卵丘细胞对猪卵母细胞体外成熟及孤雌胚胎体外发育的影响

doi:10.11843/j.issn.1671-7236.2015.03.020

Abstract

Abstract: This experiment was conducted to study the effect of the concentration of epidermal growth factor (EGF) and times of oocytes got rid of cumulus cells on in vitro maturation and development of parthenogenetic embryoes of pig oocytes.The embryos were treated with different concentrations (0,10,15,20,30,40 ng/mL)of EGF for 44 h,maturation rate,cleavage rate and blastocyst rate of oocytes were compared.Removing cumulus cell in different time (18,24,38,44 h) after cultivation to study effect of the time of removing the cumulus cell on maturation rate and the developmental capacity of parthenogenetic embryos.The results showed that the cleavage rate and blastocyst rate of oocytes in maturation media with 10 ng/mL EGF were significantly higher than blank treatment groups (P <0.05).There were no significant differences in the maturation rates of removing cumulus cell after cultivation of 18 h and cultivation of 44 h (P >0.05);The cleavage rate and blastocyst rate of oocytes of removing cumulus cell after cultivation of 18 h were significantly higher than cultivation of 44 h in the together culture group (P <0.05);There were no significant differences in the cleavage rate of removing cumulus cell after cultivation of 18 h and cultivation of 44 h (P >0.05),but the blastocyst rate of oocytes of removing cumulus cell after cultivation of 18 h were significantly higher than cultivation of 44 h in the alone culture group (P <0.05).These results indicated that the treatment of 10 ng/mL EGF was better for porcine oocyte culture in vitro and parthenogeretic development.Removing cumulus cell after cultivation of 18 h could improve the development of parthenogenetic embryos.

Key words: porcine; EGF; cumulus cells; in vitro maturation; embryo development

CLC Number:

S814.6

CEN Wen, HUA Zai-dong, ZHENG Xin-min, XIAO Hong-wei, ZHANG Li-ping, WU Min-yao. Effect of COCs on in vitro Maturation and Parthenogenetic Development of Pig Oocyte[J]. , 2015, 42(3): 638-643.

References

[1] Lai L,Prather R S.Creating genetieally modified pigs by using nuelear transfer[J].Reprod Biol Elldoerino,2003,1:82.

[2] Coy P,Romar R.In vitro production of pig embryos:A point of view[J].Reprod Fertil Dev,2002,14(5-6):275-286.

[3] Day B N.Reproductive biotechnologies:Current status in porcine reproduction[J].Animal Reproduction Science,2000,60:161-172.

[4] 刘晓辉,郎洪彦,金海国,等.猪卵母细胞体外成熟的研究进展[J].黑龙江动物繁殖,2011,19(4):7-11.

[5] Procházka R,Petlach M,Nagyova E,et al.Effect of epidermal growth factor-like peptides on pig cumulus cell expansion,oocyte maturation,and acquisition of developmental competence in vitro:Comparison with gonadotropins[J].Society for Reproduction and Fertility,2011,141:425-435.

[6] Abeydeera L R,Wang W H,Cantley T C,et al.Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor[J].Theriogenology,2000,54:787-797.

[7] Sutton M L,Gilchrist R B,Thompson J G.Effects of in vivo and in vitro environment on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity[J].Human Reproduction Update,2003,9(1):35-48.

[8] 石俊松,罗绿花,周荣,等.卵丘细胞对卵母细胞成熟受精和胚胎发育的影响试验[J].广东畜牧兽医科技,2014,39(1):32-35.

[9] Park K W.Exposure of bovine oocyte to EGF during maturation allows them to develop to blastcysts in a chemically defined medium[J].Theriogenology,1997,48:1127-1135.

[10] 马会明,黑常春,张永芳,等.EGF通过PI3K/AKT通路介导小鼠卵巢颗粒细胞增殖[J].宁夏医科大学学报,2014,36(5):483-490.

[11] Ben-Ami I,Komsky A,Bern O,et al.In vitro maturation of human germinal vesicle-stage oocytes:Role of epidermal growth factor-like growth factors in the culture medium[J].Hum Reprod,2011,26(1):76-81.

[12] 王光演,牛志宏,冯云.表皮生长因子样因子在LH峰效应中的作用及临床研究进展[J].生殖与避孕,2014,34(6):497-500.

[13] 姜李乐,韦多,张翠莲.人未成熟卵母细胞培养体系研究进展[J].生殖与避孕,2014,34(5):388-394.

[14] 兰宗宝,何若钢,莫品方,等.猪卵母细胞第一极体排出与否对其早期胚胎发育的影响[J].西南师范大学学报(自然科学版),2008,33(5):64-68.

[15] Geshi M,Takenouchi N,Yamauchi N,et al.Effects of sodium pyruvate in nonserum maturation medium on maturation,fertilization,and subsequent development of bovine oocytes with or without cumulus cells[J].Biol Reprod,2000,63:1130-1134.

[16] 朱桂玉,冯书堂,李吉涛,等.猪卵丘卵母细胞复合体和裸卵中卵母细胞发育及基因表达差异的研究[J].中国农业科学,2008,1:208-214.

[17] Atikuzzaman M,Koo O J,Kang J T,et al.The 9-cis retinoic acid signaling pathway and its regulation of prostaglandinendoperoxide synthase 2 duringin vitro maturation of pig cumulus cell-oocyte complexes and effects on parthenogenetic embryo production [J].Biology of Reporduction,2011,84:1272-1281.

[18] Dieci C,Lodde V,Franciosi F,et al.The effect of cilostamide on gap junction communication dynamics,chromatin remodeling,and competence acquisition in pig oocytes following parthenogenetic activation and nuclear transfer[J].Biol Reprod September,2013,89(3):68.

[19] 曾申明,朱士恩,余文莉,等.卵泡直径和卵丘细胞对牛卵母细胞体外受精后发育潜力的影响[J].农业生物技术学报,2000,4:357-360.

Effect of COCs on in vitro Maturation and Parthenogenetic Development of Pig Oocyte

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