添加Ge-132对牛体外胚胎发育潜能的影响

doi:10.16431/j.cnki.1671-7236.2018.09.025

Abstract

Abstract:

This study was aimed to investigate the effect of carboxyethylgermanium sesquioxide (Ge-132) on the development rate of embryos,the number of embryonic cells,the early embryonic reactive oxygen species (ROS) level and the related apoptotic genes in the embryo after activation of bovine parthenogenesis.The effects of different concentrations of Ge-132 (0,10,100 and 200 μg/mL) on the embryo development of parthenogenetically activated bovine embryos were studied,Hoechst staining was performed on the 8th day embryos,the number of cells was counted under the microscope.The ROS level was detected in the early embryos by DCFH-DA staining,and the data were analyzed statistically after the measurement of fluorescence intensity by Image J.In addition,the apoptosis related genes (Caspase-3,Bax,Bcl-xl and Survivin) in the embryo were analyzed by RT-PCR method.The results showed that there was no significant difference in the embryo rate between 10 μg/mL Ge-132 treatment and control groups (P>0.05),but 10 μg/mL Ge-132 could reduce the ROS level in the one cell stage.The number of total cells increased significantly in 10 μg/mL Ge-132 treatment group than that of control group (P<0.05).Furthermore,by detecting the mRNA transcriptional level of apoptosis related genes,it was found that compared with control group,the expression level of the apoptotic gene Caspase-3 was significantly decreased in 10 μg/mL Ge-132 treatment group (P<0.05),whereas the expression level of anti-apoptotic gene Survivin was significantly increased in 10 μg/mL Ge-132 treatment group (P<0.05).In conclusion,the supplementation of 10 μg/mL Ge-132 to the early embryo culture medium could reduce the intracellular ROS level and the apoptosis induced by oxidative stress in the embryo,thereby improving the developmental potential of the bovine embryos after parthenogenetic activation.

Key words: bovine; embryo; Ge-132; ROS; apoptosis

CLC Number:

S814.6

ZHAO Yuhan, YAO Xuerui, JIANG Wenjie, WANG Huaqing, XU Yongnan, GAO Qingshan. Effect of Adding Ge-132 on Bovine Embryo Development Potential in vitro[J]. , 2018, 45(9): 2537-2543.

References

[1] 庄利利,胡德宝,方南洙,等.哺乳动物胚胎的活性氧来源及其抗氧化机制[J].中国畜牧兽医,2013,40(3):173-176.ZHUANG L L,HU D B,FANG N Z,et al.The source of active oxygen in mammalian embryos and its antioxidant mechanism[J].China Animal Husbandry & Veterinary Medicine,2013,40(3):173-176.(in Chinese)
[2] HOSTETLER C E,KINCAID R L,MIRANDO M A.The role of essential trace elements in embryonic and fetal development in livestock[J]. Veterinary Journal,2003,166(2):125-139.
[3] TAKAHASHI M.Oxidative stress and redox regulation on in vitro development of mammalian embryos[J].The Journal of Reproduction and Development,2012,58(1):1-9.
[4] 高婷,王子旭,陈耀星,等.ROS介导的氧化应激与自噬[J].中国畜牧兽医,2018,45(3):656-662.GAO T,WANG Z X,CHEN Y X,et al.Oxidative stree and autophagy mediated by reactive oxygen species[J].China Animal Husbandry & Veterinary Medicine,2018,45(3):656-662.(in Chinese)
[5] CORDOVA B,MORATO R,TZQUIERDO D,et al.Effect of the addition of insulin-transferrin-selenium and/or L-ascorbic acid to the in vitro maturation of prepubertal bovine oocytes on cytoplasmic maturation and embryo development[J].Theriogenology,2010,74(8):1341-1348.
[6] KAPLAN B J,PARISH W W,ANDRU G M,et al.Germane facts about germanium sesquioxide:Ⅰ.Chemistry and anticancer properties[J]. Journal of Alternative & Complementary Medicine,2004,10(2):337-344.
[7] BRUTKIEWICZ R R,SUZUKI F.Biological activities and antitumor mechanism of an immunopotentiating organogermanium compound,Ge-132(review)[J].In Vivo,1987,1(4):189-203.
[8] PRONAI L,ARIMORI S.Decreased plasma superoxide scavenging activity in immunological disorders-carboxyethylgermanium sesquioxide (Ge-132) as a promoter of prednisolone[J].Biotherapy,1992,4(1):1-8.
[9] YANG M K,KIM Y G.Protective role of germanium-132 against paraquat-induced oxidative stress in the livers of senescence-accelerated mice[J].Journal of Toxicology Environmental Health,1999,58(5):289-297.
[10] PI J,ZENG J,LUO J J,et al.Synthesis and biological evaluation of germanium(IV)-polyphenol complexes as potential anti-cancer agents[J]. Bioorganic & Medicinal Chemistry Letters,2013,23(10):2902-2908.
[11] HAO Y,LAI L,MAO J,et al.Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer[J].Biology Reproduction,2003,69(2):501-507.
[12] KIM E,HWANG S U,YOON J D,et al.Carboxyethylgermanium sesquioxide (Ge-132) treatment during in vitro culture protects fertilized porcine embryos against oxidative stress induced apoptosis[J]. The Journal Reproduction Development,2017,63(6):581-590.
[13] JIANG J,YAO S,CAI H H,et al.Synthesis and synergetic effects of chrysin-organogermanium (IV) complex as potential anti-oxidant[J].Bioorganic & Medicinal Chemistry Letters,2013,23(20):5727-5732.
[14] WADA T,HANYU T,NOZAKI K,et al.Antioxidant activity of Ge-132,a synthetic organic germanium,on cultured mammalian cells[J]. Biological & Pharmaceutical Bulletin,2018,41(5):749-753.
[15] YAO S,JIANG J,YANG P,et al.Synthesis,characterization and antioxidant activity of a novel organo-germanium sesquioxide with resveratrol[J].Bulletin-Korean Chemical Society,2012,33(4):1121-1122.
[16] KIM E,JEON Y,KIM D Y,et al.Antioxidative effect of carboxyethylgermanium sesquioxide (Ge-132) on IVM of porcine oocytes and subsequent embryonic development after parthenogenetic activation and IVF[J].Theriogenology,2015,84(2):226-236.
[17] ZAKERI Z,BURSCH W,TENNISWOOD M,et al.Cell death:Programmed,apoptosis,necrosis,or other[J].Cell Death & Differentiation,1995,2(2):87-96.
[18] TSUJⅡ H,LEE J H,HOSSAIN M S,et al.The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes[J].Reproductive Medicine & Biology,2008,8(1):19-24.
[19] ELMORE S.Apoptosis:A review of programmed cell death[J].Toxicologic Pathology,2007,35(4):495-516.
[20] CHEONG S A,KIM E,KWAK S S,et al.Improvement in the blastocyst quality and efficiency of putative embryonic stem cell line derivation from porcine embryos produced in vitro using a novel culturing system[J].Molecular Medicine Reports,2015,12(2):2140-2148.
[21] 金玲,何小进,尹玲桃,等.神经节苷脂对脑瘫大鼠大脑皮质区Nestin、NGF表达的影响[J].湖南中医药大学学报,2014,34(8):13-16.JIN L,HE X J,YIN L T,et al.Sdudy ganglioside on expression of Nestin and NGF in cerebral palsy cerebral cortex[J].Journal of Hunan University of Chinese Medicine,2014,34(8):13-16.(in Chinese)
[22] WHENTLEY S P,MCHEISH I A.Survivin:A protein with dual roles in mitosis and apoptosis[J].International Review of Cytology-A Survey of Cell Biology,2005,247:35-88.

Effect of Adding Ge-132 on Bovine Embryo Development Potential in vitro

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