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Table of Content
20 August 2007, Volume 34 Issue 8
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动物营养与饲料科学
Research Progress on Molecular Mechanism of Glucose Absorption and Regulation in Animal’s Intestine
LI Yong;CAI Huiyi;LIU Guohua
2007, 34(8): 5-9.
Abstract
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1402
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Glucose is one of essential nutrients and important energy source to animals. It plays critical role in metabolism of animal. Hitherto, the mechanism of glucose absorption has been clarified and accepted by most of specialists. But the mechanism to regulate its absorption was still unclear because of the limits of research methods and conditions. This article reviews the molecular mechanism of glucose absorption and regulation by diet, development and hormone etc.
The Study of Microecological Products on the Caecal Flora of Heilongjiang Zi Geese
DONG Wei;ZHANG Yujie;LIN Fenghai;LIU Xiangjiang;ZHANG Lei;HUANG Yulan;YANG Jun
2007, 34(8): 20-22.
Abstract
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1972
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120 Heilongjing Zi geese were divided into three groups(control and Ⅰ,Ⅱ groups),every group was forty. The control group was base feed,The group Ⅰand Ⅱ were appended 1.5 ml/kg、1.0 ml/kg microecological products daily for 90 d in base feed. The result indicated: Compared with the control,the number of lactobacillus in the experimentsⅠ,Ⅱ caecum were
all more significant(P<0.05), the number of Enterobacillus and Salmonella were all much fewer (P<0.05);The same experimental group had no significiant difference (P>0.05).The experiments daily consuming feed and fresh propotion had significiant difference compared with the control (P<0.05). The experiment showed that the microecological Pruducts had the function of regulating the important flora with impact on caecum, and increased the immune function and growth performance.
Effect of Feeding with Fermentation Protein on Layers
JIN Yufen
2007, 34(8): 24-25.
Abstract
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1296
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The fermentation protein was fermented by 70% rapeseed Meals and 30%cottonseed meal, and which was added to the diet of the layers for substituting the soybean meal or the fishmeal. The results showed that the test group’s layer’s egg produce rate was reduced 2.22% and 2.21%,and the average egg weight was increased 2.31g and 2.03g,while the inputoutput ratio was increased 3.6% and 1.68% respectively compared with the control group.
遗传繁育
Comparison of CTC Staining and in Vitro Fertilization among Different Ovine Sperm Capacitation Methods
LIU Lieqin;LIU Chousheng;GUO Yong;NI Hemin;ZHANG Hong;YANG Feige;LIU Yunhai
2007, 34(8): 31-34.
Abstract
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1928
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In this experiment, the discrepancy was compared among the “Swim-up” , Induction by A23187, Percoll and Incubation after Percoll methods for ovine sperm capacitation in vitro with chlortetracycline fluorescence (CTC) staining and in vitro fertilization. The results showed that the rates of sperm capacitation and the cleavage treated by Swim-up method were higher than the others. It might be suggested that the Swim-up method is exactly suitable for the application of in vitro fertilization of sheep.
The Principles and Applications Of EPD Estimated
LI He;CHEN Wenli;LIU Xuehong
2007, 34(8): 35-40.
Abstract
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1666
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Expected progeny difference (EPD) is a sort parameter which provides estimation of the genetic value of an animal as a parent based on theory of BLUP. EPD value is a half of estimated breeding value, which is understood easily. It directly reflects breeding value from animal. Specifically, EPD is main index for genetic merit differences between two individuals from the same breed. So it can predict performance differences between their offspring if they are mated to animals having average genetic merit. EPDs are calculated for birth, growth, maternal, and carcass traits and more traits with animal performances. EPD has been broadly applied in sire evaluation. The results of EPD estimation for individual are easily been published or announced on internet and (or) intranet. The producer can select their favorite bulls according to their own requirements. The paper will give details about the principles and applications of EPD.
Genetic Diversity among Mink Based on the RAMP Markers
WANG Hongmei;BAI Xiujuan
2007, 34(8): 41-44.
Abstract
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1960
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Relationships among three mustela vison species which were standardpitchy mink、sapphire mink、coffee mink were analyzed by RAMP Markers. The results showed distinct genetic differences were present among the species, and polymorphic percentage was very high. Fourteen primer combinations produced 81 bands, 75 bands were polymorphic. The polymorphic percentage was 92.59%, 4~8 bands were amplified by each primer combinations, with an average of 5.78 bands. Polymorphic bands percent 、Shannon’s Information index、Nei’s (1973) gene diversity showed high genetic diversity. In population genetic differentiation analysis, Nei’s genetic distance and the coefficient of gene differentiation were used and the results showed that the genetic differentiation degree between populations was lower and the hereditary variation primarily came from within population.
Effects of Neuroendocrine Factors on Expression of GLNR mRNA in MonolayerPrimary Cultured Neonatal Calf Hepatocytes in Vitro
ZHANG Yonghong;SUN Yucheng;LIU Guowen;GAO Yan;WANG Xueying;LI Xiaobing;WANG Zhe
2007, 34(8): 45-48.
Abstract
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1338
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In order to investigate the regulation role of neuroendocrine factors Insulin(In), glucagon(GLN) and leptin(LEP) in hepatocyte glycometabolism and lipometabolism of dairy cows, effects of In, GLN and LEP on expression of neonatal calf glucagon receptor（GLNR）mRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.In results, the production of GLNR mRNA increased(P<0.01) along with increase of levels of In;and when glucagon in the culture media increased gradually, the production of GLNR mRNA decreased (P<0.01). Along with increase of leptin in the medium, the production of GLNR increased firstly and then decreased (P<0.01). These results demonstrated that In, GLN and LEP in the medium can directly regulate the expression of GLNR mRNA in the neonatal calf hepatocytes in vitro.
The Research Progress of Histological Traits of Muscle Fibre in Pig
GUO Yunyan;WANG Lixian;CHENG Duxue;YAN Hua
2007, 34(8): 49-52.
Abstract
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1420
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Intensive research demonstrate that muscle fibre characteristics are thought to be important factors influencing meat quality.In order to provide preference for the further research，in the following article ，the classification and development of MF，the research of MF candidate gene were summarized.
生物技术
The Influence of Donor Cell Treatments on the Development of Nuclear Transfer Embryos of Bovine
LIANG Suli;LI Xiangchen;BAI Xiuyun;WANG Youzhu;GUAN Weijun
2007, 34(8): 53-57.
Abstract
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1605
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In order to study the influence of Donor Cells Treatments on the Development of nuclear transfer embryos of Bovine. The influence of cryopreservative and fresh fibroblast cells、different Centrifugal speed of donor cell、different serum starvation and passage time of cryopreservative fibroblast cells after thawing were studied. The cleavage and blastocyst rate of nuclear transfer embryos derived from cryopreservative and fresh fibroblast cells were not significantly different（P>0.05）; The cleavage and blastocyst rate of nuclear transfer embryos derived from donor cell treated with centrifugal speed 1500 r/min was significantly lower than other groups;The blastocysts that obtained from 3～5 d serum starvation is highter than other group. The cleavage and blastocyst rate of nuclear transfer embryos from passage 8 times after thawing is lowest compared with passage 2 and 5 times. Different treatment of donor cell have impotent effects for nuclear transfer embryos.
生理生化
Measurements of Common Physiological Value of Sichuan White Swan
WANG Yangming;HUANG Yongfu;ZENG Xiu;ZHANG Tingke;CHENG Bigang;CHEN Shijiang;LUO Yi
2007, 34(8): 62-63.
Abstract
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1812
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The main physiological value of 10 male swans, 10 female swans were measured in Rongchang county which is the main production area of Sichuan white swan, results as following: average body temperature, respiration rate, pulse, hemoglobin density, red blood number, white blood number of adult male swan and female swan are 41.2℃, 11.2 t/min,15 t/min; 99.7 t/min, 91.7 t/min; 191 g/L, 168 g/L; 2.55 10E12/L, 2.35 10E12/L; 98 10E9/L, 96.7 10E9/L respectively and its blood rheological parameter etc.
The Technique Steps of Raise Dairy Cow Dry Matter Intake
CHEN Chengzhen;WANG Zhe
2007, 34(8): 64-66.
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1829
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The periparturient dairy cows, especially for highly produce dairy cows, are in the state of negatively energy balanced condition generally,which leads to degrade of production trait and metabolic diseases such as ketosis and fatty liver.The dry matter intake of dairy cows is increased by the breeding, improvement feed formula and useing additives,which raises production and improved health status of dairy cows for some extent.The paper describes the significances and the influencing factors of dairy cows dry matter intake,and also draws off some technique steps to increase dairy cows dry matter intake.
奶业专栏
Effects of Different Supplementary Concentration Se-yeast in Dairy Ration on Digestibility
ZHANG Lijuan;YAO Juan;TAN Bin;XU Zhongyun
2007, 34(8): 66-68.
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1271
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8 cows were used in a 4×4 Latin squre design to study the effect of different supplementary concentration Seyeast on apparent digestibility. control:Na2SeO3, Se 0.3 mg/kg DM; Seyeast, the Se concentration were 0.3、0.45、0.6 mg/kg DM. The result is that: compare to control, 0.45 mg/kg DM could enhance the apparent digestibility of OM, NDF,ADF, HC,CEL, Ca, P（P<0.01）and CP（P<0.05）; compare to other treated groups, the cows with 0.45 mg/kg also had higher nutrient apparent digestibility.
疾病防治
A Survey of Wild Boars in Guangxi for Infection of Classical Swine Fever
CHEN Fenglian;MA Ling;PAN Hanshi;HUANG Hongmei;SUN Jianhua;CHEN Zhongwei;WU Jianmin
2007, 34(8): 75-77.
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1661
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RT-PCR and ELISA detected CSFV in wild boars in this article. A pair of specific primers were designed and synthesized, then RT-PCR was used to detect 28 spleens and lymph tissues of wild boars from5 cities in Guangxi province, and the results were all positive; meanwhile, ELISA was used to detected these samples ,the results were also all positive. This result indicates that wild boars from 5cities in Guangxi province haven’t infected CSFV.
Investigation and Treatment of Siberian Tigers and African Lions Ascariasis in Zhengzhou Zoo
JIAN Fuchun;ZHU Jinxin;ZHAO Jinfeng;LI Tongyi;LI Dezhong;ZHONG Longxian;NING Changshen
2007, 34(8): 78-81.
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1236
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The sheather’s sugar and the saturated brine flotation technique were used in this test. 7 Siberian tigers and 8 lions in Zhengzhou Zoo were surveyed on infection of ascariasis. The result indicated that the infection rate was 86.7%, the average EPG was 4880, and the highest was 12400. Albendazole tablets was used to treat the positive animal at 5-10 mg/kg.bw (feed in food) and continue 7 days, the eegs decreaased remarkably, the result showed the rat of infection descent from 86.7% to 38.5%, ascarid eggs slip was 100%,and couldn’t be detected EPG by MacMaster’s method, this shown that therapy effect was fine.
Rangoon Creeper Extracts the Thing Expelling Parasite Experiment to Affecting the Pig Ascarid Small Rat
YANG Jisheng;XIAOXiao;YANG Meilan;XU Xueqian
2007, 34(8): 81-82.
Abstract
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1391
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Choose kind (km) of small 50 Kunming white mice, half and half divides into five set , divides because Rangoon creeper extracts thing thickness diversity among them three set, the others one is to contrast a group, one is a blank space group , abstract the thing expelling parasite result , the experiment lasting for one months having been in progress for probing into Rangoon creeper. Result indicates a thing , Rangoon creeper picks up to have very good getting rid of effect of pig ascarid.
In Vitro Susceptibilities of Escherichia Coli Isolated from Sick Chickens to Cefquinome and its Comparator
DU Xiangdang;MO Juan;ZHANG Sumei;LIU Jianhua;LI Xinsheng
2007, 34(8): 83-84.
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1348
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Specimens were collected from sick or dead chickens from 8 cities in northern Henan province. 63 suspected strains were purified and then identified by biochemical test as E. coli which was lethal to chickens by animal challenging test. The results of bacterial susceptibility experiments by the broth microdilution test indicated that the tested bacteria presented 33.3%，46%，63.5% and 98.4% resistance to cefquinome, ceftiofur, florfenicol and sarafloxacin, respectively. The resistant condition of the chicken E. coli to several newer veterinary drugs solely used in animals should not be neglected.
A Research Report on the Once-only-treatment and Nursing of Equus Tetanus
SONG Yinzhang;WANG Jinyi;LIAN Xuezhao;HAN Mujun
2007, 34(8): 87-88.
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1712
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The once-only-treatment and nursing of equus tetanus could be difined to infuse the equus with 500 ml 5% glucose combined separately with 300,000-350,000 units of tetanus antitoxin and 80-100 ml Urotropine. Then, the sick animal is put into a warm, tranquilized place with a horse-suspending equipment and a rope by being given water and fed in order not to be stimulated by something bad. Water should be put before the sick animal while it is nursed well. Better effect could be obtained when the animal is given cicada soup 300-400 g cooked from cicada molts 4 times a day for 4 days. The results from the past twenty years reveal that the cured efficacy of 131 examples is 100% with the once-only-treatment and careful nursing.
流行病防治
Study on Diagnosis of Swine Toxoplasmosis and Its Clinical Pathological
LIU Yonghong;LIU Yuehuan;WANG Fenglong;WANG Jinling;HAN Chunhua;REN Ke;YANG Longfeng
2007, 34(8): 100-103.
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1782
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The swine disease was researched with epidemiology and studied with clinical pathology, at the same time, it was tested with serology and PCR. The results showed that the cause of the swine disease is toxoplasma. The clinical symptoms are high hot staying up, anhelation, body surface in purple, diarrhea comes after abstraction, circulation discoordination, finally exhaust and die. The pathological characteristics are pneumonedema, interstitial pneumonia, necrotic splenitis, local necrotic hepatitis and lymph, nonpurulent neuraxitis and interstitial nephritis, and so on.
兽医临床
Investigation of Pathogenic Bacteria in Weaning Brash Piglets in Field
YANG Xue;GUO Dingzong;ZHU Haibao;WU Qingru;LI Wenbo;QIAN Guosheng
2007, 34(8): 114-116.
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1814
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In hubei province,there were 180 piglets diarrheaed in certain pig field. The dead weaning brash piglets were autopsy and observed that some tissue occured cheese pathological change. And plasma membrane had different level bleeding point all over the body. We found that bowels wall was thickness and elasticity decreased. Then selected randomly serious weaning brash piglets were 10 and normal piglets were 3. From their content of intestinal tract, 7 strain bacteria were isolated and cultured. In order biochemistry identited they were salmonella typhisuis, Bacterium aerogenes, Escherichia, pneumonia bacillus, Bacterium vulgare of Proteus, Shigella. The percentage in piglets diarrheaed were 87.5%,57.1%,100%,28.6%,28.6%,14.3%,28.6%.Through pathogenicity testing on mouse the difference of pathopoiesis were 100%,.60%,10%,20%,40%,60%,80%. It was concluded that salmonella typhis was the main pathogenic bacteria in the field.
畜禽产品
Studies on Carcass and Meat Quality Performance and Its Correlativity of Finishing Pigs
GUO Jianfeng;WU Ying;ZHANG Yin;WANG Jiying
2007, 34(8): 128-131.
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1550
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Carcass performance and meat quality of 31 finishing pigs of 92~109 kg body weigh stage were studied. And the changes of carcass and meat quality as body weight changed were analyzed .The objectives were to investigate appropriate slaughter weight of finishing pigs. Results indicated that lean percent of the stage of 92~95 kg slaughter weight was the higher than that of others ,it was 61.17%, increased by 4.83%（P>0.05）,11.83%（P<0.05）and 8.84％（P<0.05）as compared with the other three groups, respectively. When the slaughter weight was 107~109 kg , the meat quality was fine, pH was 6.07, meat color and marbling score were 3.13 and 3.33, intramuscular fat content of muscles was 3.89%, water loss was18.91% . The correlation degree of carcass traits and meat traits had strength and weakness, correlation coefficients had positive and negative. The same traits had stronger positive correlation, the adverse traits had stronger negative correlation .Comprehensive evaluation showed that the reasonable slaughter weight stage of finishing pigs was 92～95 kg when lean percent was stressed, 107~109 kg was selected when meat quality was stressed, and 95~98 kg was selected when carcass performance and meat quality were stressed.
经验交流
Advances in the Research of Mammalian Defensins
LONG Jing;DU Lixin
2007, 34(8): 137-141.
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1249
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Defensins are peptidic components of the innate immune system of plants and animals. In mammals, defensins have evolved to have a central function in the host defense properties of granulocytic leukocytes, mucosal surfaces, skin and epithelial. This review focuses on the region and distribution of defensin, the characteristic of molecular structure, biological activity, functions and application of mammalian defensins.
Studies on Population Genetics Structure of Super-fine Breed NuclearPopulation of Chinese Merino(Xinjiang Army Westland Reclamation Model) County in Xinjiang
ZENG Xiancun;SHI Guoqing;JIA Bin;WANG Jianhua;HE Qihong;ZHANG Yongsheng;PENG Linze;YU Zhiyong
2007, 34(8): 142-145.
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1760
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The Polymorphisms distribution of 10 microsatellite Loci were determined in 154 samples of Superfine Breed nuclear Population in Chinese Merino(xinjiang army westland reclamation model) county. Polymorphism information content(PIC), effective number of alleles(E), gene heterozygosity(H) and Rate of homozygote of alleles (Rh) for all microsatellite loci were calculated. The resusts showed the 8 of 10 microsatellite Loci were highly polymorphic, and BMS772 and BM6506 was medium polymorphic ; The mean PIC, H,E, and Rh were 0.5848、0.6333、3.0315、0.4841 respectively, this population have high genetic diversity and medium rate of homozygote of alleles ; and just OARAE57 locus obeyd HardyWeinberg balance equilibrium (P>0.05).
