添加胰岛素-转铁蛋白-亚硒酸钠对牛体外胚胎生产、低温保存及常规冷冻效果的影响

doi:10.16431/j.cnki.1671-7236.2023.10.021

Abstract

Abstract: 【Objective】 This study was aimed to evaluate the effect of adding insulin-transferrin-sodium selenite (ITS) to production,4 ℃ hypothermic storage and programmed freezing system of bovine in vitro embryos,so as to improve the production and preservation effect of in vitro bovine embryos via ITS.【Method】 After in vitro fertilization of bovine oocytes derived from slaughterhouse ovaries,the following experiments were carried out:① An experiment to study the effect of adding ITS to anaphase culture medium M2 on blastocyst rate,with M2+ITS and M2-control (without ITS,control) groups. ② An experiment to further investigate the effect of adding ITS to prophase culture medium M1 on early embryonic development,with M1+M2+ITS(both M1 and M2 with ITS addition) and M1-control (without ITS addition but M2,control) groups. ③ An experiment to further evaluate the effect of ITS in improving embryonic development under three gases culture conditions,with ITS (with ITS) and control (without ITS) groups.The cleavage rate,day-7 blastocyst rate,and total blastocyst rate were calculated for each of the above groups.④ The day-7 bovine embryos were stored at 4 ℃ for 24 h by M2 storage solution with ITS (Hypo-ITS group),or day-7 in vitro embryos in frozen solution with ITS were frozen by programmed procedure (Cryo-ITS group),with Hypo-control (without ITS) and Cryo-control (without ITS) group,and the survival and hatching rates of the recovered and thawed embryos were counted,respectively.【Result】 Compared with control group,the day-7 blastocyst rates of M2+ITS and M1+M2+ITS groups were significantly increased (P＜0.05);Under the three gas culture conditions,the day-7 blastocyst rate in ITS group was also significantly increased (P＜0.05).There was no significant difference of the survival rate between Hypo-ITS and Hypo-control groups (P＞0.05),but the hatching rate of Hypo-ITS group was significantly higher than that of Hypo-control group (P＜0.05).There was no significant difference of the survival and hatching rates of programmed freezing between Cryo-ITS and Cryo-control groups (P＞0.05).【Conclusion】 ITS could be used as additive and hypo-protectant to improve the effect of bovine in vitro embryo production and hypo-preservation.However,the addition of ITS in the freezing solution did not significantly improve the programmed freezing effect of bovine in vitro embryos.

Key words: bovine in vitro embryo; insulin-transferrin-sodium selenite(ITS); hypothermic storage; routine programmed freezing

CLC Number:

S814.6

ZHANG Haobo, ZHAO Shanjiang, WANG Huan, XU Xi, FENG Zhiqiang, WANG Wanjie, GONG Jianfei, PANG Yunwei, DU Weihua, ZHU Huabin. Effects of Addition of Insulin-Transferrin-Sodium Selenite on Effect of Production, Hypothermic Storage and Routine Freezing of Bovine in vitro Embryos[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4077-4084.

References

[1] ALESHIRE S L,OSTEEN K G,MAXSON W S,et al.Localization of transferrin and its receptor in ovarian follicular cells:Morphologic studies in relation to follicular development[J].Fertility and Sterility,1989,51(3):444-449.
[2] KHAN I,MESALAM A,SONG S H,et al.Supplementation of insulin-transferrin-sodium selenite in culture medium improves the hypothermic storage of bovine embryos produced in vitro[J].Theriogenology,2020,152:147-155.
[3] CORDOVA B,MORATO R,IZQUIERDO D,et al.Effect of the addition of insulin-transferrin-selenium and/or L-ascorbic acid to the in vitro maturation of prepubertal bovine oocytes on cytoplasmic maturation and embryo development[J].Theriogenology,2010,74(8):1341-1348.
[4] MESALAM A,LEE K L,KHAN I,et al.A combination of bovine serum albumin with insulin-transferrin-sodium selenite and/or epidermal growth factor as alternatives to fetal bovine serum in culture medium improves bovine embryo quality and trophoblast invasion by induction of matrix metalloproteinases[J].Reproduction,Fertility,and Development,2019,31(2):333-346.
[5] DAS Z C,GUPTA M K,UHM S J,et al.Supplementation of insulin-transferrin-selenium to embryo culture medium improves the in vitro development of pig embryos[J].Zygote,2014,22(3):411-418.
[6] SMITH S R,GHOSH M C,OLLIVIERRE-WILSON H,et al.Complete loss of iron regulatory proteins 1 and 2 prevents viability of murine zygotes beyond the blastocyst stage of embryonic development[J].Blood Cells,Molecules & Diseases,2006,36(2):283-287.
[7] GEORGE F,DANIAUX C,GENICOT G,et al.Set up of a serum-free culture system for bovine embryos:Embryo development and quality before and after transient transfer[J].Theriogenology,2008,69(5):612-623.
[8] NASR-ESFAHANI M H,JOHNSON M H.How does transferrin overcome the in vitro block to development of the mouse preimplantation embryo?[J].Journal of Reproduction and Fertility,1992,96(1):41-48.
[9] 安全利.褪黑素在牛卵母细胞体外成熟过程中的作用研究[D].杨凌:西北农林科技大学,2019. AN Q L.Study on the role of melatonin in the in vitro maturation of bovine oocytes[D].Yangling:Northwest A&F University,2019.(in Chinese)
[10] 侯银仙.牛体外胚胎生产技术研究进展[J].畜牧兽医科技信息,2013,9:8-10. HOU Y X.Research progress of bovine in vitro embryo production technology[J]. Science and Technology Information of Animal Husbandry and Veterinary Science,2013,9:8-10.(in Chinese)
[11] CHANG T,ZHAO G.Ice inhibition for cryopreservation:Materials,strategies,and challenges[J].Advanced Science,2021,8(6):2002425.
[12] FERRE L B,KJELLAND M E,TAIYEB A M,et al.Recent progress in bovine in vitro-derived embryo cryotolerance:Impact of in vitro culture systems,advances in cryopreservation and future considerations[J].Reproduction in Domestic Animals,2020,55(6):659-676.
[13] CHOWDHURY M M R,MESALAM A,KHAN I,et al.Improved developmental competence in embryos treated with lycopene during in vitro culture system[J].Molecular Reproduction and Development,2018,85(1):46-61.
[14] YOUNIS A I,ROOKS B,KHAN S,et al.The effects of antifreeze peptide Ⅲ (AFP) and insulin transferrin selenium (ITS) on cryopreservation of chimpanzee (Pan troglodytes) spermatozoa[J].Journal of Andrology,1998,19(2):207-214.
[15] YOUNG L E,SINCLAIR K D,WILMUT I.Large offspring syndrome in cattle and sheep[J].Reviews of Reproduction,1998,3(3):155-163.
[16] ABE H,YAMASHITA S,SATOH T,et al.Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum-containing media[J].Molecular Reproduction and Development,2002,61(1):57-66.
[17] SEIDEL G E JR.Modifying oocytes and embryos to improve their cryopreservation[J].Theriogenology,2006,65(1):228-235.
[18] NAGASHIMA H,KASHIWAZAKI N,ASHMAN R J,et al.Removal of cytoplasmic lipid enhances the tolerance of porcine embryos to chilling[J].Reproductive Biology,1994,51(4):618-622.
[19] JULIE,WEATHERS D,SAMUEL,et al.Estimation of weight and lipid composition in preimplantation embryos from jersey and beef breeds of cattle[J].Open Journal of Veterinary Medicine,2014,4:261-266.
[20] CROCCO M C,KELMANSKY D M,MARIANO M I.Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro,during developmental days 1 to 4?[J].Journal of Assisted Reproduction and Genetics,2013,30(10):1377-1388.
[21] ISKANDAR H,SONJAYA H,YUSUF M.Effect of Adding insulin transferrin selenium (ITS) in the medium on maturation and fertilization rates of Bali cattle oocytes[J].Jurnal Ilmu Ternak dan Veteriner,2019,24(3):95.
[22] MESALAM A,KHAN I,LEE K L,et al.2-methoxystypandrone improves in vitro-produced bovine embryo quality through inhibition of IKBKB[J].Theriogenology,2017,99:10-20.
[23] DUQUE RODRIGUEZ M,CITTADINI C O,TEPLITZ G M,et al.Canine IVM with SOF medium,insulin-transferrin-selenium,and low O(₂) tension improves oocyte meiotic competence and decreases reactive oxygen species levels[J].Frontiers in Cell and Developmental Biology,2021,9:694889.
[24] WIDJIATI W,LUQMAN E M,TEHUPURING B C,et al.Effectivity of insulin transferrin selenium and bovine serum albumin addition on in vitro culture medium on fertilization and blastocyst rate of mice (Mus musculus)[J].Journal of International Dental & Medical Research,2017,10(3):1080-1083.
[25] MONDAL S,MOR A,REDDY I J,et al.Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation,fertilization and embryo development in sheep[J].Brazilian Archives of Biology and Technology,2015,58(4):521-525.
[26] VAN SOOM A,VANDAELE L,GOOSSENS K,et al.Gamete origin in relation to early embryo development[J].Theriogenology,2007,68(Suppl 1):S131-S137.
[27] YU Y,DING C,WANG E,et al.Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis[J].Reproduction,2007,133(5):947-954.
[28] HASLER J F,HENDERSON W B,HURTGEN P J,et al.Production,freezing and transfer of bovine IVF embryos and subsequent calving results[J].Theriogenology,1995,43(1):141-152.

Effects of Addition of Insulin-Transferrin-Sodium Selenite on Effect of Production, Hypothermic Storage and Routine Freezing of Bovine in vitro Embryos

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Mingguo, LI Qingchun, CHEN Xin, LU Shihao, HE Fan, QI Mengfan, ZHANG Huapeng, REN Yujun, ZHANG Qingze, FU Binbin, XU Mengsi, AI Zikai, YAN Kun, FENG Yun, HUA Zaidong, HUANG Tao, BI Yanzhen. Effects of TET Demethylase on Porcine Oocyte Development [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1907-1917.
[2]	JI Wenhui, WANG Yuling, HE Honghong, FU Wei, LAN Daoliang. Effects of Vitamin A on the Maturation and Subsequent Development of Yak Oocytes in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4707-4714.
[3]	LI Shujing, LI Xin, FENG Chuntao, GAO Weijing, SUN Guilai, GU Sukun, YU Wenli. Effect of N-carbamylglutamate on Superovulation and Blood Indexes in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3699-3707.
[4]	HAO Tong, WANG Jingjing, HAO Haisheng, DU Weihua, PANG Yunwei, ZHAO Shanjiang, ZOU Huiying, ZHANG Peipei, ZHU Huabin, ZHAO Xueming. Effect of Vitrification on Epigenetics of Mammalian MⅡ Oocytes [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2866-2875.
[5]	ALAFAT·Amulati, ZHAO Xi, NURIBIYAMU·Maimaitituoheti, AERMAN·Haire, LIU GuoShi, ABULIZI·Wusiman. Effects of Astaxanthin on Mouse Implantation Embryos Development and Gene Expression [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 148-155.
[6]	FU Yanfeng, FANG Xiaomin, LI Bixia, ZHAO Weimin, WANG Xuemin, CHEN Zhe, REN Shouwen. A Review of Some Factors Related to Embryo Implantation in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2676-2682.
[7]	ZHAO Yuhan, YAO Xuerui, JIANG Wenjie, WANG Huaqing, XU Yongnan, GAO Qingshan. Effect of Adding Ge-132 on Bovine Embryo Development Potential in vitro [J]. , 2018, 45(9): 2537-2543.
[8]	SHI Xian, XIONG Xian-rong, LAN Dao-liang, HU Jia-jia, CAI Wen-yi, ZHANG Da-wei, LI Jian. Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes [J]. , 2017, 44(1): 155-160.
[9]	SUN Hong-liang, XU Mei-hua, WANG Yao, LU Wen-geng. Research Progress on the Function of Wnt Signaling Pathway in the Development of Bovine Placenta [J]. , 2017, 44(1): 161-166.
[10]	REN Hong-yan, Xiao Hong-wei, LI Xiao-min, XU Zhen-yu, BI Yan-zhen, HUA Zai-dong, ZHENG Xin-min. Establishment and Application of Nested PCR Method for Detecting Mutation in Single Cell [J]. , 2016, 43(10): 2566-2571.
[11]	LU Xing-rong, LI Zhi-Peng, SUN Jun-ming, LV Ling-yan, XU Zhong-feng, LIU Qing-you, SHI De-shun, CUI Kui-qing. Effect of DNA Methylation Inhibitor 5-Aza-CdR on Patterns of Pluripotent-related Genes and Methylation Genes in Handmade Cloning Embryo [J]. , 2016, 43(9): 2240-2248.
[12]	LIU Xiao-lin, DU Feng-jiao, LEI Chuan, RUAN Qiu-yan, WU Zhu-lian, SUN Hong-liang, ZHAO Xin, DENG Yan-fei, LU Feng-hua, SHI De-shun. Effects of Low Oxygen Tension for in vitro Oocyte Maturation and Early Embryo Development through Hypoxia-inducible Factor (HIF) [J]. , 2015, 42(6): 1559-1565.
[13]	CEN Wen, HUA Zai-dong, ZHENG Xin-min, XIAO Hong-wei, ZHANG Li-ping, WU Min-yao. Effect of COCs on in vitro Maturation and Parthenogenetic Development of Pig Oocyte [J]. , 2015, 42(3): 638-643.
[14]	CAO Xin-yan, LIU Han-lu, ZHAO Jing-bo, JING Yi, YUE Zhi-gang, LI Guang-yu. Influencing Factors and Regulation of Embryonic Diapause in Mink [J]. , 2013, 40(3): 169-172.
[15]	PEI Yan;FENG Chun-tao;ZHOU Yan-hua;ZHU Shi-en;YU Wen-li;. Study and Application on Beef Cattle Embryo Bisection [J]. , 2011, 38(11): 147-150.