鸡球虫体外培养模型及应用研究进展

doi:10.16431/j.cnki.1671-7236.2024.01.029

Abstract

Abstract: Coccidiosis is a parasitic protozoan disease caused by one or more species of Eimeria parasitizing in the intestinal epithelial cells of chickens.The disease quickly leads to weight loss, malnutrition, intestinal damage, etc., and has a high economic impact on the poultry industry worldwide.The life cycle of coccidia includes a switch from asexual to sexual stages and is often restricted to a single host.Current research mainly focused on cell biology, protein expression and transport mechanisms in different life stages, host cell infection, host-parasite interactions, new anticoccidial drug screens, etc.Given the diversity of research questions and the requirement of reduce and replace animal experiments, establishing efficient in vitro culture models has become essential for coccidia biological characteristics, anti-coccidia vaccines and drugs study.In this review, the author summarized the in vitro culture model of the poultry parasite coccidia and its application in drug screening, including the chicken embryo culture model, two-dimensional culture models (primary cell culture model, immortalized cell line culture model) and 3D organoid culture model.Breakthroughs in culture techniques have opened new avenues for research on the life cycle stages of coccidia and new intervention strategies and also provide a reference for in-depth understanding of the pathogenic mechanism of coccidia and the development of the anti-coccidia drugs.

Key words: chicken; Eimeria; in vitro culture model

CLC Number:

S852.72

PAN Xiaoting, LI Juan, CHEN Bing, LIU Junjie, QI Nanshan, LIAO Shenquan, LYU Minna, LIN Xuhui, CAI Haiming, HU Junjing, SONG Yongle, BAI Yinshan, SUN Mingfei. Research Progress on Chicken Coccidia in vitro Culture Models and Application[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 292-301.

References

[1] 刘颖, 严斯刚, 汪嘉彦, 等.鸡艾美耳球虫病病原调查及优势虫种鉴定[J].湖南畜牧兽医, 2018, 2:44-46. LIU Y, YAN S G, WANG J Y, et al.Pathogen investigation and identification of dominant species of eimeriasis in chickens[J].Hunan Journal of Animal Science & Veterinary Medicine, 2018, 2:44-46.(in Chinese)
[2] 陈自林.鸡球虫病预防和治疗措施[J].中国畜禽种业, 2019, 15(2):185. CHEN Z L.Prevention and treatment measures for chicken coccidiosis[J].The Chinese Livestock and Poultry Breeding, 2019, 15(2):185.(in Chinese)
[3] WONDIMU A, MESFIN E, BAYU Y.Prevalence of poultry coccidiosis and associated risk factors in intensive farming system of Gondar town, Ethiopia[J].Veterinary Medicine International, 2019, 2019:5748690.
[4] EL-SHALL N A, ABD E M, ALBAQAMI N M, et al.Phytochemical control of poultry coccidiosis:A review[J].Poultry Science, 2022, 101(1):101542.
[5] ACOSTA D J, HERNANDEZ D L R A.An overview of peripheral blood mononuclear cells as a model for immunological research of Toxoplasma gondii and other apicomplexan parasites[J].Frontiers in Cellular and Infection Microbiology, 2019, 9:24.
[6] KIRSCHNER K M.Reduce, replace, refine-Animal experiments[J].Acta Physiologica (Oxford, England), 2021, 233(3):e13726.
[7] 王婧, 杨海峰, 王妲妲, 等.鸡胚及禽类细胞系在生物医药领域的应用及研究进展[J].中国畜牧兽医, 2020, 47(11):3783-3791. WANG J, YANG H F, WANG D D, et al.The application and research progress of chicken embryo and avian cell lines in biomedicin[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(11):3783-3791.(in Chinese)
[8] LONG P L.Development of Eimeria tenella in avian embryos[J]. Nature, 1965, 208(5009):509-510.
[9] LONG P L.The growth of some species of Eimeria in avian embryos[J].Parasitology, 1966, 56(3):575-581.
[10] KOGUT M H, GORE T C, LONG P L.Serial passage of Eimeria tenella and E.necatrix in turkey embryos[J].Parasitology, 1983, 86(Pt 2):199-209.
[11] 吕文顺, 赵开元, 陈震环, 等.脆弱艾美尔球虫在鸡胚培养中内生性发育史的研究[J].畜牧兽医学报, 1985, 1:35-40. LYU W S, ZHAO K Y, CHEN Z H, et al.Study of endogenous cycle of an avain coccidium Eimeria tenella in chicken embryos[J].Chinese Journal of Animal and Veterinary Sciences, 1985, 1:35-40.(in Chinese)
[12] 谢明权, 温列娜, 吴惠贤, 等.鸡球虫Eimeria tenella体外繁殖适应株的建立研究[J].广东畜牧兽医科技, 2000, 4:12-15. XIE M Q, WEN L N, WU H X, et al.Establishment of in vitro propagation adaptations for Eimeria tenella in chicken coccidia[J].Guangdong Journal of Animal and Veterinary Science, 2000, 4:12-15.(in Chinese)
[13] HAFEEZ M A, AKHTAR M, AYAZ M M.Some observations on the adaptation of Eimeria tenella (local isolates) sporozoites on chicken embryos through chorioallantoic membrane[J].Journal of Veterinary Science, 2006, 7(1):59-61.
[14] JIANG L, ZHAO Q, ZHU S, et al.Establishment of Eimeria tenella (local isolate) in chicken embryos[J].Parasite, 2012, 19(3):285-289.
[15] ALNASSAN A A, SHEHATA A A, KOTSCH M, et al.Embryonated chicken eggs as an alternative model for mixed Clostridium perfringens and Eimeria tenella infection in chickens[J].Parasitology Research, 2013, 112(6):2299-2306.
[16] CONWAY D P, GUYONNET V, MICHENER S, et al.Efficacy of semduramicin and salinomycin against Eimeria maxima in a laboratory test using two levels of oocyst inocula[J].Poultry Science, 1995, 74(12):1942-1947.
[17] LONG P L, MILLARD B J.Eimeria infection of chicken embryos:The effect of known anticoccidial drugs against E.tenella and E.mivati[J].Avian Pathology, 1973, 2(2):111-125.
[18] JEFFERS T K, WAGENBACH G E.Embryonic response to Eimeria tenella infection[J].The Journal of Parasitology, 1970, 56(4):656-662.
[19] WATKINS K L, BROOKS M A, JEFFERS T K, et al.The effect of in ovo oocyst or sporocyst inoculation on response to subsequent coccidial challenge[J].Poultry Science, 1995, 74(10):1597-1602.
[20] WEBER F H, GENTEMAN K C, LEMAY M A, et al.Immunization of broiler chicks by in ovo injection of infective stages of Eimeria[J].Poultry Science, 2004, 83(3):392-399.
[21] MARUGAN-HERNANDEZ V, JEREMIAH G, AGUIAR-MARTINS K, et al.The growth of Eimeria tenella:Characterization and application of quantitative methods to assess sporozoite invasion and endogenous development in cell culture[J]. Frontiers in Cellular and Infection Microbiology, 2020, 10:579833.
[22] 古少鹏, 侯金环, 陈赵英, 等.柔嫩艾美耳球虫在鸡胚盲肠上皮细胞中体外培养营养条件的研究[J].畜牧兽医学报, 2010, 41(12):1622-1628. GU S P, HOU J H, CHEN Z Y, et al.Study on nutrition condition of E.tenella cultivation in cecum epithelial cells from chicken embryo in vitro[J].Chinese Journal of Animal and Veterinary Sciences, 2010, 41(12):1622-1628.(in Chinese)
[23] DORAN D J, AUGUSTINE P C.Comparative development of Eimeria tenella from sporozoites to oocysts in primary kidney cell cultures from gallinaceous birds[J].The Journal of Protozoology, 1973, 20(5):658-661.
[24] DORAN D J.Comparative development of Eimeria tenella in primary cultures of kidney cells from the chicken, pheasant, partridge, and turkey[J].The Journal of Protozoology, 1971, 57(6):1376-1377.
[25] TIERNEY J, MULCAHY G.Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro[J].Parasitology Research, 2003, 90(4):301-304.
[26] 姜连连, 林矫矫, 韩红玉, 等.柔嫩艾美耳球虫DF-1细胞培养体系的建立及应用[J].中国兽医科学, 2011, 41(6):551-556. JIANG L L, LIN J J, HAN H Y, et al.Establishment and application of DF-1 cell culture system for the sporozoites of Eimeria tenella[J].Chinese Veterinary Science, 2011, 41(6):551-556.(in Chinese)
[27] NACIRI-BONTEMPS M.Reproduction of the cycle of coccidia Eimeria acervulina (Tyzzer, 1929) in cell cultures of chicken kidneys[J].Annales de Recherches Veterinaires.Annals of Veterinary Research, 1976, 7(3):223-230.
[28] 蒋金书, 胡景辉.柔嫩艾美耳球虫和堆型艾美耳球虫在鸡胚培养和鸡肾细胞中的发育[J].中国农业大学学报, 1998, S2:162-163. JIANG J S, HU J H.Development of E.tenella and E.acervulina in chicken embryo culture and chicken kidney cells[J].Journal of China Agricultural University, 1998, S2:162-163.(in Chinese)
[29] 秦建华, 赵月兰, 张明勇, 等.堆型艾美耳球虫早熟减毒株裂殖子的细胞培养[J].中国兽医寄生虫病, 2000, 1:22-23. QIN J H, ZHAO Y L, ZHANG M Y, et al.Cell culture of the early maturing attenuated strain merozoites of E.acervulina[J].Chinese Journal of Veterinary Psrasitology, 2000, 1:22-23.(in Chinese)
[30] 徐镔蕊, 佘锐萍, 汪明, 等.巨型艾美耳球虫Eimeria maxima内生发育阶段的细胞化学研究Ⅱ.非特异性酯酶、碱性磷酸酶、酸性磷酸酶[J].中国兽医杂志, 1994, 20(7):12. XU B R, SHE R P, WANG M, et al.Cytochemical study of endogenous developmental stage of Eimeria maxima macrocellus Ⅱ.Nonspecific esterases, alkaline phosphatase, acid phosphatase[J].Chinese Journal of Veterinary Medicine, 1994, 20(7):12.(in Chinese)
[31] 石梦云, 王黎霞, 赵小涵, 等.巨型艾美耳球虫在4种细胞系中培养的比较[J].中国兽医杂志, 2023, 59(4):41-46. SHI M Y, WANG L X, ZHAO X H, et al.Comparison of Eimeria maxima culture in four cell lines[J].Chinese Journal of Veterinary Medicine, 2023, 59(4):41-46.(in Chinese)
[32] DORAN D J.Survival and development of five species of chicken coccidia in primary chicken kidney cell cultures[J].The Journal of Parasitology, 1971, 57(5):1135-1137.
[33] 陈刚, 蒋金书.布氏艾美耳球虫和巨型艾美耳球虫在鸡肾细胞及鸡胚培养中的发育[J].中国农业大学学报, 1998, S2:164. CHEN G, JIANG J S.Development of E.brunetti and E.maxima in chicken kidney cells and embryo cultures[J].Journal of China Agricultural University, 1998, S2:164.(in Chinese)
[34] PATTON W H.Eimeria tenella:Cultivation of the asexual stages in cultured animal cells[J].Science, 1965, 150(3697):767-769.
[35] XU Z, ZHENG M, ZHANG L, et al.Effect of ATP and Bax on the apoptosis of Eimeria tenella host cells[J].BMC Veterinary Research, 2017, 13(1):399.
[36] HIOB L, KOETHE M, SCHARES G, et al.Experimental Toxoplasma gondii and Eimeria tenella co-infection in chickens[J].Parasitology Research, 2017, 116(11):3189-3203.
[37] RAJ G D, AARTH S, SELVABHARATHI R, et al.Real-time PCR-based quantification of Eimeria genomes:A method to outweigh underestimation of genome numbers due to PCR inhibition[J].Avian Pathology, 2013, 42(4):304-308.
[38] PACHECO N D, VETTERLING J M, DORAN D J.Ultrastructure of cytoplasmic and nuclear changes in Eimeria tenella during first-generation schizogony in cell culture[J].The Journal of Parasitology, 1975, 61(1):31-42.
[39] SHI T Y, LLU X Y, HAO L L, et al.Transfected Eimeria tenella could complete its endogenous development in vitro[J].The Journal of Parasitology, 2008, 94(4):978-980.
[40] YAN X, HAN W, LLU X, et al.Exogenous nitric oxide stimulates early egress of Eimeria tenella sporozoites from primary chicken kidney cells in vitro[J].Parasite, 2021, 28:11.
[41] 谢明权, 谢宏料, 吴惠贤, 等.细胞培养筛选抗球虫剂程序的研究[J].畜牧兽医学报, 1997, 3:87-92. XIE M Q, XIE H L, WU H X, et al.Standard procedure on screening screening anticoccidials in vitro[J].Chinese Journal of Animal and Veterinary Sciences, 1997, 3:87-92.(in Chinese)
[42] DEL CACHO E, GALLEGO M, FRANCESCH M, et al.Effect of artemisinin on oocyst wall formation and sporulation during Eimeria tenella infection[J].Parasitology International, 2010, 59(4):506-511.
[43] RENTERIA-SOLIS Z, ZHANG R, TAHA S, et al.A modified method for purification of Eimeria tenella sporozoites[J].Parasitology Research, 2020, 119(4):1429-1432.
[44] DEBBOU-IOUKNANE N, NERIN C, AMRANE M, et al.In vitro anticoccidial activity of olive pulp (Olea europaea L.var.Chemlal) extract against Eimeria oocysts in broiler chickens[J].Acta Parasitologica, 2019, 64(4):887-897.
[45] BUSSIōRE F I, NIEPCERON A, SAUSSET A, et al.Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development[J].Parasites & Vectors, 2018, 11(1):44.
[46] TAHA S, NGUYEN-HO-BAO T, DAUGSCHIES A, et al.In vitro infection of Madin-Darby bovine kidney (MDBK) cells with Eimeria acervulina sporozoites:Quantitative analysis of parasite cellular invasion and replication using Real-time polymerase chain reaction (PCR)[J].Parasitology Research, 2021, 120(7):2689-2693.
[47] GADELHAQ S M, ARAFA W M, ABOLHADID S M.In vitro activity of natural and chemical products on sporulation of Eimeria species oocysts of chickens[J].Veterinary Parasitology, 2018, 251:12-16.
[48] DEL C E, GALLEGO M, LOPEZ-BERNAD F, et al.Differences in Hsp70 expression in the sporozoites of the original strain and precocious lines of Eimeria tenella[J].Journal of Parasitology, 2005, 91(5):1127-1131.
[49] 廖义潇, 万涛, 李登富, 等.牛至油-青蒿素复方纳米乳制备及治疗鸡球虫病效果评价[J].中兽医医药杂志, 2021, 40(4):28-33. LIAO Y X, WAN T, LI D F, et al Preparation of oregano essential oil-arteminisin nanoemulsion and its therapeutic efficacies on chicken coccidiosis[J].Journal of Traditional Chinese Veterinary Medicine, 2021, 40(4):28-33.(in Chinese)
[50] KHALAFALLA R E, MULLER U, SHAHIDUZZAMAN M, et al.Effects of curcumin (diferuloylmethane) on Eimeria tenella sporozoites in vitro[J].Parasitology Research, 2011, 108(4):879-886.
[51] CHANG L Y, DI K Q, XU J, et al.Effect of natural garlic essential oil on chickens with artificially infected Eimeria tenella[J]. Veterinary Parasitology, 2021, 300:109614.
[52] FELICI M, TUGNOLI B, PIVA A, et al.In vitro assessment of anticoccidials:Methods and molecules[J].Animals (Basel), 2021, 11(7):1962.
[53] 江杨帆, 何素冰, 吴纳新, 等.细胞三维培养与传统细胞培养的应用现状[J].安徽医学, 2018, 39(5):625-627. JIANG Y F, HE S B, WU N X, et al.Application status of 3D cell culture and traditional cell culture[J].Anhui Medical Journal, 2018, 39(5):625-627.(in Chinese)
[54] 李娟娟, 张君涛, 赵英博, 等.类器官的研究进展及应用前景[J].中国畜牧兽医, 2021, 48(6):1985-1994. LI J J, ZHANG J T, ZHAO Y B, et al.Research progress and application prospect of organoids[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(6):1985-1994.(in Chinese)
[55] 马连素, 李军男.浅析类器官技术的发展现状[J].实验动物科学, 2023, 40(2):94-100. MA L S, LI J N.Analysis on the development status of organoid technology[J].Laboratory Animal Science, 2023, 40(2):94-100.(in Chinese)
[56] 王曼茜, 李庆豪, 孙娟, 等.类器官的培养方法及应用前景[J].中国畜牧兽医, 2023, 50(7):2688-2696. WANG M Q, LI Q H, SUN J, et al.Culture methods and application prospect of organoids[J].China Animal Husbandry & Veterinary Medicine, 2023, 50(7):2688-2696.(in Chinese)
[57] NASH T J, MORRIS K M, MABBOTT N A, et al.Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions[J].Communications Biology, 2021, 4(1):377.
[58] NIKOLAEV M, MITROFANOVA O, BROGUIERE N, et al.Homeostatic mini-intestines through scaffold-guided organoid morphogenesis[J].Nature, 2020, 585(7826):574-578.
[59] BAYDOUN M, VANNESTE S B, CREUSY C, et al.Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis:Proof of concept[J].Scientific Reports, 2017, 7(1):17288.
[60] HEO I, DUTTA D, SCHAEFER D A, et al.Modelling Cryptosporidium infection in human small intestinal and lung organoids[J].Nature Microbiology, 2018, 3(7):814-823.
[61] DELGADO BETANCOURT E, HAMID B, FABIAN B T, et al.From entry to early dissemination-Toxoplasma gondii's initial encounter with its host[J].Frontiers in Cellular and Infection Microbiology, 2019, 9:46.
[62] SEO H H, HAN H W, LEE S E, et al.Modelling Toxoplasma gondii infection in human cerebral organoids[J].Emerging Microbes & Infections, 2020, 9(1):1943-1954.
[63] MARTORELLI DI GENOVA B, WILSON S K, DUBEY J P, et al.Intestinal delta-6-desaturase activity determines host range for Toxoplasma sexual reproduction[J]. PLoS Biology, 2019, 17(8):e3000364.
[64] LUU L, JOHNSTON L J, DERRICOTT H, et al.An open-format enteroid culture system for interrogation of interactions between Toxoplasma gondii and the intestinal Epithelium[J]. Frontiers in Cellular and Infection Microbiology, 2019, 9:300.
[65] JANNASCH M, GROEBER F, BRATTIG N W, et al.Development and application of three-dimensional skin equivalents for the investigation of percutaneous worm invasion[J].Experimental Parasitology, 2015, 150:22-30.
[66] AMIRIFAR L, SHAMLOO A, NASIRI R, et al.Brain-on-a-chip:Recent advances in design and techniques for microfluidic models of the brain in health and disease[J]. Biomaterials, 2022, 285:121531.
[67] KASENDRA M, TOVAGLIERI A, SONTHEIMER-PHELPS A, et al.Development of a primary human small intestine-on-a-chip using biopsy-derived organoids[J].Scientific Reports, 2018, 8(1):2871.
[68] MANAFI N, SHOKRI F, ACHBERGER K, et al.Organoids and organ chips in ophthalmology[J].The Ocular Surface, 2021, 19:1-15.
[69] RAMIREZ-FLORES C J, TIBABUZO P A, GALLEGO-LOPEZ G M, et al.Transcending dimensions in apicomplexan research:From two-dimensional to three-dimensional in vitro cultures[J].Microbiology and Molecular Biology Reviews, 2022, 86(2):e2522.

Research Progress on Chicken Coccidia in vitro Culture Models and Application

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