烈性噬菌体KM104对不同血清型鸡源沙门菌的防治效果评价

doi:10.16431/j.cnki.1671-7236.2024.01.028

Abstract

Abstract: 【Objective】 This study was to investigate the preventive and therapeutic effects of lytic phage KM104 strain on Salmonella spp.infection in chickens with different serotypes.【Method】 In this experiment the in vitro lysis spectrum and in vivo safety of KM104 strain and the attack dose and the multiplicity of infection were determined.According to the time, frequency and concentration for use of the bacteriophage KM104 strains, the prevention and therapeutic models of Salmonella spp.infection from chickens with three different serotypes in White feather laying hens were constructed.By recording the survival number and survival rate, growth performance, clinical symptoms and pathological changes of test animals and measuring bacterial excretion of anal swab, bacterial load of spleen and organ index, the prevention and therapeutic effect of KM104 was comprehensively evaluated.【Result】 The cleavage rate of the bacteriophage KM104 in five serotypes of Salmonella spp.was 80.65% (50/62), in vitro it had good bacteriostatic effect and had no obvious pathogenic effect on chickens were found.The best treatment method:The therapeutic doses for Salmonella Pullorum, Salmonella Gallinarum and Salmonella Enteritidis were 7.96×10⁷, 1.26×10⁴ and 1.68×10⁷PFU/mL, respectively, and the survival rate of chicks was the highest (90%, 80% and 90%) by continuous feeding with high titer phage KM104 for 3 days.Compared with the untreated control group, it could effectively alleviate the clinical symptoms and organ damage of experimental chicks, and the weight gain increased significantly (P<0.05), and the bacterial excretion of anal swab, the bacterial load of spleen and organ index decreased significantly (P<0.05).【Conclusion】 Bacteriophage KM104 had broad-spectrum lytic activity against pathogenic Salmonella spp.of multiple serotypes, and had no obvious pathogenic effect on chickens were found.The method of feeding KM104 with high titer for 3 days before bacteria attack had the best preventive effect on chickens from being infected with Salmonella Pullorum, Salmonella Gallinarum and Salmonella Enteritis, and could thus be used for the development of Salmonella spp. biocontrol preparations.

Key words: Bacteriophage; lysis spectrum; Salmonella; chicken

CLC Number:

S852.65

CHEN Liyin, PENG Yating, YAN Hongya, CHEN Peifu, XIN Aiguo, LI Ke. Evaluation of the Prevention and Therapeutic Effect of Lytic Bacteriophage KM104 on Different Serotypes of Salmonella spp.from Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 278-291.

References

[1] GRIFFIN A J, MCSORLEY S J.Development of protective immunity to Salmonella, a mucosal pathogen with a systemic agenda[J].Mucosal Immunology, 2011, 4(4):371-382.
[2] KURTZ J R, GOGGINS J A, MCLACHLAN J B.Salmonella infection:Interplay between the bacteria and host immune system[J].Immunology Letters, 2017, 190:42-50.
[3] HAN J, GOKULAN K, BARNETTE D, et al.Evaluation of virulence and antimicrobial resistance in Salmonella enterica serovar Enteritidis isolates from humans and chicken and egg associated sources[J].Foodborne Pathogens and Disease, 2013, 10(12):1008-1015.
[4] RODRİGUEZ E C, DİAZ G P, MORENO J, et al.Laboratory surveillance of Salmonella Enterica from human clinical cases in Colombia 2005-2011[J].Enfermedades Infecciosas Y Microbiologia Clinica, 2016, 35(7):417-425.
[5] KUMAR Y, SINGH V, KUMAR G, et al.Serovar diversity of Salmonella among poultry[J].Indian Journal of Medical Research, 2019, 150(1):92-95.
[6] ADDWEBI T M, CALL D R, SHAH D H.Contribution of Salmonella Enteritidis virulence factors to intestinal colonization and systemic dissemination in 1-day-old chickens[J].Poultry Science, 2014, 93(4):871-881.
[7] SHIVAPRASAD H L.Fowl typhoid and pullorum disease[J].Revue Scientifique et Technique, 2000, 19(2):405-424.
[8] CELIS-ESTUPIAN A L D P, BATISTA D F A, CARDOZO M V, et al.Further investigations on the epidemiology of fowl typhoid in Brazil[J].Avian Pathology, 2017, 46(4):416-425.
[9] ARANAGA C, PANTOJA L D, MARTINEZ E A, et al.Phage therapy in the era of multidrug resistance in bacteria:A systematic review[J].International Journal of Molecular Sciences, 2022, 23(9):4577-4596.
[10] 贾盘兴.噬菌体分子生物学[M].北京:科学出版社, 2001. JIA P X.Phage Molecular Biology[M].Beijing:Science Press, 2001.(in Chinese)
[11] SHARMA S, CHATTERJEE S, DATTA S, et al.Bacteriophages and its applications:An overview[J].Folia Microbiologica, 2017, 62(1):17-55.
[12] BRUSSOW H, KUTTER E. Bacteriophages:Biology and Applications[M]. Florida:Chemical Rubber Company Press, 2005.
[13] GIAMARELLOU H.Multidrug-resistant Gram-negative bacteria:How to treat and for how long[J].International Journal of Antimicrob Agents, 2010, 36(2):50-54.
[14] 高雅, 王兆飞, 严亚贤.噬菌体治疗肺炎克雷伯菌感染的研究进展[J].微生物学通报, 2021, 48(9):3271-3280. GAO Y, WANG Z F, YAN Y X.Advances in the treatment of Klebsiella pneumoniae infection with bacteriophage therapy[J].Microbiology China, 2021, 48(9):3271-3280.(in Chinese)
[15] LE T S, NGUYEN T H, VO H P, et al.Protective effects of bacteriophages against Aeromonas hydrophila species causing Motile Aeromonas Septicemia (MAS) in striped catfish[J].Antibiotics, 2018, 7(1):16-26.
[16] ZHANG H, WAMG R, BAO H.Phage inactivation of foodborne Shigella on ready-to-eat spiced chicken[J].Poultry Science, 2013, 92(1):211-217.
[17] LIN Y W, CHANG R Y, RAO G G, et al.Pharmacokinetics/pharmacodynamics of antipseudomonal bacteriophage therapy in rats:A proof-of-concept study[J].Clinical Microbiology and Infection, 2020, 26(9):1229-1235.
[18] 何依蓉, 张奕杰, 杨伟, 等.鼠伤寒沙门菌烈性噬菌体的分离鉴定与生物学特性[J].畜牧兽医学报, 2021, 52(3):763-771. HE Y R, ZHANG Y J, YANG W, et al.Isolation, identification and biological properties of a lytic phage against Salmonella Typhimurium[J].Acta Veterinaria et Zootechnica Sinica, 2021, 52(3):763-771.(in Chinese)
[19] 唐宁, 殷茵, 任慧英, 等.沙门氏菌高效价宽谱噬菌体的分离纯化及生物学特性测定[J].中国畜牧兽医, 2021, 48(7):2577-2583. TANG N, YIN Y, REN H Y, et al.Isolation, purification and detection of biological characteristics of a high-titer and wide-spectrum Salmonella phage[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(7):2577-2583.(in Chinese)
[20] 陈冈, 郑盼盼, 高雅, 等.金黄色葡萄球菌噬菌体SP-2裂菌特性分析[J].中国兽医科学, 2021, 51(6):722-729. CHEN G, ZHENG P P, GAO Y, et al.The bacteriolytic characteristic of Staphylococcus aureus lytic phage SP-2[J].Veterinary Science in China, 2021, 51(6):722-729.(in Chinese)
[21] 顾兵, 张政, 李玉萍, 等.半数致死量及其计算方法概述[J].中国职业医学, 2009, 36(6):507-508. GU B, ZHANG Z, LI Y P, et al.Summary of median lethal dose and its calculation methods[J].China Occupational Medicine, 2009, 36(6):507-508.(in Chinese)
[22] DION M B, OECHSLIN F, MOINEAU S.Phage diversity, genomics and phylogeny[J].Nature Reviews Microbiology, 2020, 18(3):125-138.
[23] STONE E, CAMPBELL K, GRANT I, et al.Understanding and exploiting phage-host interactions[J].Viruses, 2019, 11(6):567-592.
[24] SORENSEN P E, NG D Y K, DUCHATEAU L, et al.Classification of in vitro phage-host population growth dynamics[J].Microorganisms, 2021, 9(12):2470-2486.
[25] GE H, XU Y, HU M, et al.Isolation, characterization and application in poultry products of a Salmonella-specific bacteriophage, S55[J].Journal of Food Protection, 2021, 84(7):1202-1212.
[26] 郝贺, 张永英, 钟翠红, 等.鸡源沙门菌噬菌体的生物学特性及其对雏鸡肠道结构的影响[J].中国兽医科学, 2022, 52(2):259-268. HAO H, ZHANG Y Y, ZHONG C H, et al.Biological characteristics of Salmonella phage from chicken and its effect on intestinal structure of chicks[J].Veterinary Science in China, 2022, 52(2):259-268.(in Chinese)
[27] VANDENHEUVEL D, LAVIGNE R, BRVSSOW H.Bacteriophage therapy:Advances in formulation strategies and human clinical trials[J].Annual Review of Virology, 2015, 2(1):599-618.
[28] TIAN M, HE X, FENG Y, et al.Pollution by antibiotics and antimicrobial resistance in livestock and poultry manure in China, and countermeasures[J].Antibiotics(Basel), 2021, 10(5):539-554.
[29] GORDILLO ALTAMIRANO F L, BARR J J.Phage therapy in the postantibiotic era[J].Clinical Microbiology Reviews, 2019, 32(2):66-84.
[30] 宋欣媛, 谭珊, 吴培福.昆明地区鸡源沙门氏菌的分离鉴定及耐药性分析[J].现代畜牧兽医, 2019, 4:1-5. SONG X Y, TAN S, WU P F.Isolation, identification and drug resistance of Salmonella from chicken in Kunming[J].Modern Journal of Animal Husbandry and Veterinary Medicine, 2019, 4:1-5.(in Chinese)
[31] 王喜, 李珂, 李廷翠, 等.75株蛋鸡源沙门菌的MLST分型与耐药性分析[J].畜牧兽医学报, 2022, 53(5):1626-1631. WANG X, LI K, LI T C, et al.MLST typing and drug resistance analysis of 75 Salmonella strains isolated from laying hens[J].Acta Veterinaria et Zootechnica Sinica, 2022, 53(5):1626-1631.(in Chinese)
[32] 李廷翠, 严红亚, 常志顺, 等.云南蛋鸡源肠炎沙门氏菌的分离鉴定及生物学特性[J].微生物学通报, 2021, 48(1):82-92. LI T C, YAN H Y, CHANG Z S, et al.Isolation, identification and characterization of Salmonella Enteritis from Yunnan laying hens[J]. Microbiology China, 2021, 48(1):82-92.(in Chinese)
[33] 王馨锐, 李继桐, 韩丽丽, 等.鸡源大肠杆菌噬菌体vB_EcoM-E33的分离鉴定和基因组特征及其治疗效果评价[J].微生物学通报, 2022, 49(12):5009-5021. WANG X R, LI J T, HAN L L, et al.Chicken-derived coliphage vB_EcoM-E33:Isolation, identification, genomic characterization and evaluation of its therapeutic effect on colibacillosis[J].Microbiology China, 2022, 49(12):5009-5021.(in Chinese)
[34] 袁玉玉.鸡白痢沙门氏菌噬菌体的分离鉴定及其初步应用[D].长春:吉林大学, 2016. YUAN Y Y.The isolation and identification of Salmonella pullorum bacteriophage and its preliminary application[D].Changchun:Jilin University, 2016.(in Chinese)
[35] JAISWAL A, KOLEY H, GHOSH A, et al.Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model[J].Microbes and Infection, 2013, 15(2):152-156.
[36] 李托, 李陇平, 董书伟, 等.绒山羊源大肠杆菌噬菌体φPTK的分子生物学特性及对小鼠大肠杆菌感染的防治效果[J].微生物学通报, 2023, 50(8):3479-3494. LI T, LI L P, DONG S W, et al.Molecular biological characteristics of cashmere goat-derived Escherichia coli phage φPTK and the effect on mice infected with E.coli[J].Microbiology China, 2023, 50(8):3479-3494.(in Chinese)
[37] 范亚娟, 袁宸, 马喆.沙门氏菌噬菌体混合制剂研制及体外杀菌效果评价[J].扬州大学学报(农业与生命科学版), 2021, 42(2):79-85. FAN Y J, YUAN C, MA Z.Development of Salmonella bacteriophage mixture and in vitro sterilization effect[J].Journal of Yangzhou University (Agricultural and Life Science Edition), 2021, 42(2):79-85.(in Chinese)
[38] PEREIRA C, MOREIRINHA C, LEWICKA M, et al.Bacteriophages with potential to inactivate Salmonella Typhimurium:Use of single phage suspensions and phage cocktails[J].Virus Research, 2016, 220:179-192.

Evaluation of the Prevention and Therapeutic Effect of Lytic Bacteriophage KM104 on Different Serotypes of Salmonella spp.from Chickens

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