基于免疫信息学技术的雏沙门菌多表位疫苗构建

doi:10.16431/j.cnki.1671-7236.2024.01.031

Abstract

Abstract: 【Objective】 This study was aimed to design multi-epitope vaccine against IpaJ protein of Salmonella Pullorum and provide a new vaccine for purifying chicken dysentery.【Method】 In this study, IEDB was used to predict the major histocompatibility complex class Ⅰ (MHC Ⅰ) molecular binding epitopes of Salmonella Pullorum IpaJ protein in T lymphocytes.NetMHCIIpan 4.0 Server was used to predict T lymphocytes MHC Ⅱ molecular binding epitopes.B lymphocyte epitope was predicted by IEDB.After the antigenicity of the selected epitopes was evaluated by VaxiJen v 2.0, the qualified epitopes were concatenated into multi-epitope vaccine by flexible linker.Antigenicity, physicochemical properties, N-glycosylation sites, secondary structure and tertiary structure of the constructed multi-epitope vaccine were predicted.Molecular docking was used to evaluate the binding ability of the multi-epitope vaccine to the immune receptor.Immune simulation was used to evaluate the immune effect of the multi-epitope vaccine.Finally, the codon was optimized for cloning expression.【Result】 After screening, the constructed multi-epitope vaccine MEV-IpaJ contained 4 MHC Ⅰ, 4 MHC Ⅱ and 8 B lymphocyte epitope dominance.The relative molecular weight of the multi-epitope vaccine MEV-IpaJ was 28.18 ku, which was a stable hydrophilic protein and had good antigenicity.There were four potential N-glycoylation sites, including α-helix, extended strand, beta turn and random coil accounted for 20.38%, 19.23%, 8.08% and 52.31%, respectively.Ramachandran mapping of tertiary structure showed that the dominant region contained 89.9% of the residual base, and the residual base of the dominant region increased to 94.0% after refinement.The mapping of tertiary structure prominent epitope also proved that the multi-epitope vaccine had good immunogenicity, and molecular docking showed that the MEV-IpaJ could dock with Toll-like receptor 2 (TLR2) and TLR4 protein molecules.The results of immune simulation showed that the MEV-IpaJ had a good immune response and could improve the expression of some cytokines.Codon optimization ensured the efficient and stable expression of the MEV-IpaJ in E.coli K12 expression system.【Conclusion】 The constructed multi-epitope vaccine MEV-IpaJ could be effectively expressed and might induce strong T cell and B cell immune responses.This study provided a new method for the design of multi-epitope vaccine of Salmonella Pullorum, and provided theoretical basis and data support for the research and development of multi-epitope vaccine of Salmonella Pullorum.

Key words: Salmonella Pullorum; immunoinformatics; epitope; multi-epitope vaccine

CLC Number:

S852.4

TAN Ju, WANG Yongjuan, GUO Guangfu, ZHAO Changjing, XIA Aihong, LI Juyin, WU Minqiu, WANG Yuhang, QIN Qiuping. Construction of a Multi-epitope Vaccine Against Salmonella Pullorum by Immunoinformatics Approach[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 312-322.

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Construction of a Multi-epitope Vaccine Against Salmonella Pullorum by Immunoinformatics Approach

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