Abstract: In order to study the influence of Donor Cells Treatments on the Development of nuclear transfer embryos of Bovine. The influence of cryopreservative and fresh fibroblast cells、different Centrifugal speed of donor cell、different serum starvation and passage time of cryopreservative fibroblast cells after thawing were studied. The cleavage and blastocyst rate of nuclear transfer embryos derived from cryopreservative and fresh fibroblast cells were not significantly different（P>0.05）; The cleavage and blastocyst rate of nuclear transfer embryos derived from donor cell treated with centrifugal speed 1500 r/min was significantly lower than other groups;The blastocysts that obtained from 3～5 d serum starvation is highter than other group. The cleavage and blastocyst rate of nuclear transfer embryos from passage 8 times after thawing is lowest compared with passage 2 and 5 times. Different treatment of donor cell have impotent effects for nuclear transfer embryos.

Key words: donor cell; bovine; nuclear transfer; embryo of NT