金定鸭不同组织和血液中TLR1、TLR2、TLR4、TLR5的表达差异分析

doi:10.16431/j.cnki.1671-7236.2017.12.010

Abstract

Abstract:

The aim of this study was to explore the expression profiles of Toll-like receptor (TLR) in different tissues,and to lay the foundation of TLRs research for ducks.Ten male Jinding ducks of 300 days of age were slaughtered,and the blood sample and 14 different kinds of tissues (spleen, liver, testise, lung,hypothalamus,pituitary gland,skin,leg muscle,heart,kidney, chest muscle,caecum,small intestine and thymus) were collected. The Primer Premier 5.0 software was used to design specific primers and the quantitative Real-time PCR method was used to detect the relative expression levels of TLR1,TLR2,TLR4 and TLR5 mRNA. The results showed that there were only one specific peak on the melting curve of each gene amplification products,indicating that the specificity of the primers were strong. The amplification efficiency of the target genes was 101.4% to 105.0% and the correlation coefficient (R²) was 0.98 to 1.000. The four kinds of TLRs expressed in all of the 14 tissues and blood with varying abundance in different tissues for each of the 4 kinds of TLRs.The expression levels of TLR1 was lowest in hypothalamus,and highest in chest muscle. The expression levels of TLR2 was lowest in small intestine,and highest in lung. The lowest expressed tissues for TLR4 and TLR5 were testis, and the highest expressed tissues were skin.The study indicated that TLRs were widely expressed in various tissues of duck,which laid the foundation for further study on the role mechanism of duck TLRs in the process of pathogen infection.

Key words: Jinding duck; Toll-like receptor (TLR); tissues; relative expression

CLC Number:

Q786

XU Wen-juan, TAO Zhi-yun, ZHU Chun-hong, SONG Wei-tao, LIU Hong-xiang, ZHANG Shuang-jie, LI Hui-fang. Analysis of TLR1,TLR2,TLR4 and TLR5 Expression in Different Tissues of Jinding Duck[J]. , 2017, 44(12): 3459-3465.

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Analysis of TLR1,TLR2,TLR4 and TLR5 Expression in Different Tissues of Jinding Duck

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