CTC法结合体外受精比较不同方法对绵羊精子体外获能的影响

›› 2007, Vol. 34 ›› Issue (8): 31-34.

• 遗传繁育 • Previous Articles Next Articles

Comparison of CTC Staining and in Vitro Fertilization among Different Ovine Sperm Capacitation Methods

LIU Lieqin1, LIU Chousheng2, GUO Yong1, NI Hemin1, ZHANG Hong1,YANG Feige1, LIU Yunhai1

1.Department of Animal Science & Technology, Beijing University of Agriculture, Beijing 102206,China;2.The Center of Preservation and Utilization of Germplasm Resources of Animal Husbandry and Forage Grass,National Husbandry and Veterinary Service, Beijing 100094,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-20 Published:2007-08-20

Abstract

Abstract: In this experiment, the discrepancy was compared among the “Swim-up” , Induction by A23187, Percoll and Incubation after Percoll methods for ovine sperm capacitation in vitro with chlortetracycline fluorescence (CTC) staining and in vitro fertilization. The results showed that the rates of sperm capacitation and the cleavage treated by Swim-up method were higher than the others. It might be suggested that the Swim-up method is exactly suitable for the application of in vitro fertilization of sheep. 

Key words: ovine; sperm capacitation; CTC; in vitro fertilization

CLC Number:

Q813

LIU Lieqin;LIU Chousheng;GUO Yong;NI Hemin;ZHANG Hong;YANG Feige;LIU Yunhai. Comparison of CTC Staining and in Vitro Fertilization among Different Ovine Sperm Capacitation Methods[J]. , 2007, 34(8): 31-34.

[1]	HU Junhao, LIU Jibing, ZHOU Jiali, MA Yue, ZHAO Hongxi. Progress on in vitro Culture of Bovine Coccidia [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 700-708.
[2]	HE Lin, HAN Diangang, LI Jing, DU Xuan, ZHANG Jiaxiang, XIN Jige. The Progress of the Detection Methods of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1593-1602.
[3]	ZHEN Zhen, WANG Mei, WANG Yimin, HU Debao, ZHANG Linlin, LI Xin, GUO Yiwen, GUO Hong, DING Xiangbin. Effect of RNA Methylation Transfer Enzyme METTL3 on the Proliferation and Myogenic Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1025-1036.
[4]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[5]	XIANG Decai, LI Shuiying, ZHANG Bin, ZHANG Yan, LIANG Jiachong, LYU Chunrong, HONG Qionghua, QUAN Guobo, WU Guoquan. The Study on the Role of Fetal Bovine Serum Supplementation During Postwarming Culture on Vitrified Porcine Parthenogenetic Blastocysts [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 606-615.
[6]	HAN Mengli, ZHANG Qian, ZHAO Wenjuan, ZHANG Xingxing, WU Tongzhong, HUANG Xin, ZHONG Fagang. Advances on Molecular Biology and Pathogenesis of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4632-4645.
[7]	ZHANG Haobo, ZHAO Shanjiang, WANG Huan, XU Xi, FENG Zhiqiang, WANG Wanjie, GONG Jianfei, PANG Yunwei, DU Weihua, ZHU Huabin. Effects of Addition of Insulin-Transferrin-Sodium Selenite on Effect of Production, Hypothermic Storage and Routine Freezing of Bovine in vitro Embryos [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4077-4084.
[8]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[9]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.
[10]	SHAO Baihui, JIANG Dongjun, XIE Yimeng, LIU Siyu, SHAO Ziyi, ZHANG Zecai, JI Hong, ZHU Zhanbo. Molecular Docking Prediction of Artemisinin and NS5B Protein of Bovine Viral Diarrhea Virus and Its Antiviral Effect [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3581-3588.
[11]	DENG Li, LI Jie, LIU Baoguo, HANG Bolin. Molecular Design and Antimicrobial Activity Analysis of Antimicrobial Peptides Derived from Bovine Hemoglobin β-subunit [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3589-3598.
[12]	WANG Yuchen, TIAN Guangyuan, ZHOU Yaping, GUO Ting, ZHAO Hongmei, SUN Yajie, ZHAO Fengmiao, BIAN Yuchen, YU Jialiang, HAO Yongqing. Prokaryotic Expression of BPIV3 NP Protein and Its Immunoenhancing Effect on the BPIV3 Inactivated Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3122-3130.
[13]	WANG Wei, HAN Huihui, DING Naizheng, HE Chengqiang. Prokaryotic Expression of Bovine Viral Diarrhea Virus E0 Protein and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3131-3139.
[14]	WANG Huihui, FENG Xili, PU Feiyang, LI Yicong, WANG Mengzhu, ZHOU Xiaokai, ZHAO Zeyang, MA Zhongren, LI Zhuo, MA Xiaoxia. Prokaryotic Expression and Antigen Epitope for Non-structural Protein NS5A of BVDV Isolated from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3180-3189.
[15]	LU Wengeng, SI Linqing, TIAN Chunyu, YUAN Siqi, XIE Hexin, ZHOU Jiwei, JIN Jidong, HAN Yinghao. Establishment of Oxidative Stress Model of Bovine Endometrial Epithelial Cells and the Effects of Bovine Adipose-derived Mesenchymal Stem Cells on Cell Migration [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2506-2514.

Comparison of CTC Staining and in Vitro Fertilization among Different Ovine Sperm Capacitation Methods

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments