牛乳腺上皮细胞SNAT2对氨基酸调节乳合成的影响

doi:10.16431/j.cnki.1671-7236.2018.08.010

Abstract

Abstract:

This experiment was aimed to investigate the effect of amino acid transporter the sodium-dependent neutral amino acid transporter 2 (SNAT2) on amino acid-stimulated milk protein and milk fat synthesis in bovine mammary epithelial cell (BMEC).After stimulating BMEC with methionine (Met),lysine (Lys) and leucine (Leu),the expression level of SNAT2 and β-casein gene was detected by Real-time quantitative PCR and Western blotting,and the secretion of triglyceride in culture supernatant was detected using BMEC triglyceride kit.The N-flag-SNAT2 eukaryotic expression vector and SNAT2 siRNA were transfected into the cells for SNAT2 overexpression and knockdown experiments,respectively.The expression level of SNAT2,β-casein,the mammalian target of rapamycin (mTOR),and the content of triglyceride in the culture supernatant of BMEC were detected by Western blotting and triglyceride kit,respectively.The results showed that all three amino acids (Met,Lys and Leu) could significantly increase the content of milk protein and milk fat secreted by BMEC and activate the mTOR signaling pathway.Met and Lys also up-regulated significantly the expression of SNAT2 gene.SNAT2 regulated positively the synthesis of milk protein and milk fat in BMEC,and activated the mTOR signaling pathway,indicating that amino acid activation of the mTOR signaling pathway was mediated through SNAT2,which in turn regulated BMEC milk protein and milk fat synthesis.

Key words: amino acids; SNAT2 gene; mTOR; milk protein; milk fat

CLC Number:

S823

MENG Chunyu, HUANG Xin, LIU Lijie, CHEN Dongying, GAO Xuejun. Effects of SNAT2 on Amino Acid-stimulated Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells[J]. , 2018, 45(8): 2119-2127.

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Effects of SNAT2 on Amino Acid-stimulated Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells

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