,4:7207.
[16] WONG J,MEHTA V,VORONOVA A,et al.Beta-catenin is essential for efficient in vitro premyogenic mesoderm formation but can be partially compensated by retinoic acid signalling[J].PLoS One,2013,8(2):e57501.
[17] 张艳敏,刘颖,李文通,等.日照大白猪胴体性能和肉品质的测定及MyHC基因在肌肉组织中的表达分析[J].农业生物技术学报,2018,26(4):616-625. ZHANG Y M,LIU Y,LI W T,et al.Carcass performance and meat quality analysis of Rizhao Large White pigs (Sus scrofa) and the expression of MyHC genes in muscle tissues[J].Journal of Agricultural Biotechnology,2018,26(4):616-625.(in Chinese)
[18] 刘楠,阮进学,杨述林.猪肌肉发育调控和高瘦肉率基因改良猪的研究进展[J].农业生物技术学报,2012,20(8):955-964. LIU N,RUAN J X,YANG S L.Regulation of porcine muscle development and research progress of improved pigs with high lean meat percentage[J].Journal of Agricultural Biotechnology,2012,20(8):955-964.(in Chinese)
[19] LEFAUCHEUR L,ECOLAN P,PLANTARD L,et al.New insights into muscle fiber types in the pig[J].Journal of Histochemistry & Cytochemistry,2002,50(5):719-730.
[20] 宋佳巍,高光,吴伟,等.鹅肌纤维组织学特性及MyoG基因mRNA表达研究[J].吉林农业大学学报,2013,35(1):89-93. SONG J W,GAO G,WU W,et al.Study on expression of MyoG mRNA and histological characteristics of goose muscle fiber[J].Journal of Jilin Agricultural University,2013,35(1):89-93.(in Chinese)
[21] 何宝龙.乌珠穆沁羊骨骼肌肌纤维组织学特性及MyHC表达量变化分析[D].呼和浩特:内蒙古农业大学,2017. HE B L.Analysis of muscle fiber characteristics and MyHC expression in the skeletal muscle of Wuzhumuqin sheep[D].Hohhot:Inner Mongolia Agricultural University,2017.(in Chinese)
[22] 杨晓静,赵茹茜,陈杰,等.猪背最长肌肌纤维类型的发育性变化及其品种和性别特点[J].中国兽医学报,2005,25(1):89-94. YANG X J,ZHAO R Q,CHEN J,et al.The developmental changes of myofibre types in LD muscle of erhualian and Large White pigs[J].Chinese Journal of Veterinary Science,2005,25(1):89-94.(in Chinese)
[23] 朱春红,徐文娟,陈文峰,等.高邮鸭和金定鸭发育早期骨骼肌MyoG和Myf6基因的表达分析[J].西北农林科技大学学报(自然科学版),2013,41(12):14-20. ZHU C H,XU W J,CHEN W F,et al.Expression of MyoG and Myf6 mRNA in skeletal muscle of Gaoyou duck and Jinding duck in early development stage[J].Journal of Northwest Agricultural and Forestry University (Natural Science Edition),2013,41(12):14-20.(in Chinese)
[24] BRUNE R M,BARD J B,DUBREUIL C,et al.A three-dimensional model of the mouse at embryonic day 9[J].Developmental Biology,1999,216(2):457-468.
[25] LIU Y,YANG Y L,LI W T,et al.NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs[J].Molecular Reproduction and Development,2019,86(1):63-74.
[26] HWANG J H,AN S M,KWON S G,et al.Associations of the polymorphisms in DHRS4,SERPING1,and APOR genes with postmortem pH in Berkshire pigs[J].Animal Biotechnology,2017,28(4):288-293.