PGD2/DP1途径对细菌感染奶牛子宫内膜组织中HMGB-1和PAFR表达的影响

doi:10.16431/j.cnki.1671-7236.2021.06.030

Abstract

Abstract: The aim of this study was to investigate the effects of PGD₂/DP1 receptor pathway on the expression of inflammatory mediators HMGB-1 and PAFR,and the degree of tissue injury in the endometrium of dairy cows infected with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).The endometrial tissues of cows were treated with 1×10^-6 mol/L DP₁ receptor agonists (BW-245C and 15d-PGJ2) and infected with E. coli and S. aureus at a concentration of 1×10⁶ CFU/mL.The expression of HMGB-1 and PAFR was detected by Real-time quantitative PCR,immunohistochemistry,and the tissue injury was evaluated by HE staining.The results showed that the expression of HMGB-1 and PAFR of endometrium tissue in dairy cows was significantly higher than that in blank control group after E. coli and S. aureus infection at a concentration of 1×10⁶ CFU/mL (P<0.05),while the expression of HMGB-1 and PAFR in bovine endometrium was significantly inhibited by DP₁ receptor agonists (P<0.05).HE staining showed that the endometrial epithelial cells and glandular epithelial cells was completely exfoliated,necrotic and disintegrated after E.coli and S.aureus infection.While the DP₁ receptor agonists treated bovine endometrium,it significantly reduced the degree of injury of bovine endometrium (P<0.05).The results of immunohistochemical staining were consistent with those of the above two methods.The results showed that PGD₂ could inhibit the expression of HMGB-1 and PAFR and reduce the degree of tissue damage in the bovine endometrium infected with E.coli and S. aureus,which might be mediated by DP₁ receptor.

Key words: bovine endometrial tissues; E.coli; S.aureus; inflammation; HMGB-1; PAFR

CLC Number:

S857.2⁺1

YANG Xiaolin, HAN Runlin, MAO Wei, BAO Haixia, LIU Kun, WU Jindi, CAO Jinshan, LIU Bo. Effect of PGD₂/DP₁ Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2169-2176.

References

[1] LI T T,LIU B,GUAN H,et al.PGE₂ increases inflammatory damage in Escherichia coli-infected bovine endometrial tissue in vitro via the EP4-PKA signaling pathway[J].Biology of Reproduction,2019,100(1):175-186.
[2] LEBLANC S J.Reproductive tract inflammatory disease in postpartum dairy cows[J].Animal,2014,8(s1):54-63.
[3] HAGMAN R,RONNBERG E,PEJLER G.Canine uterine bacterial infection induces upregulation of proteolysis-related genes and downregulation of homeobox and zinc finger factors[J].PLoS One,2009,4(11):e8039.
[4] SAWYER N,CAUCHON E,CHATEAUNEUF A,et al.Molecular pharmacology of the human prostaglandin D₂ receptor,CRTH₂[J].British Journal Pharmacology,2002,137(8):1163-1172.
[5] RICCIOTTI E,FITZGERALDG A.Prostaglandins and inflammation[J].Arteriosclerosis,Thrombosis and Vascular Biology,2011,31(5):986-1000.
[6] MURATA T,ARITAKE K,TSUBOSAKA Y,et al.Anti-inflammatory role of PGD₂ in acute lung inflammation and therapeutic application of its signal enhancement[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(13):5205-5210.
[7] 刘昆,毛伟,李婷婷,等.大肠杆菌与金黄色葡萄球菌对奶牛子宫内膜组织细胞因子表达及损伤程度的影响[J].中国畜牧兽医,2019,46(6):1841-1848. LIU K,MAO W,LI T T,et al.Effect to Escherichia coli and Staphylococcus aureus on expression of cytokine and damage of degree of dairy cows endometrium tissue[J].China Animal Husbandry ＆ Veterinary Medicine,2019,46(6):1841-1848.(in Chinese)
[8] 李婷婷,毛伟,刘博,等.大肠杆菌对奶牛子宫内膜组织合成分泌IL-1β、IL-6和PGE₂的影响[J].中国兽医学报,2019,39(6):1208-1213. LI T T,MAO W,LIU B,et al.Effects of Escherichia coli on synthesis and secretion of IL-1β,IL-6 and PGE₂ in dairy cow endometrium explants in vitro[J].Chinese Journal Veterinary Science,2019,39(6):1208-1213.(in Chinese)
[9] 张超,王灵芮,毛伟,等.EP₂受体介导大肠杆菌感染的奶牛子宫内膜组织损伤机制研究[J].黑龙江畜牧兽医,2019,23:11-14. ZHANG C,WANG L R,MAO W,et al.Study on the mechanism of endometrial tissue damage in dairy cows infected by Escherichia coli mediated by EP₂ receptor[J].Heilongjiang Animal Science and Veterinary Medicine,2019,23:11-14.(in Chinese)
[10] 王灵芮,刘昆,李婷婷,等.EP₄受体参与调控大肠杆菌感染后奶牛子宫内膜组织中IL-1β、IL-6、TNF-α的表达、分泌及组织损伤[J].黑龙江畜牧兽医,2019,22:68-72. WANG L R,LIU K,LI T T,et al.EP₄ receptor involved in the regulation of expression and secretion of IL-1β,IL-6 and TNF-α and tissue damage in bovine endometrium after E.coli infection[J].Heilongjiang Animal Science and Veterinary Medicine,2019,22:68-72.(in Chinese)
[11] 张超,李婷婷,毛伟,等.EP₂受体对大肠杆菌感染的奶牛子宫内膜组织中IL-1β和IL-6 mRNA表达的影响[J].中国兽医学报,2019,39(11):2200-2206. ZHANG C,LI T T,MAO W,et al.Effect of EP₂ receptor on IL-1β and IL-6 mRNA expression in Escherichia coli-infected bovine endometrial tissues[J].Chinese Journal Veterinary Science,2019,39(11):2200-2206.(in Chinese)
[12] DENG M,GUO H T,TAM J W,et al.Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR[J].Journal Experimental Medicine,2019,216(12):2838-2853.
[13] WANG E W,HAN Y Y,JIA X S.PAFR-deficiency alleviates myocardial ischemia/reperfusion injury in mice via suppressing inflammation,oxidative stress and apoptosis[J].Biochemical ＆ Biophysocal Research Communications,2018,495(4):2475-2481.
[14] ZHANG S Y,LIU B,MAO W,et al.The effect of prostaglandin E receptor (PTGER₂) activation on growth factor expression and cell proliferation in bovine endometrial explants[J].Prostaglandins Leukotrienes Essential Fatty Acids,2017,122:16-23.
[15] ZHANG S Y,LIU B,GAO L,et al.Prostaglandin F-PTGFR signaling activation,growth factor expression and cell proliferation in bovine endometrial explants[J].Reproduction Fertility Development,2017,29(11):2195-2205.
[16] LI T T,LIU K,MAO W,et al.Prostaglandin E promotes nitric oxide synthase 2,platelet-activating factor receptor,and matrix metalloproteinase-2 expression in Escherichia coli-challenged ex vivo endometrial explants via the prostaglandin E receptor 4/protein kinase a signaling pathway[J].Theriogenology,2019,134:65-73.
[17] 李宗波,付才,孔德江.奶牛子宫内膜炎研究进展[J].中国乳业,2010,2:62-65. LI Z B,FU C,KONG D J.Research progress of endometritis in cows[J].China Dairy,2010,2:62-65.(in Chinese)
[18] HATA A N,BREYER R M.Pharmacology and signaling of prostaglandin receptors:Multiple roles in inflammation and immune modulation[J].Pharmaco-logy Therapeutics,2004,103(2):147-166.
[19] SALILEW-WONDIM D,IBRAHIM S,GEBREMEDHN S,et al.Clinical and subclinical endometritis induced alterations in bovine endometrial transcriptome and miRNome profile[J].BMC Genomics,2016,17:218.
[20] GERBAIS F G,CRUZ R P,CHATEAUNEUF A,et al.Selective modulation of chemokinesis,degranulation,and apoptosis in eosinophils through the PGD₂ receptors CRTH₂ and DP[J].Journal of Allergy and Clinical Immunology,2001,108(6):982-988.
[21] MONNERET G,GRAVEL S,DIAMIND M,et al.Prostaglandin D₂ is a potent chemoattractant for human eosinophils that acts via a novel DP receptor[J].Blood,2001,98(6):1942-1948.
[22] HATAI H,TANAKA K,YOSHIE O,et al.Prostaglandin D₂ selectively induces chemotaxis in T helper type 2 cells,eosinophils,and basophils via seven-transmembrane receptor CRTH₂[J].Journal of Experimental Medicine,2001,193(2):255-261.
[23] ANGELI V,STAUMONT D,CHARBONNIER A S,et al.Activation of the D prostanoid receptor 1 regulates immune and skin allergic responses[J].Journal of Immunology,2004,172(2):3822-3829.
[24] YOSHIMURA-UCHIYAMA C,IIKURA M,YAMAGUCHI M,et al.Differential modulation of human basophil functions through prostagland in D₂ receptors DP and chemoattractant receptor-homologous molecule expressed on Th2 cells/DP₂[J].Clinical Experimental Allergy,2004,34:1283-1290.
[25] VIJAY R,FEHR A R,JANOWSKI A M,et al.Virus-induced inflammasome activation is suppressed by prostaglandin D₂/DP₁ signaling[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(27):E5444-E5453.
[26] KOGA M M,BIZZARRO B,SA-NUNES A,et al.Activation of PAF-receptor induces regulatory dendritic cells through PGE₂ and IL-10[J].Prostaglandins Leukotrienes Essential Fatty Acids,2013,89(5):319-326.
[27] SUGANO T,NARAHARA H,NASU K,et al.Effects of platelet-activating factor on cytokine production by human uterine cervical fibroblasts[J].Molecular Human Reprod,2001,7(5):475-481.
[28] KIM H,JUNG B J,JEONG J,et al.Lipoteichoic acid from Lactobacillus plantarum inhibits the expression of platelet-activating factor receptor induced by Staphylococcus aureus lipoteichoic acid or Escherichia coli lipopolysaccharide in human monocyte-like cells[J].Journal of Microbiology and Biotechnology,2014,24(8):1051-1058.

Effect of PGD₂/DP₁ Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria

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Effect of PGD2/DP1 Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria

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Effect of PGD₂/DP₁ Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria