褪黑素基础上补喂来曲唑和N-甲基-D-天冬氨酸对母羊血浆繁殖激素水平的影响

doi:10.16431/j.cnki.1671-7236.2018.12.018

Abstract

Abstract:

This experiment was conducted to study the effects of feeding melatonin (MLT) on the breeding ewes in the off-season and feeding letrozole (LE) and N-methyl-D-aspartate (NMDA) on the basis of MLT,and to provide reference for the development and utilization of regulating ewes estrus drugs.Forty health multiparous Suffolk ewes with the age of (3.0±0.5) years old and the average weight of 76.23 kg±12.25 kg were randomly divided into 4 groups,control group and test groups Ⅰ,Ⅱ and Ⅲ,10 in each group.Control group:Basal diet;Test groupⅠ:Basal diet+ MLT 10 mg/d;Test groupⅡ:Basal diet+MLT 10 mg/d+LE 7 mg/d;Test group Ⅲ:Basal diet+MLT 10 mg/d+LE 7 mg/d+NMDA 300 mg/d.The results showed that,compared with the control group,the levels of FSH,E₂,E₃ and DHT in ewes plasma extremely significantly increased (P<0.01),and the level of T significantly decreased (P<0.05) in test group Ⅰ;Plasma MLT and LH levels were extremely significantly increased (P<0.01) and the FSH level was significantly increased (P<0.05) in test groupⅡ;In test group Ⅲ,the levels of MLT and GnRH in the plasma of the ewe were significantly increased (P<0.05).Compared with test groupⅠ,the MLT in the plasma of test group Ⅱ was significantly increased (P<0.05),and the LH and T levels were extremely significantly increased (P<0.01).Compared with test groups Ⅰ and Ⅱ,the FSH level was extremely significantly lower in test group Ⅲ (P<0.01).In summary,MLT and LE had a promoting effect on the release of reproductive-related hormones in ewes,and the combination of the two could achieve the effect of regulating the hormone levels related to reproductive growth of ewes;The combined application of MLT,LE and NMDA had a poor effect on the regulation of reproductive hormones in ewes.

Key words: melatonin; letrozole; N-methyl-D-aspartate; reproductive hormones; ewe

CLC Number:

S826

ZHAO Guodong, ZANG Changjiang, WANG Gen, XIE Jinglong, YANG Jiantao, TANG Xuemei, ZHANG Juanli, YANG Kailun. Effects of Supplement with Letrozole and N-methyl-D-aspartate on Plasma Reproductive Hormone Levels of Ewes on the Basis of Melatonin Supplementation[J]. , 2018, 45(12): 3455-3462.

References

[1] RUDOLPH L M,BENTLEY G E,CALANDRA R S,et al.Peripheral and central mechanisms involved in hormonal control of male and female reproduction[J].Journal of Neuroendocrinology,2016,28(7):12405.
[2] CELI I,GATICA M C,GUZMAN J L,et al.Influence of the male effect on the reproductive performance of female Payoya goats implanted with melatonin at the winter solstice[J].Animal Reproduction Science,2013,137(3-4):183-188.
[3] BITTMAN E L,DEMPSEY R J,KARSCH F J.Pineal melatonin secretion drives the reproductive response to daylength in the ewe[J].Endocrinology,2013,113(6):2276-2283.
[4] 刘晓燕,郭霞珍,许筱颖,等.反季节光照模拟对大鼠血清褪黑素及性腺轴激素的影响[J].中华中医药学刊,2009,1:69-71. LIU X Y,GUO X Z,XU X Y,et al.Effect of inverse seasonal light modeling to the melatonin and sexualhormone in blood serum of rat[J].Chinese Archives of Traditional Chinese Medicine,2009,1:69-71.(in Chinese)
[5] ABECIA J A,FORCADA F,VÁZQUEZ M I,et al.Effect of level of energy and exogenous melatonin on pituitary responsiveness to GnRH in Rasa Aragonesa ewes during anestrus and the reproductive season[J].Genetics Selection Evolution,2013,45:341-343.
[6] WANG L,ZHUO Z Y,SHI W Q,et al.Melatonin promotes superovulation in sika deer (Cervus nippon)[J].International Journal of Molecular Sciences,2014,15(7):12107-12118.
[7] 庄另发,赵莞,龚炜,等.不同剂量来曲唑联合氯米芬对不孕多囊卵巢综合征患者助孕和生殖激素变化的影响[J].中国妇幼保健,2015,30(16):2593-2595. ZHUANG L F,ZHAO W,GONG W,et al.Effects of different doses of letrozole combined with clomifene on assisted reproduction and reproductive hormoneschanges of patients with polycystic ovary syndrome[J].Maternal and Child Health Care of China,2015,30(16):2593-2595.(in Chinese)
[8] GAY V L,PLANT T M.N-methyl-D,L-aspartate elicits hypothalamic gonadotropin-releasing hormone release in prepubertal male rhesus monkeys (Macacamulatta)[J].Endocrinology,1987,120(6):2289-2296.
[9] KENNAWAY D J,SEAMARK R F.Circulating levels of melatonin following its oral administration or subcutaneous injection in sheep and goats[J].AustralianJournal of Biological Sciences,1980,33(3):349-353.
[10] 王松,高腾云,李新建.褪黑激素在动物生产上的研究进展[J].家畜生态学报,2007,28(4):5-8. WANG S,GAO T Y,LI X J.Advance of melatonin on animal production[J].Journal of Domestic Animal Ecology, 2007,28(4):5-8.(in Chinese)
[11] 郭婷婷.蒙古羊褪黑素受体基因(MTNR1a) mRNA表达差异的季节性研究[D].兰州:甘肃农业大学,2009. GUO T T.Study on the seasonal rhythm of differential expression of melatonin receptor (MTNR1a) mRNA in Mongolia sheep[D].Lanzhou:Gansu Agricultural University,2009.(in Chinese)
[12] 王淑娟,刘文举,王立克,等.褪黑激素对雌性动物生殖系统调节作用的研究进展[J].江苏农业科学,2016,44(6):15-20. WANG S J,LIU W J,WAN L K,et al.The regulation of the reproductive system of female animals by melatonin research progress[J].Jiangsu Agricultural Sciences,2016,44(6):15-20.(in Chinese)
[13] 马友记,李发弟,张利.褪黑素对动物生殖调控机理的研究进展[J].中国草食动物,2007,增刊:85-88. MA Y J,LI F D,ZHANG L.Melatonin on animalreproductive regulation mechanism research progress[J].China Herbivores,2007,suppl:85-88.(in Chinese)
[14] MALPAUX B,DAVAU A,MAURICE-MANDON F,et al.Evidence that melatonin acts in the premammillary hypothalamic area to control reproduction in the ewe:Presence of binding sites and stimulation of luteinizing hormone secretion by in situ microimplant delivery[J].Endocrinology,1998,139(4):1508-1516.
[15] 迟素敏.内分泌生理学[M].西安:第四军医大学出版社,2006. CHI S M,Endocrine Physiology[M].Xi'an:The Tourth Military Medica Luniversity Press,2006.(in Chinese)
[16] CASAO A,PÉREZPÉ R,ABECIA J A,et al.The effect of exogenous melatonin during the non-reproductive season on the seminal plasma hormonal profile and the antioxidant defence system of Rasa Aragonesarams[J].Animal Reproduction Science,2013,138(3-4):168-174.
[17] REKIK M,TABOUBI R,SALEM I B,et al.Melatonin administration enhances the reproductive capacity of young rams under a Southern Mediterranean environment[J].Animal Science Journal,2015,86(7):666-672.
[18] MEIKLE A W,MAZER N A,MOELLMER J F,et al.Enhanced transdermal delivery of testosterone across nonscrotal skin produces physiological concentrations of testosterone and its metabolites in hypogonadal men[J].Journalof Clinical Endocrinology & Metabolism,1992,74(3):623-628.
[19] MICHEL G,BAULIEU E E.Androgen receptor in rat skeletal muscle:Characterization and physiologicalvariations[J].Endocrinology,1980,107(6):2088-2098.
[20] WANG Y F,SUN X F,HAN Z L,et al.Protective effects of melatonin against nicotine-induced disorderof mouse early folliculogenesis[J].Aging,2018,10(3):463-480.
[21] TANAVDE V S,MAITRA A.In vitro modulation of steroidogenesis and gene expression by melatonin:A study with porcine antral follicles[J].Endocrine Research Communications,2003,29(4):399-410.
[22] OSHIMA C T F.Pinealectomy changes rat ovarian interstitial cell morphology and decreases progesterone receptor expression[J].Gynecological Endocrinology,2003,17(2):115-123.
[23] LANGFORD G A.Influence of PMSG and time of artificial insemination on fertility of progestogen-treated sheep in confinement[J].Journal of Animal Science,1982,54(6):1205-1211.
[24] 赵国栋,王根,谢景龙,等.补饲醋酸甲羟孕酮基础上分别进行前列腺素、N-甲基-D-天冬氨酸和来曲唑处理对母羊血浆繁殖激素水平的影响[J].中国畜牧兽医,2017,44(11):3229-3235. ZHAO G D,WANG G,XIE J L,et al.Effects of prostaglandin,N-methyl-D-aspartate and letrozole on ewes plasma levels of reproductive hormones were evaluated on the basis of medroxyprogesterone acetate supplementation[J].China Animal Husbandry &Veterinary Medicine,2017,44(11):3229-3235.(in Chinese)
[25] 史军红.高原甘加型藏羊发情周期垂体FSH和LH细胞的表达及其激素分泌的动态变化研究[D].兰州:甘肃农业大学,2016. SHI J H.The dynamic change expression and secrete hormones of FSH and LH cells in the pituitary of Ganjia Tibetan sheep estrous cycle[D].Lanzhou:Gansu Agricultural University,2016.(in Chinese)
[26] 张希牧,李军,田永路,等.芳香化酶在人类乳腺癌、睾丸癌发生过程中的作用[J].实验动物科学,2009,26(6):62-63. ZHANG X M,LI J,TIAN Y L,et al.Aromatase in human breast cancer and its effect in the process of testicular cancer[J].Laboratory Animal Science and Administration,2009,26(6):62-63.(in Chinese)
[27] KAWAKAMI E,HIRANO T,HORI T,et al.Improvement in spermatogenic function after subcutaneous implantation of a capsule containing an aromatase inhibitor in four oligozoospermic dogs and one azoospermic dog with high plasma estradiol-17β concentrations[J].Theriogenology,2004,62(1-2):165-178.
[28] GOSS P E,INGLE J N,SILVANA M,et al.A randomized trial of letrozole in postmenopausal women after five years of tamoxifen therapy for early-stage breast cancer[J].New England Journal of Medicine,2003,349(19):1793-1802.
[29] GORE A C,ROBERTS J L.Regulation of gonadotropin-releasing hormone gene expression by the excitatory amino acids kainic acid and N-methyl-D,L-aspartate in the male rat[J].Endocrinology,1994,134(5):2026-2031.
[30] GAZAL S,KOUAKOU B,AMOAH E A,et al.Effects of N-methyl-D,L-aspartate on LH,GH,and testosterone secretion in goat bucks maintained under long or short photoperiods[J].Journal of Animal Science,2002,80(6):1623-1628.
[31] SCACCHI P,CARBONE S,SZWARCFARB B,et al.Interactions between GABAergic and serotoninergic systems with excitatory amino acid neurotransmission in the hypothalamic control of gonadotropin secretion in prepubertal female rats[J].Developmental Brain Research,1998,105(1):51-58.
[32] HONARAMOOZ A,CHANDOLIA R K,BEARD A P,et al.Excitatory amino acid regulation of gonadotropin secretion in prepubertal heifer calves[J].Biology of Reproduction,1998,59(5):1124-1130.

Effects of Supplement with Letrozole and N-methyl-D-aspartate on Plasma Reproductive Hormone Levels of Ewes on the Basis of Melatonin Supplementation

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