猪嵴病毒病原特性及检测技术研究进展

doi:10.16431/j.cnki.1671-7236.2017.12.011

Abstract

Abstract:

Porcine kobuvirus (PKV) is a member of the newly detected in the genus Kobuvirus, family Picornaviridae in healthy pigs and diarrhea pigs. It may cause diarrhea of pigs and cause significant economic losses to pig industry. At present, the studies find that, PKV is widely distributed in pigs and has been detected in diarrhea and clinically healthy pigs, and the positive rate of the virus varies from 3.9% to 100.0%. A typical porcine kobuvirus virion is 30 nm in diameter, and its genome is 8 120 bp in length and contains a single open reading frame (ORF) encoding a protein of 2 488 amino acids. The genome organization is typical for picornaviruses:A 5' UTR, a leader (L) protein, structural proteins P1 (VP0, VP3 and VP1), non-structural proteins P2 (2A, 2B and 2C) and P3 (3A, 3B, 3C and 3D), a 3' UTR and a Poly (A) tail. There are 30 amino acid deletions in the 2B coding region of the porcine kobuvirus, VP1 protein is the most frequent variant of the small RNA virus, structural protein, which contains the main epitope, can promote the body to produce neutralizing antibodies. At present, the methods of detecting PKV are reverse transcription polymerase chain reaction (RT-PCR), TaqMan probe Real-time RT-PCR and reverse transcription-loop mediated amplification (RT-LAMP). In this paper, a brief overview of the taxonomy, epidemiological profile, genome structure, genetic characteristics and detection techniques of PKV was made, so as to provide references for the further study and understand PKV.

Key words: porcine kobuvirus (PKV); epidemiology; genome structure; genetic characteristics; detection technique

CLC Number:

S858.28

HUANG Xiao-xing, WANG Xian, JIN Wen-jie. Research Advances in Pathogenicity and Detection Techniques of Porcine Kobuvirus[J]. , 2017, 44(12): 3466-3472.

References

[1] 张莎. 猪嵴病毒流行病学调查及CH/HZ/2011株全基因序列分析[D]. 哈尔滨:东北农业大学, 2013.
[2] 王振玲, 段跃强, 王金秋,等. 北京地区仔猪大肠杆菌血清型鉴定及其毒力因子检测[J]. 中国畜牧兽医, 2017, 44(5):1302-1308.
[3] Zhang Q, Hu R, Tang X, et al. Occurrence and investigation of enteric viral infections in pigs with diarrhea in China[J].Archives of Virology, 2013,158(8):1631-1636.
[4] Reuter G,Boros A, Pankovics P. Kobuviruses-A comprehensive review[J]. Reviews in Medical Virology, 2011, 21(1):32-41.
[5] Boros Á, Nemes C, Pankovics P, et al. Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)[J]. Journal of General Virology, 2012, 93(10):2171-2182.
[6] Knowies N J, Hovi T, Hyypia T, et al. Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses:Picornaviridae[M]. San Diego:Elsevier Academic Press, 2012.
[7] Khamrin P, Maneekarn N, Okitsu S, et al. Epidemiology of human and animal kobuviruses[J]. Virus Disease, 2014, 25(2):195-200.
[8] Ribeiro J, deArrudaLeme R, Alfieri A F, et al. High frequency of Aichivirus C (porcine kobuvirus) infection in piglets from different geographic regions of Brazil[J]. Tropical Animal Health and Production, 2013, 45(8):1757-1762.
[9] Reuter G,Boldizsar A, Kiss I, et al. Candidate new species of Kobuvirus in porcine hosts[J]. Emerging Infectious Diseases, 2008, 14(12):1968-1970.
[10] Khamrin P, Maneekarn N, Hidaka S, et al. Molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in Japan[J]. Infection, Genetics and Evolution, 2010, 10(7):950-954.
[11] Wang C, Lan D, Hua X. Porcine kobuvirus from pig stool specimens in Shanghai, China[J]. Virus Genes, 2011, 43(3):350-352.
[12] An D J, Jeoung H Y, Jeong W, et al. Porcine kobuvirus from pig stool in Korea[J]. Virus Genes, 2011, 42(2):208-211.
[13] Khamrin P, Maneekarn N, Kongkaew A, et al. Porcine kobuvirus in piglets, Thailand[J]. Emerging Infectious Diseases, 2009, 15(12):2075-2076.
[14] Amimo J O, Okoth E, Junga J O, et al. Molecular detection and genetic characterization of kobuviruses and astroviruses in asymptomatic local pigs in East Africa[J]. Arch Virol, 2014, 159(6):1313-1319.
[15] Barry A F, Ribeiro J, Alfieri A F, et al. First detection of kobuvirus in farm animals in Brazil and the Netherlands[J]. Infection, Genetics and Evolution, 2011, 11(7):1811-1814.
[16] Verma H, Mor S K, Abdel-Glil M Y, et al. Identification and molecular characterization of porcine kobuvirus in U. S. swine[J]. Virus Genes, 2013, 46(3):551-553.
[17] DiProfio F, Ceci C, Di Felice E, et al. Molecular detection of porcine kobuviruses in Italian swine[J]. Research in Veterinary Science, 2013, 95(2):782-785.
[18] Yamashita T, Kobayashi S,Sakac K, et al. Isolation of cytopathic small round viruses with BS-Cl cells from patients with gastroenteritis[J]. The Journal of Infectious Diseases, 1991, 164(5):954-957.
[19] Reuter G,Boros Á, Pankovics P, et al. Kobuvirus in domestic sheep, Hungary[J]. Emerging Infectious Diseases, 2010, 16(5):869-870.
[20] Li L, Victoria J G, Wang C, et al. Bat guano virome:Predominance of dietary viruses from insects and plants plus novel mammalian viruses[J]. Journal of Virology, 2010, 84(14):6955-6965.
[21] Phan T G, Kapusinszky B, Wang C, et al. The fecal viral flora of wild rodents[J]. PLoS Pathogens, 2011, 7(9):e1002218.
[22] Li L,Pesavento P A, Shan T, et al. Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses[J]. Journal of General Virology, 2011, 92(11):2534-2541.
[23] Lee M H, Jeoung H Y, Lim J A, et al. Kobuvirus in South Korean Black goats[J]. Virus Genes, 2012, 45(1):186-189.
[24] Chung J Y, Kim S H, Kim Y H, et al. Detection and genetic characterization of feline kobuviruses[J]. Virus Genes, 2013, 47(3):559-562.
[25] Smits S L, Raj V S, Oduber M D, et al. Metagenomic analysis of the ferret fecal viral flora[J]. PLoS One, 2013, 8(8):e71595.
[26] Wang E,Yang B, Liu W, et al. Complete sequencing and phylogenetic analysis of porcine kobuvirus in domestic pigs in Northwest China[J]. Archives of Virology, 2014, 159(9):2533-2535.
[27] Peng Q, Song D P, Huang D Y, et al. Complete genome sequence of a porcine kobuvirus variant strain from Jiangxi, China[J]. Genome Announcements, 2017, 5(5):e01580-16.
[28] Yamashita T, Ito M,Kabashima Y, et al. Isolation and characterization of a new species of kobuvirus associated with cattle[J]. Journal of General Virology, 2003, 84(Pt 11):3069-3077.
[29] Lu L, Van Dung N, Bryant J E, et al. Evolution and phylogeographic dissemination of endemic porcine picornaviruses in Vietnam[J]. Virus Evol, 2016, 2(1):vew001.
[30] Wang C, Lan X, Yang B. Molecular epidemiological investigation of porcine kobuvirus and its coinfection rate with pedv and sav in Northwest China[J]. Biomed Research International, 2016, 2016(12):7590569.
[31] Sasaki J, Kushhara Y, Maeno Y, et al. Construction of an infectious cDNA clone of Aichi virus (a new member of the family Picornaviridae) and mutational analysis of a stem-loop structure at the 5'end of the genome[J]. Journal of Virology, 2001, 75(17):8021-8030.
[32] 朱庆贺, 陈曦,王观悦,等. 猪嵴病毒流行概况及诊断方法的研究进展[J]. 黑龙江畜牧兽医, 2016, 11:66-69.
[33] Yu J M, Xu Z Q, Li B W, et al. Analysis and characterization of the complete genome of a member of a new species of kobuvirus associated with swine[J]. Arch Virol, 2011, 156(5):747-751.
[34] 王琛,兰喜,祝俊鹏,等. 猪嵴病毒3C蛋白的原核表达及其结构分析[J]. 中国畜牧兽医, 2016, 43(4):845-853.
[35] 张永军, 胡永浩. 福建地区2株猪嵴病毒VP1基因的克隆及遗传进化分析[J]. 安徽农业科学, 2016, 44(14):149-151.
[36] Liu X, Oka T, Wang Q. Genomic characterization of a US porcine kobuvirus strain[J]. Arch Microbiol, 2015, 197(8):1033-1040.
[37] Zhu X, Wang Y, Chen J, et al. Development of TaqMan Real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus[J]. Journal of Virological Methods, 2016, 234:132-136.
[38] Jin W J, Yang Z, Zhao Z P, et al. Genetic characterization of porcine kobuvirus variants identified from healthy piglets in China[J]. Infection, Genetics Evolution, 2015, 35:89-95.
[39] 姬郭彪,赵军,周峰,等.河南地区猪嵴病毒分子检测和VP1基因遗传进化分析[J]. 中国预防兽医学报, 2014, 36(1):73-76.
[40] 易春华, 谢齐斌,唐薇薇,等. 猪嵴病毒VP1基因序列测定与分析[J]. 南方农业学报, 2016, 47(4):670-673.
[41] 陈如敬, 修金生, 陈晓珞,等. 猪嵴病毒VP0基因的克隆与序列分析[J]. 中国畜牧兽医, 2015, 42(9):2303-2307.
[42] Zhao Z P,Yang Z, Lin W D, et al. The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus[J]. Actavirol, 2016, 60(1):55-61.
[43] 李淞, 朱玲,段俊,等. 猪嵴病毒RT-PCR检测方法的建立及应用[J]. 中国兽医学报, 2013, 33(8):1150-1153.
[44] Reuter G,Boldizsa R A, Pankovics P. Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus kobuvirus, family picornaviridae[J]. Arch Virol, 2009, 154(1):101-108.
[45] 顾凡, 覃娟娟, 蔡雨函,等. 猪嵴病毒和猪星状病毒双重RT-PCR检测方法的建立[J]. 中国预防兽医学报, 2015, 37(4):279-281.
[46] Li C L, Chen J F, Shi H Y, et al. Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification[J]. Virology Journal, 2014, 11(1):73.
[47] 李长龙.猪嵴病毒全长cDNA感染性克隆的构建及拯救[D].北京:中国农业科学院, 2014.

Research Advances in Pathogenicity and Detection Techniques of Porcine Kobuvirus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	LIANG Yingxin, CHEN Zejia, LI Jichao, CUI Xiaoxiao, FENG Qianqi, LI Shoujun, CHEN Aolei. Investigation on the Infection of Equine Intestinal Parasite in Partial Cities in Guangdong Province [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2725-2734.
[3]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[4]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.
[5]	ZHANG Shuang, GUO Yu, XUE Wenhui, ZHAO Qianhui, HUO Shuying. Research Progress on Poultry Candida glabrata [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1013-1019.
[6]	SUN Jiali, DONG Hao, PENG Xiaowei, ZHU Liangquan, JIANG Hui, FENG Yu, LI Xiaoying, DING Jiabo, WANG Chunfeng. Research Progress on Brucella canis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3803-3811.
[7]	WANG Jiayun, WANG Xin, WANG Bingyu, ZENG Hao, SHEN Wenhui, YU Jiang, CHEN Zhi, ZHANG Yuyu, SUN Wenbo, GUO Lihui, REN Sufang, LI Yubao, WU Jiaqiang. Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1870-1881.
[8]	CHEN Qian, WANG Kunlun, YAN Yaqun, ZHOU Yongchun, ZHAO Cong, JIAN Fuchun, WANG Rongjun, ZHANG Longxian, NING Changshen. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Theileria luwenshuni in Sheep and Goats in Different Regions of China [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1902-1909.
[9]	LIU Hanyang, ZHANG Yi, XIAO Ran, CHEN Bin, WU Yongsheng, SU Zhipeng, LI Yupeng, PEI Chaoxin, XU Zhenying, ZHU Jiawen. Molecular Epidemiological Investigation on ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Sichuan in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1172-1182.
[10]	DUAN Qunpeng, ZHAO Shuo, QIN Yibin, LU Bingxia, HE Ying, CHEN Zhongwei, LIU Lei, ZHOU Yingning, LI Bin, JIANG Dongfu, LIANG Jiaxing, ZHAO Wu. Epidemiological Investigation Analysis of Porcine Circovirus Type 3 in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1183-1190.
[11]	YANG Chunjie, LIU Xueting, MI Xue, CHEN Ying, WEI Zuzhang, HUANG Weijian, OUYANG Kang. Epidemiological Investigation and VP1 Gene Sequence Analysis of Porcine Enterovirus Type G in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1216-1224.
[12]	DUAN Qunpeng, LI Xiaoyu, ZHAO Shuo, QIN Yibin, LU Bingxia, LI Bin, SU Qianlian, ZHAO Wu, HE Ying, LIANG Jiaxing, JIANG Dongfu, ZHOU Yingning, CHEN Zhongwei. A Epidemiological Investigation on Diarrhea Viral Disease of Piglets in Some Pig Farms in Guangxi During 2016 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 564-574.
[13]	LI Shaohan, ZHANG Guangzhi, CUI Shangjin, QIN Tong. Research Progress of Canine Coronavirus and Feline Coronavirus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4059-4068.
[14]	JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017 [J]. , 2019, 46(5): 1474-1482.
[15]	GUO Yingcheng, FU Shoupeng, GAO Xueyong, LI Xudong. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Porcine Circovirus Type 3 in Jilin Province [J]. , 2019, 46(5): 1483-1490.