›› 2017, Vol. 44 ›› Issue (12): 3663-3669.doi: 10.16431/j.cnki.1671-7236.2017.12.038

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Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Anhui

SHEN Xue-huai, ZHANG Dan-jun, PAN Xiao-cheng, ZHAO Rui-hong, DAI Yin, HU Xiao-miao   

  1. Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2017-05-18 Online:2017-12-20 Published:2017-12-20

Abstract:

In order to investigate the situation of predominant strain and antibiotic resistance of avian Salmonella in Anhui province, 42 strains Salmonella were isolated and identified from 116 suspected samples by culture characteristics, microscopic examination, biochemical test, PCR and serological identification,then drug resistance was analyzed by Kindy-Bauer method. There were 5 groups of 11 serotypes in 42 strains Salmonella, of which 30 strains belonged to B serogroup, 2 strains to A serogroup, 7 strains to D serogroup, 2 strains to C1 serogroup, 1 strain to C2 serogroup, and Salmonella Typhimurium of B serogroup were the predominant serotypes. All Salmonella isolates resistant rates to amikacin, levofloxacin and ciprofloxacin were less than 20%, and the resistance rates to ampicillin, streptomycin, furazolidone, spectinomycin, cotrimoxazole and doxycycline were more than 50%, while resistance rate to ampicillin was 97.62%, and 41 isolates had multiple drug resistance. The results showed that Salmonella Typhimurium was the predominant serotypes of avian Salmonella in Anhui, and isolates showed multiple drug resistance. This study would provide the data basis for the comprehensive prevention and treatment of clinical medicine of avian Salmonella.

Key words: avian Salmonella; isolation and identification; serotype; drug resistance

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