Abstract: Bovine coccidiosis is a highly pathogenic parasitic protozoan disease caused by one or more species of Eimeria, with a wide range of epidemiological scope, which can lead to malabsorption of nutrients, developmental delays, watery or bloody diarrhoea and other symptoms, and even cause death, which seriously harms the development of the cattle industry.Currently, bovine coccidiosis is mainly prevented and controlled through drug prevention, but with the emergence of drug-resistant strains of worms and the concern about veterinary drug residues, the research and development of new anticoccidial drugs is urgent.As an alternative to experimental animal research, in vitro culture can artificially simulate the in vivo environment and conditions of the natural host of bovine coccidia at relatively low cost, which is of great significance for the study of the invasion mechanism of coccidia, the interaction between coccidia and the host, as well as the evaluation of the efficacy of anti-coccidial drugs.In the established in vitro culture system, bovine coccidia can complete part or all of the endogenous development process from ascospores to oocysts, which opens up new ways to study the life cycle stages of the worm and new control strategies.By reviewing the relevant data on in vitro culture of bovine coccidia at home and abroad, the author elaborated on the isolation and purification of coccidian ascospores, primary cell and cell line culture, in vitro culture conditions and influencing factors, and the application aspects of in vitro culture, with a view to providing a reference for the research and development of a safe and effective vaccine and preventive drug for bovine coccidia.

Key words: bovine coccidiosis; Eimeria; in vitro culture; pharmacodynamic evaluation