摩拉水牛及德宏水牛瘤胃产甲烷菌多样性比较

doi:10.16431/j.cnki.1671-7236.2018.02.010

Abstract

Abstract:

The objective of this study was to investigate the diversity of ruminal archaea in Murrah buffaloes and Dehong buffaloes through 16S rDNA library-based analysis.Three female Murrah buffaloes and three female Dehong buffalo were used in this study and rumen liquid were taken by stomach tube. Total DNA were extracted by phenol-chloroform-isopentanol extraction method. Primers of Met86F/Met1340R were used to amplify the 16S rDNA of archaea for the construction of library. The results showed that 96 sequences were obtained from each library of Murrah buffaloes and Dehong buffaloes. Based the 97% similarity,sequences of Murrah buffaloes and Dehong buffaloes were assigned to 27 and 14 OTUs,respectively.For Dehong buffaloes,82 sequences showed ≥ 97% similarity to known species,14 sequences had the similarity to known species in the range of 89% to 97%. For Murrah buffaloes,94 sequences showed ≥ 97% similarity to known species,only two sequences had 94% (< 97%) similarity to known species. Phylogenetic analysis indicated that these sequences were assigned into two large clades. There were 13 OTUs representative sequences from Dehong buffaloes and 7 OTUs representative sequences from Murrah buffaloes assigned into the same clade on the top of the phylogenetic tree and far from all known species. They might be represented new genus or species in Methanobacteriales order in the rumen of Murrah buffaloes and Dehong buffaloes. The SGMT clade sequence and RO clade sequence percentage in Dehong buffaloes and Murrah buffaloes were 83.3% and 9.4%, 51.0% and 9.4%,respectively.It could be concluded that Methanobacteriales order was the dominant archaea in the rumen of Murrah buffaloes and Dehong buffaloes. There were more unknown methanogen sequences and SGMT clade sequences in Dehong buffaloes than Murrah buffaloes under the same diet.

Key words: Murrah buffalo; Dehong buffalo; rumen; methanogen; diversity

CLC Number:

S823

YANG Chengjian, LIANG Xin, LI Lili, LI Mengwei, LIANG Xianwei, PENG Kaiping, GUO Yanxia, TANG Qingfeng. 16S rDNA Library-based Analysis of Ruminal Archaeal Diversity in Murrah Buffaloes and Dehong Buffaloes[J]. , 2018, 45(2): 365-374.

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16S rDNA Library-based Analysis of Ruminal Archaeal Diversity in Murrah Buffaloes and Dehong Buffaloes

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